Effect of Lactobacillus casei FNCC 0090 to improve gastrointestinal bacterial abundance, immune system and water quality in catfish farming
##plugins.themes.bootstrap3.article.sidebar##
Issue
Section
Articles
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
##plugins.themes.bootstrap3.article.main##
NURUL ‘AINI
https://orcid.org/0009-0003-0685-9908
MUHAMMAD BACHRUDDIN
FATIMAH
https://orcid.org/0000-0002-9831-2154
SAPTO ANDRIYONO
https://orcid.org/0000-0002-2566-1636
SRI PUJI ASTUTI WAHYUNINGSIH
♥
Abstract
Abstract. ‘Aini N, Bachruddin M, Fatimah, Andriyono S, Wahyuningsih SPA. 2024. Effect of Lactobacillus casei FNCC 0090 to improve gastrointestinal bacterial abundance, immune system and water quality in catfish farming. Biodiversitas 25: 2130-2138. Catfish is the most widely cultivated type of fish. However, along with the increase in catfish production as an effort to fulfill the fishery stock, there is an obstacle to the emergence of disease infection caused by Aeromonas hydrophila. For this reason, good fisheries management is required. One of the efforts to deal with this is by giving probiotics Lactobacillus casei FNCC 00990. Lactobacillus casei FNCC 0090 is expected to improve the immune system of catfish through improving the blood profile of fish and increasing the number of lactic acid bacteria in the gastrointestinal tract of catfish. This research aims to determine the effect of administering the probiotic L. casei FNCC 0090 on the abundance of microbes in the fish digestive tract, immune system, and water quality in cultivating catfish infected with A. hydrophila. The research design was a completely randomized design with five treatments and five replications. The treatments were A (administering 5% probiotics and infected with 0.1 mL x 108 CFU/mL), B (10% probiotics and infected), C (15% probiotics and infected), KP (positive control with 0% probiotics and infected with pathogens), KN (negative control, without probiotics and pathogen infection). Fish are kept for 42 days; on the 35th day, the fish were infected with A. hydrophila by intramuscular injection. Probiotics were mixed with feed with a total of 108 CFU/mL. Data in the form of BAL counts and total bacterial counts of fish digestive tracts, blood profiles, and water quality were analyzed using Statistical Package for the Social Sciences (SPSS) software version 23.0 (IBM SPSS Statistics). The results showed
##plugins.themes.bootstrap3.article.details##
References
Aini N, Bachruddin M, Tsana I. 2020. Microbial isolation from gastrointestinal tract of catfish (Clarias gariepinus). Journal of Microbial Systematics and Biotechnology 2(1): 22-30. DOI:10.37604/jmsb.v2i1.38
Aini N, Hariani D. 2019. Effect of pineapple peel filtrate and probiotic Lactobacillus casei FNCC 0090 on catfish growth (Clarias gariepinus). AIP Conference Proceedings, The 9th International Conference On Global Resource Conservation (ICGRC) and Aji from Ritsumeikan University DOI: 10.1063/1.5061910
Apriliyanto T, Anindita NS, Susila WA. 2021. Antibacterial activity of isolates from digestive tract of catfish (Clarias gariepinus) as probiotic candidates at PT. Biotech Cipta Kreasi. International Journal of Health Science and Technology 3(1): 138–149. DOI: 10.31101/ijhst.v3i1.2240
Aritonang SN, Roza E, Rossi E, Purwati E, dan Husmaini H, 2017. Isolation and identification of lactic acid bacteria from okara and evaluation of their potential as candidate probiotics. Pakistan Journal of Nutrition 16 8: 618-628. DOI: 10.3923/pjn.2017.618.628
Burgos FA, Ray CL, Arias CR. 2018. Bacterial diversity and community structure of the intestinal microbiome of channel catfish (Ictalurus punctatus) during ontogenesis. Systematic and Applied Microbiology 41: 494-505. DOI: 10.1016/j.syapm.2018.04.006
Cavalcante RB, Telli GS, Tachibana L, Dias DC, Oshiro E, Natori MM, da Silva WF, Jose M, Ranzani PMJ. 2020. Probiotics, prebiotics and synbiotics for nile tilapia: growth performance and protection against Aeromonas hydrophila infection. Aquaculture Reports 17 100343. DOI: 10.1016/j.aqrep.2020.100343
Cho JH, Jin WP, Yong WR, Kang WK, Sang WH. 2023. Morphology, histology, and histochemistry of the digestive tract of the marbled flounder Pseudopleuronectes yokohamae. Animals 13 (5): 936. DOI: 10.3390/ani13050936
Choi W, Moniruzzaman M, Bae J, Hamidoghli A, Lee S, Choi YH, Min T, Bai SC. 2022. Evaluation of dietary probiotic bacteria and processed yeast (GroPro-Aqua) as the alternative of antibiotics in juvenile olive flounder Paralichthys olivaceus. Antibiotics (Basel) 11(2):129. DOI: 10.3390/antibiotics11020129
Dawood MAO. 2021. Nutritional immunity of fish intestines: important insights for sustainable aquaculture. Reviews in Aquaculture 13: 642–663. DOI: 10.1111/raq.12492
Diwan AD, Harke SN, Panche AN. 2023. Host-microbiome interaction in fish and shellfish: An overview. Fish and Shellfish Immunology Reports 4, 100091. DOI: 10.1016/j.fsirep.2023.100091
Egerton S, Culloty S, Whooley J, Stanton C, Ross RP. 2018. The gut microbiota of marine fish. Frontiers in?Microbiology 9. DOI: 10.3389/fmicb.2018.00873
El-Kady AA, Magouz FI, Mahmoud SA, Abdel-Rahim MM. 2022. The effects of some commercial probiotics as water additive on water quality, fish performance, blood biochemical parameters, expression of growth and immune-related genes, and histology of nile tilapia (Oreochromis niloticus). Aquaculture 546 737249. DOI: 10.1016/j.aquaculture.2021.737249
El-Saadony MT, Alagawany M, Patra AK, Kar I, Tiwari R, Dawood MAO, Dhama K, Abdel-Latif HMR. 2021. The functionality of probiotics in aquaculture: an overview. Fish and Shellfish Immunology 117: 36–52. DOI: 10.1016/j.fsi.2021.07.007
Gobi N, Vaseeharan B, Chen JC, Rekha R, Vijayakumar S, Anjugam M, Iswarya A. 2018. Dietary supplementation of probiotic Bacillus licheniformis Dahb1 improves growth performance, mucus and serum immune parameters, antioxidant enzyme activity as well as resistance against Aeromonas hydrophila in tilapia Oreochromis mossambicus. Fish and Shellfish Immunology 74:501–508. DOI: 10.1016/j.fsi.2017.12.066
Gunduz M. 2005. Lactic Acid Production by Lactobacillus casei NRRL B-441 Immobilized In Chitosan Stabilized Ca-Alginate Beads. Izmir: Izmir Institute of Technology. Turkey
Hai CS, Yi XY, Qi JL, Wei C. 2023. Immunological effects of recombinant Lactobacillus casei expressing pilin MshB fused with cholera toxin B subunit adjuvant as an oral vaccine against Aeromonas veronii infection in crucian carp. Fish and Shellfish Immunology 139. DOI: 10.1016/j.fsi.2023.108934
Hai NV. 2015. Research findings from the use of probiotics in tilapia aquaculture: a review. Fish and Shellfish Immunology. 45 (2): 592–597.DOI: 10.1016/j. fsi.2015.05.026.
Haque MM, Hasan NA, Eltholth MM, Saha P, Mely SS, Rahman T, Murray FJ. 2021. Assessing the impacts of in-feed probiotic on the growth performance and health condition of pangasius (Pangasianodon hypophthalmus) in a farm trial. Aquaculture Reports. 20. DOI: 10.1016/j.aqrep.2021.100699.
Hardi EH, Nugroho NA, Rostika R, Choirum MM, Komsanah S, Rahayu W, Supriansyah, A, Saptiani G. 2022. Synbiotic application to enhance growth, immune system, and disease resistance toward bacterial infection in catfish (Clarias gariepinus). Aquaculture 549 737794. DOI: 10.1016/j.aquaculture.2021.737794
Hassan MA., Fathallah MA, Elzoghby MA. 2022. Influence of probiotics on water quality in intensified Litopenaeus vannamei ponds under minimum-water exchange. AMB Expr 12 22. DOI: 10.1186/s13568-022-01370-5
Hastuti, S., and Subandiyono, S., 2012. Hematological performances of African catfish (Clarias gariepinus) and media water qualities in culture system with bio-filtration pond. Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology 6(2): 1-5. DOI: 10.14710/ijfst.6.2.1-5
Heimbach JT. 2012. Generally Recognized as Safe (GRAS) Determination for the use of Lactobacillus casei FNCC 0049 as a food ingredient. Yakult Honsha Co., Ltd. Tokyo
Hendam BM, Mohammad BM, Moaheda EH, Ehab EH, Hien VD, Thian HC, El-Sayed HE. 2023. Effects of water additive probiotic, Pediococcus acidilactici on growth performance, feed utilization, hematology, gene expression and disease resistance against Aspergillus flavus of nile tilapia (Oreochromis niloticus). Animal Feed Science and Technology 303. DOI: 10.1016/j.anifeedsci.2023.115696.
Indriani F, Puspitasari I, Setyastuti TA, Santika A. 2020. Haematological parameters of Catfish (Clarias sp.) fed by immunostimulant added with Cr+3 - Yeast (Saccaromices cerevisiae) and Garlic. IOP Conf. Series: Earth and Environmental Science 441 (2020) 012115. DOI: 10.1088/1755-1315/441/1/012115
Kari AZ, Wee W, Mohamad SA, Che H, Hanif RMF, Irwan KM, Van DH, Wen GK, Seong WL. 2022. Role of phytobiotics in relieving the impacts of Aeromonas hydrophila infection on aquatic animals: A mini-review. Frontiers in Veterinary Science 9:1023784. DOI: 10.3389/fvets.2022.1023784.
Kusuma PSW, Hariani D, Mukti AT. 2022. Evaluation of Probiotic-fermented feed addition and laser-firing to accelerate mature brood stocks and seed productions of African catfish (Clarias gariepinus). Turkish Journal of Fisheries and Aquatic Sciences 22 19303. DOI: 10.4194/TRJFAS19303
Lawalata JH, Satiman U. 2015. Identification of lactic acid bacteria proteolytic isolated from an Indonesian traditional fermented fish sauce bakasang by amplified ribosomal DNA restriction analysis (ARDRA). International Journal of ChemTech Research; 8(12): 630-636.
Liu X, You Z, Luo W, Xiong J, Wang G. 2023. Blood cells and hematological parameters of Chiala Mountain Salamander, Batrachuperus karlschmidti (Urodela, Hynobiidae). PeerJ. DOI: 10.7717/peerj.15446.
Luo N, Wang L, Wang Z, Xiao B, Wang N, Yu X, Wu D, Song Z. 2022. Effects of dietary supplementation of duo-strain probiotics with post-spraying technology on growth performance, digestive enzyme, antioxidant capacity and intestinal microbiota of grass carp (Ctenopharyngodon idella). Aquaculture Reports 26 101301. DOI: 10.1016/j.aqrep.2022.101301
Malik A, Paiko AM, Adamu KM, Aliyu AA, Mohammed J. 2023. Role of prebiotic, probiotic and symbiotic diets on bacterial proliferation in feed and intestine of African catfish (Clarias gariepinus). Journal of Applied Sciences and Environmental Management 27(1). DOI:10.4314/jasem.v27i1.13
Michael M, Chikindas MC, Bren AB, Ermakov AM, Donnik IM, Belousova MM, Zolotukhin PV. 2021 Probiotic biomarkers and models upside down: from humans to animals. Veterinary Microbiology. 26, 109156. DOI: 10.1016/j.vetmic.2021.109156
Michael S, Abarike E, Cai J. 2019. A review on the probiotic effects on hematological parameters in fish. Journal of Fisheries Sciences.com 13(3): 25–31. DOI: 10.3390/antibiotics11020129
Mondal S, Mondal D, Mondal T, Malik J. 2022. Application of probiotic bacteria for the management of fish health in aquaculture. In: Dar, G.H., Bhat, R.A., Qadri, H., Al-Ghamdy, K.M., Hakeem, K.R. (Eds.), Bacterial fish diseases. Academic Press: 351–378. DOI: 10.1016/B978-0-323-85624-9.00024-5
Nugraha S, Ekasari J, Junior MZ Widanarni W. 2020. Digestive system and growth performance of African catfish larvae Clarias gariepinus, (Burchell, 1822) maintained with biofloc technology with the addition of Chlorella sp. Jurnal Iktiologi Indonesia 20(3): 297-306. DOI: 10.32491/jii.v20i3.535
Putra AN, Syamsunarno MB, Ningrum W, Jumyanah M. 2020. Effect of the administration of probiotic Bacillus NP5 in the rearing media on water quality, growth, and disease resistance of African catfish (Clarias gariepinus). Biodiversitas 21: 2566-2575. DOI: 10.13057/biodiv/d210629
Qiu Z, Qiyou X, Shenghao L, Dakua Z, Rongfei Z, Jianhua Z, Ting W. 2023. Effects of probiotics on the water quality, growth performance, immunity, digestion, and intestinal flora of giant freshwater prawn (Macrobrachium rosenbergii) in the biofloc culture system. Water 15 (6) 1211. DOI: 10.3390/w15061211
Ratnasari D. 2011. Dumbo catfish (Clarias gariepinus) rearing techniques at Biotech Agro, Jombang Regency, East Java Province. Field Work Practice Report. Faculty of Fisheries and Maritime Affairs, Airlangga University. Surabaya
Ray AK, Ringø E. 2014. The gastrointestinal tract of fish. in aquaculture nutrition: gut health, probiotics, and prebiotic. Wiley & Sons, Ltd, USA. DOI:10.1002/9781118897263
Safari R, Imanpour MR, Hoseinifar SH, Faheem M, Dadar M, Doan HV. 2022. Effects of dietary Lactobacillus casei on the immune, growth, antioxidant, and reproductive performances in male zebrafish (Danio rerio). Aquaculture Reports 25. DOI: 10.1016/j.aqrep.2022.101176
Serradell A, Torrecillas S, Soares F, Silva T, Montero D. 2023. Modelling the effect of prebiotics, probiotics and other functional additives on the growth, feed intake and feed conversion of European sea bass (Dicentrarchus labrax) juveniles. Aquaculture Reports 32 101729. DOI: 10.1016/j.aqrep.2023.101729
Sudrajat RH, Yuhana M, Widanarni W, Julie E, Afiff U. 2023. Dietary supplementation of Bacillus sp. NP5 and dayak onion simplicia powder Eleutherine bulbosa (Mill.) Urb. for the prevention of Aeromonas hydrophila in catfish Clarias sp. Jurnal Akuakultur Indonesia 22 2: 134-146. DOI: 10.19027/jai.22.2.134-146
Sumon TA, Hussain A, Sumon AA, Jang WJ, Abellan FG, Sharifuzzaman SM, Brown CL. 2022. Functionality and prophylactic role of probiotics in shellfish aquaculture. Aquaculture Reports 25, 101220. DOI: 10.1016/j.aqrep.2022.101220
Tabassum T, Mahamud SU, Acharjee TK, Hassan R, Snigdha TK, Islam TA, Khoiam U, Akter F, Azad R, Mahamud A, Ahmed GU, Rahman T. 2021. Probiotic supplementations improve growth, water quality, hematology, gut microbiota and intestinal morphology of nile tilapia. Aquaculture Reports 21. DOI: 10.1016/j.aqrep.2021.100972
Thurlow CM, Williams MA, Carrias A, Ran C, Newman M, Tweedie J, Allison E, Jescovitch LN, Wilson, AE, Terhune JS, Liles MR. 2019. Bacillus velezensis AP193 exerts probiotic effects in channel catfish (Ictalurus punctatus) and reduces aquaculture pond eutrophication. Aquaculture, 503: 347-35. DOI: 10.1016/j.aquaculture.2018.11.051
Wang J, Zhu Z, Li R, Wang X, Leng X, Chen, L. 2021. Impact of supplementary Lactobacillus casei K17 on growth and gut health of largemouth bass Micropterus salmoides. Aquaculture Reports 20 100734. DOI: 10.1016/j.aqrep.2021.100734
Wang YQ, Wang K, Xing PJ, Wang J. 2022. Dietary cinnamaldehyde and Bacillus subtilis improve growth performance, digestive enzyme activity, and antioxidant capability and shape intestinal microbiota in tongue sole Cynoglossus semilaevis. Aquaculture 543, 735798. DOI: 10.1016/j.aquaculture.2020.735798
Wedemeyer GA, Yasutake WT. 1977. Clinical methods for the assessement of the effect enviromental stress on fish health. U.S. Fish and Wildlife Service 89: 1-17
Yanuhar UDKW,Raharjo P, Caesar NR,Junirahma, NS. 2021. Hematology response of catfish (Clarias sp.) as an indicator of fish health in Tuban regency. IOP Conf. Series: Earth and Environmental Science 718. DOI:10.1088/1755-1315/718/1/012059
Yidi K, Min L, Jiaxin T, Linhui Z, Yuanhuan K, Lei Z, Guiqin W, Xiaofeng S. 2020. Effects of recombinant Lactobacillus casei on growth performance, immune response and disease resistance in crucian carp, Carassius auratus. Fish and Shellfish Immunology 99: 73-85. DOI: 10.1016/j.fsi.2020.02.008.
Yih TH, Chen SW, Hu SY. 2019. Improvements in the growth performance, immunity, disease resistance, and gut microbiota by the probiotic Rummeliibacillus stabekisii in nile tilapia (Oreochromis niloticus). Fish and Shellfish Immunology 92: 265–275. DOI: 10.1016/j.fsi.2019.06.027
Yukgehnaish K, Kumar P, Sivachandran P, Marimuthu K, Arshad A., Paray BA, Arockiaraj J. 2020. Gut microbiota metagenomics in aquaculture: factors influencing gut microbiome and its physiological role in fish. Reviews in Aquaculture: 1–25. DOI: 10.1111/raq.12416
Zhang D, Xu DH, Shoemaker CA, Beck BH. 2020. The severity of motile Aeromonas septicemia caused by virulent Aeromonas hydrophila in channel catfish is influenced by nutrients and microbes in water. Aquaculture 519. DOI: 10.1016/j.aquaculture.2019.734898.
Most read articles by the same author(s)
- JELENA ANGEL VISAKHADEVI, SAPTO ANDRIYONO, WORO HASTUTI SATYANTINI, NUR IZZATI MOHD NOH, Investigation of the bacterial diversity in fermented mangrove apple (Sonneratia caseolaris) fruit juice kombucha using DNA metabarcoding , Biodiversitas Journal of Biological Diversity: Vol. 25 No. 9 (2024)
- FATIMAH, NAZIL DWI RAHAYUNINGTYAS, ALIFAH NASTITI, DELA DWI ALAWIYAH, RICO RAMADHAN, ALMANDO GERALDI, JUNAIRIAH, Antibacterial and biosurfactant activity of endophytic bacteria isolated from mangrove plant in Lamongan, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 25 No. 7 (2024)
- BAYU PRANATA, ARADEA BUJANA KUSUMA, VERA SABARIAH, HYUN WOO KIM, SAPTO ANDRIYONO, Environmental DNA metabarcoding reveals biodiversity marine fish diversity of a small island at Manokwari District, West Papua, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 23 No. 11 (2022)
- SAPTO ANDRIYONO, NITA RUKMINASARI, ANDI ALIAH HIDAYANI, INDRA JUNAIDI ZAKARIA, MD. JOBAIDUL ALAM, HYUN-WOO KIM, Diversity of dinoflagellate cysts isolated from estuarine sediments of the Bengawan Solo and Brantas rivers, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 24 No. 2 (2023)
- SALAMUN, RIZKY DANANG SUSETYO, NI’MATUZAHROH, FATIMAH, ALMANDO GERALDI, AGUS SUPRIYANTO, TRI NURHARIYATI, FARAH AISYAH NAFIDIASTRI, NABILATUN NISA’, ENDARTO, Biosurfactant production of entomopathogenic Bacillus subtilis BK7.1, as potential biocontrol bacteria, isolated from Baluran National Park, East Java, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 24 No. 3 (2023)
- FARAH AISYAH NAFIDIASTRI, NATASYA THESSA LOLITA, HANA SHAFIYAH RIQINA, BELLA ZEZARI AURORA, ALMANDO GERALDI, TRI NURHARIYATI, AGUS SUPRIYANTO, NI’MATUZAHROH, FATIMAH, SALAMUN, The potential of indigenous Bacillus sp. BT3.1 as an antifungal biopesticide against the plant pathogen Fusarium oxysporum , Biodiversitas Journal of Biological Diversity: Vol. 25 No. 6 (2024)
- NURUL AINI, SRI PUJI ASTUTI WAHYUNINGSIH, FARAH NISA UZAKKY, CAROLINE BETZY, FATIMAH, SAPTO ANDRIYONO, Analysis of two strain probiotics on digestive enzymes, liver function and antimicrobial activity of catfish (Clarias gariepinus) treated with Aeromonas hydrophila , Biodiversitas Journal of Biological Diversity: Vol. 25 No. 11 (2024)