Diversity of actinomycetes on plant rhizosphere of karst ecosystem of Gorontalo, Indonesia




Abstract. Retnowati Y, Kandowangko NY, Katili AS, Pembengo W. 2024. Diversity of actinomycetes on plant rhizosphere of karst ecosystem of Gorontalo, Indonesia. Biodiversitas 25: 907-915. The objective of this study was to isolate and identify the actinomycetes associated with the rhizosphere of plants in the karst ecosystem. This study was descriptive-quantitative. Soil sampling was conducted at three locations of the karst ecosystem of Gorontalo, i.e., Bangga Hills at Bangga Bubaa Coastal area of Gorontalo District, Hills around Lake Limboto of Gorontalo District, and Oluhuta Beach Hills at Bone Bolango District. Rhizosphere-soil sample was collected from 15-30 cm of soil depth. Isolation of actinomycetes was performed using the plate method. The screening of phosphate-solubilizing bacteria was based on the ability to solubilize phosphates indicated by the clear zone on the Pikovskaya medium. Identification of actinomycetes bacteria based on morphology and molecular characters. The morphology character included the color of aerial and substrate mycelium. Molecular characteristics based on the sequence of 16S rRNA gene compared to sequence data on the gene bank of NCBI. The phylogenetic tree reconstruction was based on the Neighbor-joining algorithm. The results successfully found six isolates, including three actinomycetes isolates, two non-actinomycetes bacteria, and one yeast. They were isolated from the rhizosphere of Malastoma malabathrum, Chromolaena odorata, Cycas rumphii, Leucaena leucocephala, and Jatropha curcas. The actinomycetes isolate showed plant-growth-promoting potential indicated by solubilizing phosphate and Indole 3-Acetic Acid production activities. Based on the molecular analysis, actinomycetes were closely related to Streptomyces aegyptia (MT505707.1:42-1426), Streptomyces sp. GGCR-6 (MH718844.1:5-1391), and Streptomyces carpaticus strain PES-A23 (MH712039).


Ahemada M., and Kibret M . 2014. Mechanisms and applications of plant growth promotingrhizobacteria: Current perspective. Journal of King Saud University –Science.26. 1-20
Anwar S., Ali B., and Sajid. I. 2016. Screening or rhzopheric actinomycetes for various in-vitro and in-vivo plant growth promoting (PGP) traits and agroactive compounds. Frontiers in microbiology. Vol 7. Pp. 1334-1345.
Bhattacharyya P.N., and Jha D.K. 2012. Plant growth-promoting rhizobacteria (PGPR): emergence in agriculture. World Journal of Microbiology and Biotechnology. Volume 28, Issue 4, pp 1327–1350
Chitraselvi P.E.R. 2018. Actinomycetes: Dependable Tool for Sustainable Agriculture. Current investigation in agriculture and current research. Volume 1 - Issue 5
Clarholm M., Skyllberg U., Rosling A. (2015). Organic acid induced release of nutrients from metal-stabilized soil organic matter - The unbutton model. Soil Biol. Biochem. 84 168–176. 10.1016/j.soilbio.2015.02.019 [CrossRef] [Google Scholar]
Franco-Correa M and Chavarro-Anzola V. 2015. Actinobacteri as plant growth-promoting rhizobacteria. Basic and Biotechnology aplications.
Ganapathy A., and Natesan S. 2018. Metabolic Potential and Biotechnological Importance of Plant Associated Endophytic Actinobacteria. New and Future Developments in Microbial Biotechnology and Bioengineering Actinobacteria: Diversity and Biotechnological Applications, 2018, Pages 207-224
Goudjal Y., Zamoum M., Sabaou N., and Zitouni A. 2018. Endophytic Actinobacteria from Native Plants of Algerian Sahara: Potential Agents for Biocontrol and Promotion of Plant Growth. New and Future Developments in Microbial Biotechnology and Bioengineering Actinobacteria: Diversity and Biotechnological Applications, Pages 109-124
Glick B.R. 2012. Plant Growth-Promoting Bacteria: Mechanisms and Applications. Scientifica. Volume 2012, Article ID 963401. http://dx.doi.org/10.6064/2012/963401
Hamdali H., Joelle-Virolle M., Hafidi M., Nand Ouhdouch Y. 2008. Roch phosphate solubilizing actinomycetes: Screening for plant growth promoting activities. World J. Microbiol. Biotechnol. 24: 2565-2575.
Hu L. N., Su Y. R., He X. Y., Wu J. S., Zheng H., Li Y., et al. (2012). Response of soil organic carbon mineralization in typical Karst soils following the addition of 14C-labeled rice straw and CaCO3. J. Sci. Food Agr. 92 1112–1118. 10.1002/jsfa.4647 [PubMed] [CrossRef] [Google Scholar]
Katili A.S., and Retnowati Y. 2017. Isolation of actinomycetes from mangrove ecosystem in Torosiaje, Gorontalo, Indonesia. Biodiversitas., 18(2):826-833.
Majeed A., Muhammad Z., Ahmad H. 2018. Plant growth promoting bacteria: role in soil improvement, abiotic and biotic stress management of crops. Plant Cell Rep. 37(12):1599-1609.
Merzaeva O.V and I.G. Shirokikh. 2006. Colonization of plant rhizosphere by actinomycetes of different genera. Microbiology volume 75, pages226–230
Niyomvong N., Pathom-aree W., Thamchaipenet A., and Duangmal K. 2012. Actinomycetes from Tropical Limestone Caves. Chiang Mai J. Sci. 2012; 39(3) : 373-388 http://it.science.cmu.ac.th/ejournal/ Contributed Paper Pan F., Liang Y., Zhang W., Zhao J.,and Wang K3,* 2018. Enhanced Nitrogen Availability in Karst Ecosystems by Oxalic Acid Release in the RhizosphereFront Plant Sci. 2016; 7: 687.
Rangseekaew P and Pathom-aree W. 2019;.Cave Actinobacteria as Producers of Bioactive Metabolites Front Microbiol. 10: 387
Retnowati Y., Moeljopawiro S., Djohan T.S., and Soetarto E.S. 2018. Antimicrobial activities of actinomycete isolates from rhizospheric soils in different mangrove forests of torosiaje, gorontalo, indonesia. Biodiversitas. 9(6): 2196-2203
Sathya A., Vijayabharathi R, and Gopalakrishnan S. 2017. Plant growth-promoting actinobacteria: a new strategy for enhancing sustainable production and protection of grain legumes. 3 Biotech. 2017 Jun; 7(2): 102.
Singh D,P., Patil H,J., Prabha R., Yandigeri M.S., and Prasad S.R. 2018. Actinomycetes as Potential Plant Growth-Promoting Microbial Communities. Crop improvement through microbial biotechnology. Pp. 27-28
Spaepen, S., and Vanderleyden, J., 2011. Auxin and plant-microbeinteractions. Cold Spring Harb. Perspect. Biol. ;3:a001438
Sreevidya M., Gopalakhrisnan S., Kudapa H., and Varshney R.K., 2015. Exploring plant growth-promotion actinomycetes from vermicompost and rhizosphere soil for yield enhancement in chickpea. Brazilian Journal of Microbiology. 47. Pp. 85-95.
Wahyudi A.T., Priyanto J.A., Afrista R., Kurniati D., Astuti R.I., and Akhdiya A. 2019. Plant Growth Promoting Activity of Actinomycetes Isolated from Soybean Rhizosphere. OnLine Journal of Biological Sciences. volume 19, Issue 1.Pages 1-8
Wang X, J., Zhang J; Wang J.D., Qian P,T., Liu C.X., and Xiang W.S. 2013. Novel cyclopentnone derivates produced by a rare actinobacterial strain Actinoalloteichus nanshanensi sp. nov. NEAU 119. Nat. Prod. Res. 27. 1863-3869
Yurnaliza Y., Priyani N dan Wildasari N. 2011. Peran Bakteri Endofit Penghasil IAA (Indol Acetic Acid) Terseleksi Terhadap Pertumbuhan Tanaman Padi (Oryza sativa L.). Prosiding seminar Biologi . https://www.researchgate.net/publication/323390414
Damam M., Moinudin M.K., and Kausar R. 2016. Isolation and screening plant growth promoting actinomycetes from some forest medicininal plants. International journal of chemtech research. 9(5):521-528
Karthikeyan N., Pandiyan K., Suhu P.K., Srinivasan N., and Singh U.B. 2018. Actinomycetes: a promising tool for plant growth promotion and disease control. International journal of current microbiology and applied science. 7(7):2418-2429.
Erraki R., Lebrihi H., and Kahkahi R.E. 2014. Isolation and screening or actinomycetes strains producing plant growth promoting.
Kamal R., Gusein Y.S., and Kumar V. 2014. Interaction and symbiosis AM fungi, Actinomycetes and plant growth promotion rhizobacteria with plant: a strategies for the improvement of plant health and defense system. International journal of microbiology and applied science. 3(7):564-585.
Sreevidya M, Gopalakrishnan S, Kudapa H, Varshney RK. 2016. Exploring plant growth-promotion actinomycetes from vermicompost and rhizosphere soil for yield enhancement in chickpea. Brazilian Journal of Microbiology. Vol. 47 (1):85-95
Novianti D. 2018. Perbanyakan Jamur Trichoderma sp pada Beberapa Media. Sainmatika: Jurnal Ilmiah Matematika dan Ilmu Pengetahuan Alam. Vol.15 (1): 35-41.

Most read articles by the same author(s)

1 2 > >>