Incidence of Escherichia coli producing Extended-spectrum beta-lactamase in wastewater of dairy farms in East Java, Indonesia
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Abstract
Abstract. Dameanti FNAP, Yanestria SM, Widodo A, Effendi MH, Plumeriastuti H, Tyasningsih W, Sutrisno R, Akramsyah MA. 2023. Incidence of Escherichia coli producing Extended-spectrum beta-lactamase (ESBL) in wastewater of dairy farms in East Java, Indonesia. Biodiversitas 24: 1143-1150. Poor wastewater treatment on dairy farms can potentially become environmental contaminants (hazardous chemicals, organic matter, and pathogenic bacteria). Bacteria from dairy cattle that have been resistant can be found in wastewater. It can migrate around the farm and cause resistance to environmental microbiota. Over the years, cases of antimicrobial resistance (AMR) in Escherichia coli bacteria are increasing due to the uncontrolled use of antibiotics in dairy farms. The present study aimed to identify the potential occurrence of dairy farm wastewater as a reservoir for Extended-Spectrum Beta-Lactamase (ESBL)-producing E. coli in East Java. The number of research samples used was 342 and came from 6 cities/regencies with the highest dairy cattle population in East Java (Pasuruan District, Malang District, Tulungagung District, Blitar District, Batu City, and Kediri District). The results showed that 69.30% (237/342) of dairy farm wastewater samples were positive for E. coli. The incidence of Escherichia coli AMR was 99.17% (235/237). The resistance conditions to each class of antibiotics were 76.4% ampicillin, 66.2% cefotaxime, 37.6% tetracycline, 15.6% ciprofloxacin, 96.2% streptomycin, 16.5% sulfamethoxazole-trimethoprim, and 84.0% chloramphenicol. The incidence of E. coli MDR was 84.25% (198/235), with the highest incidence (44.44%; 88/198) found in the four groups of antibiotics. The incidence of ESBL-producing E. coli from samples of dairy farm wastewater in East Java through DDST confirmation was 22.80% (78/342), with the highest incidence (20.51%; 16/78) in Pasuruan District and Batu City. In conclusion, dairy farm wastewater could be a reservoir for ESBL-producing E. coli which has the potential to impact human health in East Java Province.
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References
Agnes S, Ricke L, and Muzahar. 2018. Beberapa Faktor Resiko pada Pasien dengan Infeksi oleh E. coli dan K. pneumoniae Penghasil ESBL di RSUP H. Adam Malik Medan. Majalah Kedokteran Nusantara 51(4): 185-190.
Amine AEK. 2013. Extended Spectrum Beta-Lactamase Producing Bacteria in Waste Water Alexandria, Egypt. International Journal of Bioscience, Biochemistry and Bioinformatics 3(6): 605 – 609.
Antimicrobial Resistance Collaborators. 2022. Global burden of bacterial antimicrobial resistance in 2019: a systematic analysis. The Lancet 399(55): 629-655.
Astorga F, Talloni MJN, Miró MP, Bravo M, Toro M, Blondel CJ, and Claude LPH. 2019. Antimicrobial Resistance in E. Coli Isolated from Dairy Calves and Bedding Material. Heliyon 5(11): 1 – 6.
Biutifasari W. 2018. Extended Spectrum Beta-Lactamase (ESBL). Oceana Biomedicina Journal 1(1): 1-11.
Blaak H, Lynch G, Italiaander R, Hamidjaja RA, Schets FM, and de Roda Husman AM. 2015. Multidrug-Resistant and Extended Spectrum Beta-Lactamase Producing Escherichia coli in Dutch Surface Water and Wastewater. PLoS One 10(6).
Canton R, Gonzalez-Alba JM, and Galan JC. CTX-M Enzymes: Origin and Diffusion. Front Microbiol Review 3(110): 1-19.
Cappuccino JG, and Welsh C. 2020. Microbiology A Laboratory 12th Edition Global Edition. Pearson. England.
Chu W, Zere TR, Weber MM, Wood TK, Whiteley M, and McLean RJC. 2012. Indole production promotes Escherichia coli mixed-culture growth with Pseudomonas aeruginosa by inhibiting quorum signaling. Applied and Environmental Microbiology 78(2): 411–419.
Chukwunwejim CR, Eze PM, Ujam NT, Abonyi IC, and Ejikeugwu CP. 2018. Incidence of Community-Acquired ESBL-producing Bacteria among Asymptomatic University Students in Anambra State, Nigeria. European Journal of Biological Research 8(3): 138 – 147.
Clinical and Laboratory Standards Institute. 2020. Performance standards for antimicrobial susceptibility testing in CLSI supplement M100. In. Wayne, PA: CLSI.
Deshpande PR, Rajan S, Sudeepthi BL, and Nasir CPA. 2011. Patient-Reported Outcomes: A New Era in Clinical Research. Perspect Clin Res 2(4): 137-144.
Dinas Peternakan Provinsi Jawa Timur. 2020. Statistika Populasi Ternak 2020. Disnak jatimprov.
Food and Agriculture Organization (FAO). 2018. Antimicrobial Resistance in the Environment. http:/www.fao.org.
Franz E, Veenman C, van HA, Husman AR, and Blaak H. 2015. Pathogenic Escherichia coli Producing Extended Spectrum ?-Lactamases Isoated from Surface Water and Wastewater. Scientific Reports 5 : 19.
He Y, Yuan Q, Mathieu J, Stadler L, Senehi N, Sun R, and Alvarez PJJ. 2020. Antibiotic resistance genes from livestock waste: occurrence, dissemination, and treatment. npj Clean Water 3(4): 1-4.
Hidayatullah, Gunawan, Mudikdjo K, and Erliza N. 2005. Pengelolaan Limbah Cair Usaha Peternakan Sapi Perah Melalui Penerapan Konsep Produksi Bersih. Jurnal Pengkajian dan Pengembangan Teknologi Pertanian 8(1): 124-136.
Imasari T, Tyasningsih W, Wasito EB, and Kuntaman K. 2018. Prevalensi dan Pola Gen Extended Spectrum ?-lactamase Bakteri Usus Sapi Perah dan Penduduk Sekitar Peternakan di Surabaya. Jurnal Veteriner 19(3): 313 - 320.
Indriani AP, Muktiani A, and Pangestu E. 2013. Konsumsi dan Produksi Protein Susu Sapi Perah Laktasi Yang Diberi Suplemen Temulawak (Curcuma xanthorrhiza) dan Seng Proteinat. Animal Agriculture Journal 2 (1): 128 – 135.
International Monetary Fund. 2014. Global Health Threats of the 21st Century. International Monetary Fund (IMF) 51(4). https://www.imf.org.
Leboffe MJ, and Pierce CBE. 2011. A Photographic Atlas for the Microbiology Laboratory. 4th EDITION. USA: Morton Publishing Company.
Lal A, and Cheeptham N. 2017. Eosin Methylene Blue Agar Protocol. ML Library. American Society for Microbiology.
Masruroh CA, Sudarwanto MB, and Latif H. 2016. Tingkat Kejadian Escherichia coli Penghasil Extended Spectrum–Lactamase yang Diisolasi dari Feses Broiler di Kota Bogor. Jurnal Sain Veteriner 34(1): 42-49.
Manuaba IASP, Iswari IS, Pinatih KJP. 2021. Prevalensi Bakteri Escherichia coli dan Klebsiella pneumoniae Penghasil Extended Spectrum Beta Lactamase (ESBL) Yang Diisolasi Dari Pasien Pneumonia Di RSUP Sanglah Periode Tahun 2019-2020. Jurnal Medika Udayana 10(12): 51- 57.
Maulana KT, Pichpol D, Farhani NR, Widiasih DA, Unger F, Punyapornwithaya V, dan Meeyam T. 2021. Antimicrobial resistance characteristics of Extended Spectrum Beta Lactamase (ESBL) producing Escherichia coli from dairy farms in the Sleman district of Yogyakarta province, Indonesia. Veterinary Integrative Sciences 19(3): 525-535.
Munawir. 2017. Deteksi Karier Gen Penyandi Extended Spectrum Betalactamase Dari Spesimen Feses Dan Isolat Escherichia Coli yang Diisolasi Dari Spesimen yang Sama Pada Siswa Sekolah Dasar Di Sidenreng Rappang, Sulawesi Selatan. [Thesis]. Universitas Hasanuddin. Makassar. [Indonesian].
Niasono AB, Latif H, and Purnawarman, T. 2019. Resistensi Antibiotik terhadap Bakteri Escherichia coli yang Diisolasi dari Peternakan Ayam Pedaging di Kabupaten Subang, Jawa Barat. Jurnal Veteriner 20 (2): 187-195.
Normaliska R, Sudarwanto BM, and Latif H. 2019. Pola Resistensi Antibiotik pada Escherichia coli Penghasil ESBL dari Sampel Lingkungan di RPH-R Kota Bogor. Jurnal Acta Veterinaria Indonesia 7(2): 42 – 48.
Putra RS, Effendi MH, Koesdarto S, Suwarno, Tyasningsih W, and Estoepangestie ATS. 2019. Identifikasi Bakteri Escherichia coli Penghasil Extended Spectrum ?-Lactamase Dari Swab Rectal Sapi Perah Menggunakan Metode Vitek-2 Di Kud Tani Wilis Sendang Kabupaten Tulungagung. Journal of Basic Medicine Veterinary 8(2): 108-114.
Saekhow P, and Sriphannam X. 2021. Prevalence of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli Strains in Dairy Farm Wastewater in Chiang Mai. Veterinary Integrative Sciences 19(3): 349–362.
Said LB, Jouini A, Klibi N, Dziri N, Alonso CA, Boudabous A, Ben SK, and Torres C. 2015. Detection of Extended-Spectrum beta-lactamase (ESBL) Producing Enterobacteriaceae in Vegetables, Soil and Water of the Farm Environment in Tunisia. International Journal of Food Microbiology 203: 86 – 92.
Soekoyo AR, Sulistiawati, Setyorini W, and Kuntaman K. 2020. The Epidemiological Pattern and Risk Factor of ESBL (Extended Spectrum ?-Lactamase) Producing Enterobacteriaceae in Gut Bacterial Flora of Dairy Cows and People Surrounding in Rural Area, Indonesia. Indonesian Journal of Tropical and Infectious Disease 8(3): 144–151.
Soré S, Sawadogo Y, Bonkoungou JI, Kaboré SP, and Béogo S. 2020. Detection, identification, and characterization of extended-spectrum beta-lactamases producing Enterobacteriaceae in wastewater and salads marketed in Ouagadougou, Burkina Faso. Int. J. Biol. Chem. Sci 14(8): 2746-2757.
Ulfah NF, Erina, and Darniati. 2017. Isolation and Identification Escherichia coli in Roasted Chicken from Restaurantin Syiah Kuala, Banda Aceh. Jurnal Ilmu Medik Veteriner 01(3): 383-390.
Wibisono FJ, Sumiarto B, Untari T, Effendi MH, Permatasari DA, and Witaningrum M. 2020. CTX Gene of Extended Spectrum Beta-Lactamase (ESBL) Producing Escherichia coli on Broilers in Blitar, Indonesia. Sys Rev Pharm 11(7): 396-403.
Wibisono FJ, Sumiarto B, Untari T, Effendi MH, Permatasari DA, and Witaningrum M. 2020.Short Communication: Extended spectrum beta-lactamase (ESBL) producing Escherichia coli on layer chicken farms in Blitar Area, Indonesia. Biodiversitas Journal of Biological Diversity 21(6): 2667-2671.
Widhi APKN, and Saputra IY. 2021. Residu Antibiotik Serta Keberadaan Escherichia Coli Penghasil ESBL pada Daging Ayam Broiler di Pasar Kota Purwokerto. Jurnal Kesehatan Lingkungan Indonesia 20(2): 137-142.
Woolhouse M, Ward M, van Bunnik B, and Farrar J. 2015. Antimicrobial Resistance in Humans, Livestock and The Wider Environment. Phil Trans R Soc B 370: 1-7.
World Health Organization. 2012. The Evolving Threat of Antimicrobial Resistance: Options for Action. Geneva: World Health Organisation.
World Health Organization. 2019. New report calls for urgent action to avert antimicrobial resistance crisis. Joint News Release. https://www.who.int.
World Health Organization. 2021. WHO integrated Global Surveillance on ESBL-Producing E. coli using a “One Health” Approach: Implementation and Opportunities. https://www.who.int.
Yanestria SM, Dameanti FNAEP, Musayannah BG, Pratama JWA, Witaningrum AM, Effendi4 MH, and ugbo EN. 2022. Antibiotic resistance pattern of Extended-Spectrum ?-Lactamase (ESBL) producing Escherichia coli isolated from broiler farm environment in Pasuruan district, Indonesia. BIODIVERSITAS 23(9): 4460-4465.
Yanestria SM, Rahmaniar RP, Wibisono FJ, and Effendi MH. 2019. Detection of invA gene of Salmonella from Milkfish (Chanos chanos) at Sidoarjo Wet Fish Market, Indonesia, Using Polymerase Chain Reaction Technique. Vet.World 12(1): 170-175.
Zhang H, Gao Y, and Chang W. 2016. Comparison of Extended-Spectrum ????- Lactamase Producing Escherichia coli Isolates from Drinking Well Water and Pit Latrine Wastewater in a Rural Area of China. BioMed Research International: 1-7.
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