Prebiotic activity of Lactobacillus casei grown on medium containing of Hylocereus undatus extract and its use in the fermentation of goat’s milk kefir




Abstract. Kustyawati ME, Nurlita ME, Fadhallah EG, Rizal S. 2022. Prebiotic activity of Lactobacillus casei grown on medium containing of Hylocereus undatus extract and its use in the fermentation of goat’s milk kefir. Biodiversitas 23: 6513-6519. White dragon fruit extract used as a prebiotic source is thought to be an innovative exploration to enhance its application as a synbiotics in food products. This study aims to quantify the prebiotic index and prebiotic activity score for Lactobacillus casei grown on white dragon fruit extract containing media at different concentrations. The study was carried out using a completely randomized design (CRD) experimental method with one factor, concentration of white dragon fruit extract (P1-P5) with 5 levels (2, 4, 6, 8, and 10% respectively), and was repeated 3 times. The data were analyzed statistically by ANOVA, if the treatments were significantly different then the LSD test (5%). The result showed that all tested media had a beneficial effect on the growth of L. casei (prebiotic index higher than 1), while they did not support the growth of enteric Escherichia coli (prebiotic index less than 1 or negative). The highest score of prebiotic activity for L. casei was grown on 10%, while the lowest was 2%. The addition of 10% prebiotic extract of white dragon fruit in goat's milk fermentation produced goat's milk kefir with characteristics that met Codex Stand 243-2003 and had a DPPH radical scavenging activity of 55.13%.


Bandiera, N.S. et al. (2013) ‘Viability of probiotic Lactobacillus casei in yoghurt: Defining the best processing step to its addition’, Archivos Latinoamericanos de Nutricion, 63(1), pp. 58–63.
BUDHISATRIA, R. et al. (2017) ‘In Vitro and In Vivo Prebiotic Activities of Purified Oligosaccharides Derived from Various Local Bananas (Musa sp.): Tanduk, Uli, Raja Sereh, and Cavendish’, Microbiology Indonesia, 11(2), pp. 55–61. doi:10.5454/mi.11.2.3.
Dahiya, D. and Nigam, P.S. (2022) ‘Probiotics, Prebiotics, Synbiotics, and Fermented Foods as Potential Biotics in Nutrition Improving Health via Microbiome-Gut-Brain Axis’, Fermentation, 8(7). doi:10.3390/fermentation8070303.
Figueroa-González, I. et al. (2019) ‘Prebiotic effect of commercial saccharides on probiotic bacteria isolated from commercial products’, Food Science and Technology (Brazil), 39(3), pp. 747–753. doi:10.1590/fst.07318.
Gibson, G.R. et al. (2004) ‘Dietary modulation of the human colonic microbiota: updating the concept of prebiotics’, Nutrition Research Reviews, 17(2), pp. 259–275. doi:10.1079/nrr200479.
Huebner, J., Wehling, R.L. and Hutkins, R.W. (2007) ‘Functional activity of commercial prebiotics’, International Dairy Journal, 17(7), pp. 770–775. doi:10.1016/j.idairyj.2006.10.006.
Jerônimo, M.C. and Costa Orsine, J.V. (2015) ‘Chemical and Physical-Chemical Properties, Antioxidant Activity and Fatty Acids Profile of Red Pitaya [Hylocereus Undatus (Haw.) Britton & Rose] Grown In Brazil’, Journal of Drug Metabolism & Toxicology, 06(04), pp. 6–11. doi:10.4172/2157-7609.1000188.
Kustyawati, M.E. et al. (2021) ‘Quality and Shelf Life of White Shrimp (Litopenaeus vannamei) Processed with High-Pressure Carbon Dioxide (HPCD) at Subcritical and Supercritical States’, Journal of Food Quality, 2021. doi:10.1155/2021/6649583.
Luu, T.T.H. et al. (2021) ‘Dragon fruit: A review of health benefits and nutrients and its sustainable development under climate changes in Vietnam’, Czech Journal of Food Sciences, 39(2), pp. 71–94. doi:10.17221/139/2020-CJFS.
de Melo Pereira, G.V. et al. (2014) ‘Isolation, selection and evaluation of yeasts for use in fermentation of coffee beans by the wet process’, International Journal of Food Microbiology, 188, pp. 60–66. doi:10.1016/j.ijfoodmicro.2014.07.008.
Palframan, R., Gibson, G.R. and Rastall, R.A. (2003) ‘Development of a quantitative tool for the comparison of the prebiotic effect of dietary oligosaccharides’, Letters in Applied Microbiology, 37(4), pp. 281–284. doi:10.1046/j.1472-765X.2003.01398.x.
Pansai, N. et al. (2020) ‘Gut microbiota modulation and immune boosting properties of prebiotic dragon fruit oligosaccharides’, International Journal of Food Science and Technology, 55(1), pp. 55–64. doi:10.1111/ijfs.14230.
Parvez, S. et al. (2006) ‘Probiotics and their fermented food products are beneficial for health’, Journal of Applied Microbiology, 100(6), pp. 1171–1185. doi:10.1111/j.1365-2672.2006.02963.x.
Patel, S. and Goyal, A. (2012) ‘Recent developments in mushrooms as anti-cancer therapeutics: a review’, 3 Biotech, 2(1), pp. 1–15. doi:10.1007/s13205-011-0036-2.
Rubel, I.A. et al. (2014) ‘In vitro prebiotic activity of inulin-rich carbohydrates extracted from Jerusalem artichoke (Helianthus tuberosus L.) tubers at different storage times by Lactobacillus paracasei’, Food Research International, 62, pp. 59–65. doi:10.1016/j.foodres.2014.02.024.
Song, H. et al. (2016) ‘White pitaya (Hylocereus undatus) juice attenuates insulin resistance and hepatic steatosis in diet-induced obese mice’, PLoS ONE, 11(2), pp. 1–14. doi:10.1371/journal.pone.0149670.
Vasiljevic, T. and Shah, N.P. (2008) ‘Probiotics-From Metchnikoff to bioactives’, International Dairy Journal, 18(7), pp. 714–728. doi:10.1016/j.idairyj.2008.03.004.

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