Identification of pigment profiles and antioxidant activity of Rhizophora mucronata mangrove leaves origin Lembeh, North Sulawesi, Indonesia
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Abstract. Rumengan AP, Mandiangan ES, Tanod WA, Paransa DSJ, Paruntu CP, Mantiri DMH. 2021. Identification of pigment profiles and antioxidant activity of Rhizophora mucronata mangrove leaves origin Lembeh, North Sulawesi, Indonesia. Biodiversitas 22: 2805-2816. Mangroves plants contain unique pigments which can serve both nutraceutical and pharmaceutical purpose. Therefore, this preliminary study aims to identify the pigment profiles of Rhizophora mucronata mangrove leaves (HPLC method) and to computationally evaluate the antioxidant mechanism of the pigments (PASS and STITCH analysis). Furthermore, it evaluated the antioxidant capacity of R. mucronata leaves extracts (DPPH method), and the pigment profiles detected using HPLC were chlorophyll a (68.61%), chlorophyll b (27.69%), lutein (29.94%), beta-carotene (14.05%), pheophytin a (8.72), violaxanthin (5.19%), and neoxanthin (3.65%). Beta-carotene, lutein, neoxanthin, and violaxanthin were predicted to have potential as antioxidants properties using PASS analysis. While neoxanthin and violaxanthin were predicted as free radical scavengers, beta-carotene was an Nrf-2 stimulant. The STITCH analysis showed that the pigments contained in the leaves interacted synergistically by activating as antioxidant enzymes and inhibiting the expression of oxidative stress proteins. The ethanol extract of R. mucronata leaves may be a potent antioxidant with an IC50 20.99 ± 0.33 µg/mL. Therefore, the pigment contained in R. mucronata leaves is a potential source of antioxidants.
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Adhikari A, Ray M, Das AK, Sur TK. 2016. Antidiabetic and antioxidant activity of Rhizophora mucronata leaves (Indian sundarban mangrove): An in vitro and in vivo study. Journal of Research in Ayurveda 37: 76–81. DOI: 10.4103/ayu.AYU
Agoramoorthy G, Chen FA, Venkatesalu V, Kuo DH, Shea, PC. 2008. Evaluation of antioxidant polyphenols from selected mangrove plants of India. Asian Journal of Chemistry 20: 1311–1322.
Aisiah S, Olga, Tanod WA, Salosso Y, Bambang, Riyadi PH. 2020. Computational analysis of ethyl acetate extract of Nauclea subdita (Korth.) Steud. leaves as peptidoglycan glycosyltransferase inhibitor in Aeromonas hydrophila. IOP Conference Series: Earth and Environmental Science 584: 012022. DOI: 10.1088/1755-1315/584/1/012022
Al-ali RM, Al-hilifi SAH, Al-Mossawi AEHJ. 2020. Extraction of lutein from some plant source in different conditions and application in food system. Plant Archives 20: 1679–1685.
Aliyu M, Abdullahi A, Ugya A. 2017. Antioxidant properties of selected poaceae species in Kano, Northern Nigeria. European Journal of Biomedical and Pharmaceutical Sciences 4: 577–585.
Aljaghthmi OH, Heba HM, Abu Zeid IM. 2017. Bioactive compounds extracted from mangrove plants (Avicennia marina and Rhizophora mucronata): an overview. Advances in Biological Research 11: 161–170. DOI: 10.1016/j.pathophys.2018.09.002
Analuddin K, Septiana A, Nasaruddin, Sabilu Y, Sharma S. 2019. Mangrove fruit bioprospecting: nutritional and antioxidant potential as a food source for coastal communities in the Rawa Aopa Watumohai National Park, Southeast Sulawesi, Indonesia. International Journal of Fruit Science 19: 423–436. DOI: 10.1080/15538362.2018.1555507
Andriani D, Revianti S, Prananingrum W. 2020. Identification of compounds isolated from a methanolic extract of Acanthus ilicifolius leaves and evaluation of their antifungal and antioxidant activity. Biodiversitas 21: 2521-2525. DOI: 10.13057/biodiv/d210625
Araki M, Kaku N, Harada M, Ando Y, Yamaguchi R, Shindo K. 2016. Production of auroxanthins from violaxanthin and 9-cis-violaxanthin by acidic treatment and the antioxidant activities of violaxanthin, 9-cis-violaxanthin, and auroxanthins. Journal of Agricultural and Food Chemistry 64: 9352–9355. DOI: 10.1021/acs.jafc.6b04506
Aruldass CA, Dufossé L, Ahmad WA. 2018. Current perspective of yellowish-orange pigments from microorganisms-a review. Journal of Cleaner Production 180: 168–182. DOI: 10.1016/j.jclepro.2018.01.093
Bandaranayake WM. 1998. Traditional and medicinal uses of mangroves. Mangroves and Salt Marshes 2: 133–148. DOI: 10.1023/A:1009988607044
Banerjee K, Chowdhury GR, Mitra A. 2017. Astaxanthin level of dominant mangrove floral species in Indian Sundarbans. MOJ Bioorganic & Organic Chemistry 1: 1–5. DOI: 10.15406/mojboc.2017.01.00008
Ben-dor A, Steiner M, Gheber L, Danilenko M, Dubi N, Linnewiel K, Zick A, Sharoni Y, Levy J. 2005. Carotenoids activate the antioxidant response element transcription system. Molecular Cancer Therapeutics 4: 177–187.
Berti AP, Düsman E, Mariucci RG, Lopes NB, Vicentini VEP. 2014. Antimutagenic and radioprotective activities of beta-carotene against the biological effects of iodine-131 radiopharmaceutical in wistar rats. Genetics and Molecular Research 13: 2248–2258. DOI: 10.4238/2014.March.31.5
Blaner WS, Brun PJ, Calderon RM, Golczak M. 2020. Retinol-binding protein 2 (RBP2): biology and pathobiology. Critical Reviews in Biochemistry and Molecular Biology 55: 197–218. DOI: 10.1080/10409238.2020.1768207
Blois M. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181: 1199–1200. DOI: 10.1038/1811199a0
Bogacz-Radomska L, Harasym J. 2018. ?-Carotene-properties and production methods. Food Quality and Safety 2: 69–74. DOI: 10.1093/fqsafe/fyy004
Bohn T, Walczyk T. 2004. Determination of chlorophyll in plant samples by liquid chromatography using zinc-phthalocyanine as an internal standard. Journal of Chromatography A 1024: 123–128. DOI: 10.1016/j.chroma.2003.10.067
Bowen-O’Connor CA, VanLeeuwen DM, Bettmann G, Sterling TM, Hilaire RSt. 2013. Variation in violaxanthin and lutein cycle components in two provenances of Acer grandidentatum L . exposed to contrasting light. Acta Physiologiae Plantarum 35: 541–548. DOI: 10.1007/s11738-012-1095-7
Chakraborty K, Raola VK. 2017. Two rare antioxidant and anti-inflammatory oleanenes from loop root Asiatic mangrove Rhizophora mucronata. Phytochemistry 135: 160–168. DOI: 10.1016/j.phytochem.2016.12.013
Chan SHH, Wu CWJ, Chang AYW, Hsu Ksen, Chan JYH. 2010. Transcriptional upregulation of brain-derived neurotrophic factor in rostral ventrolateral medulla by angiotensin II: significance in superoxide homeostasis and neural regulation of arterial pressure. Circulation Research 107: 1127–1139. DOI: 10.1161/CIRCRESAHA.110.225573
Chandrika UG, Jansz ER, Warnasuriya ND. 2005. Identification and HPLC quantification of carotenoids of the fruit pulp of Chrysophyllum roxburghii. Journal of the National Science Foundation of Sri Lanka 33: 93–98. DOI: 10.4038/jnsfsr.v33i2.2337
Chen P, Li L, Gao Y, Xie Z, Zhang Y, Pan Z, Tu Y, Wang H, Han Q, Hu X, Xin X. 2019. ? -carotene provides neuro protection after experimental traumatic brain injury via the Nrf2–ARE pathway. Journal of Integrative Neuroscience 18: 153–161. DOI: 10.31083/j.jin.2019.02.120
Croft H, Chen JM. 2017. Leaf pigment content. Elsevier Inc, Canada. DOI: 10.1016/B978-0-12-409548-9.10547-0
Cui T, Lai Y, Janicki JS, Wang X. 2016. Nuclear factor erythroid-2 related factor 2 (Nrf2)-mediated protein quality control in cardiomyocytes. Frontiers in Bioscience 21: 192–202.
Dahdouh-Guebas F, Mathenge C, Kairo JG, Koedam N. 2000. Utilization of mangrove wood products around Mida Creek “Kenya” amongst subsistence and commercial users. Economic Botany 54: 513–527. DOI: 10.1007/BF02866549
Dall’Osto L, Cazzaniga S, North H, Marion-Poll A, Bassia R. 2007. The arabidopsis aba4-1 mutant reveals a specific function for neoxanthin in protection against photooxidative stress. Plant Cell 19: 1048–1064. DOI: 10.1105/tpc.106.049114
Dasgupta N, Nandy P, Sengupta C, Das S. 2012. Protein and enzymes regulations towards salt tolerance of some Indian mangroves in relation to adaptation. Trees - Structure and Function 26: 377–391. DOI: 10.1007/s00468-011-0599-x
Dewanto DK, Finarti F, Hermawan R, Ndobe S, Riyadi PH, Tanod WA. 2019. Antioxidant activity of soft coral extract from Palu Bay, Central Sulawesi, Indonesia. Jurnal Pascapanen Dan Bioteknologi Kelautan Dan Perikanan 14: 163–178. DOI: 10.15578/jpbkp.v14i2.583 [Indonesian]
Dewanto DK, Hermawan R, Muliadin, Riyadi PH, Aisiah S, Tanod WA. 2021. GC-MS profile of Rhizophora apiculata leaf extract from the coast of Tomini bay, Central Sulawesi with antibacterial and antioxidant activity. Jurnal Kelautan: Indonesian Journal of Marine Science and Technology 14: 30–42. [Indonesian]
Dewanto DK, Tanod WA, Finarti F, Renol R. 2018. Screening of Antiradical Activity From Some Central Sulawesi Mangroves. Pharmaciana 8: 155–168. DOI: 10.12928/pharmaciana.v8i1.8187
Dou Z, Cui L, Li J, Zhu Y, Gao C, Pan X, Lei Y, Zhang M, Zhao X, Li W. 2018. Hyperspectral estimation of the chlorophyll content in short-term and long-term restorations of Mangrove in Quanzhou Bay Estuary, China. Sustainability 10: 1–15. DOI: 10.3390/su10041127
Durga Devi M, Banu N. 2015. Study of antioxidant activity of chlorophyll from some medicinal plants. Indian Journal of Research 4: 6–8.
Eijckelhoff C, Dekker JP. 1997. A routine method to determine the chlorophyll a, pheophytin a and B-carotene contents of isolated Photosystem II reaction center complexes. Photosynthesis Research 52: 69–73. DOI: 10.1023/A:1005834006985
Faiqoh M, Utami TFY, Pertiwi Y. 2020. Antioxidant assay for stick balm preparation of Rhizophora mucronata leaf extract using DPPH method. Jurnal Ilmiah Jophus?: Journal of Pharmacy UMUS 2: 51–58. [Indonesian]
Falqueto A, Silva DM, Fontes RV. 2008. Photosynthetic performance of mangroves Rhizophora mangle and Laguncularia racemosa under field conditions. Revista Árvore 32: 577–582. DOI: 10.1590/S0100-67622008000300018
Filimonov DA, Lagunin AA, Gloriozova TA, Rudik AV, Druzhilovskii DS, Pogodin PV, Poroikov VV. 2014. Prediction of the biological activity spectra of organic compounds using the pass online web resource. Chemistry of Heterocyclic Compounds 50: 444–457. DOI: 10.1007/s10593-014-1496-1
Flores-de-Santiago F, Kovacs JM, Wang J, Flores-Verdugo F, Zhang C, González-Farías F. 2016. Examining the influence of seasonality, condition, and species composition on mangrove leaf pigment contents and laboratory based spectroscopy data. Remote Sensing 8: 226–246. DOI: 10.3390/rs8030226
Fogelgren B, Polgár N, Szauter KM, Újfaludi Z, Laczkó R, Fong KSK, Csiszar K. 2005. Cellular fibronectin binds to lysyl oxidase with high affinity and is critical for its proteolytic activation. Journal of Biological Chemistry 280: 24690–24697. DOI: 10.1074/jbc.M412979200
Gao Z, Li YH. 2012. Antioxidant stress and anti-inflammation of PPAR ? on warm hepatic ischemia-reperfusion injury. PPAR Research 2012: 1–8. DOI: 10.1155/2012/738785
Giossi CE, Cartaxana P, Cruz S. 2020. Photoprotective role of neoxanthin in plants and algae. Molecules 25: 4617–4633. DOI: 10.3390/molecules25204617
Gupta P, Sreelakshmi Y, Sharma R. 2015. A rapid and sensitive method for determination of carotenoids in plant tissues by high performance liquid chromatography. Plant Methods 11: 1–12. DOI: 10.1186/s13007-015-0051-0
Hegazi MM, Perez-ruzafa A, Almela L, Candela ME. 1998. Separation and identification of chlorophylls and carotenoids from Caulerpa prolifera, Jania rubens and Padina pavonica by reversed- phase high-performance liquid chromatography. Journal of Chromatography A 829: 153–159.
Hoekstra M, Sorci-Thomas M. 2017. Rediscovering scavenger receptor type BI: surprising new roles for the HDL receptor. Current Opinion in Lipidology 28: 255–260. DOI: 10.1097/MOL.0000000000000413
Hsu CY, Chao PY, Hu SP, Yang CM. 2013. The antioxidant and free radical scavenging activities of chlorophylls and pheophytins. Food and Nutrition Sciences 4: 1–8. DOI: 10.4236/fns.2013.48a001
Imdadul H, Wirakarnain S, Koshy P, Arash R, Shariff HABM, Mat TR. 2011. Valuable antioxidant and antimicrobial extracts from Rhizophora mucronata of Asiatic mangrove forests. Research Journal of Biotechnology 6: 10–14.
?nanç AL. 2011. Chlorophyll: structural properties, health benefits and its occurrence in virgin olive oils. Akademik G?da 9: 26–32.
Ingkasupart P, Manochai B, Song WT, Hong JH. 2015. Antioxidant activities and lutein content of 11 marigold cultivars (Tagetes spp.) grown in Thailand. Food Science and Technology 35: 380–385. DOI: 10.1590/1678-457X.6663
Isnindar, Wahyuono S, Widyarini S, Yuswanto. 2016. Determination of antioxidant activities of buas-buas leaves (Premna serratifolia L.) using DPPH (2,2-diphenyl-1- picrylhydrazyl) method. Traditional Medicine Journal 21: 111–115. DOI: 10.22146/tradmedj.17292
Jariyah, Widjanarko SB, Yunianta, Estiasih T, Sopade PA. 2014. Pasting properties mixtures of mangrove fruit flour (Sonneratia caseolaris) and starches. International Food Research Journal 21: 2161–2167.
Jeffrey SW, Mantoura RFC, Wright SW. 1997. Phytoplankton pigments in oceanography: guidelines to modern methods. UNESCO Publishing, Paris. www.linkinghub.elsevier.com/retrieve/pii/S0031942297008108
Jithesh MN, Prashanth SR, Sivaprakash KR, Parida AK. 2006. Antioxidative response mechanisms in halophytes. Journal of Genetics 85: 237–254.
Johnson E, Etim E, Archibong E. 2017. Isolation and anti-oxidant potentials of parahydroxybenzaldehyde from the methanol leaf extract of Aspilia africana (Pers.) C.D. Adams (Asteraceae). Nigerian Journal of Pharmaceutical and Applied Science Research 6: 26–32. www.nijophasr.com/articles/volume6/issue2/article109.pdf
Kamoshita M, Toda E, Osada H, Narimatsu T, Kobayashi S, Tsubota K, Ozawa Y. 2016. Lutein acts via multiple antioxidant pathways in the photo-stressed retina. Scientific Reports 6: 1–10. DOI: 10.1038/srep30226
Kardiman, Ridhwan M, Armi. 2017. Lindur fruit (Bruguiera gymnorrhiza) as food for the Acehnese community. Serambi Saintia 5: 51–55. [Indonesian]
Kepel TL, Suryono DD, Ati RNA, Salim HL, Hutahaean AA. (2017). Important value and economic value estimation of carbon storage of mangrove vegetation in Kema, North Sulawesi. Jurnal Kelautan Nasional 12: 19–26. DOI: 10.15578/jkn.v12i1.6170 [Indonesian]
Kuhn M, Von Mering C, Campillos M, Jensen LJ, Bork P. 2008. STITCH: Interaction networks of chemicals and proteins. Nucleic Acids Research 36: 684–688. DOI: 10.1093/nar/gkm795
Kurniawan JM, Yusuf MM, Heriyanto, Panintingjati TH, Brotosudarmo. 2020. Literature review on the potential of lutein from local marigold flowers as a health supplement. Media Penelitian Dan Pengembangan Kesehatan 30: 147–162. DOI: 10.22435/mpk.v30i2.2874 [Indonesian]
Kusbandari A, Susanti H. 2017. Beta carotene content and free radical scavenging activity of cantaloupe (Cucumis melo var. Cantalupensis L.) extract against DPPH (1,1-diphenyl-2-picrylhydrazyl) using UV-visible spectrophotometry method. Jurnal Farmasi Sains Dan Komunitas 14: 37–42. DOI: 10.24071/jpsc.141562 [Indonesian]
Kusmita L, Puspitaningrum I, Limantara L. 2015. Identification, isolation and antioxidant activity of pheophytin from green tea (Camellia Sinensis (L.) Kuntze). Procedia Chemistry 14: 232–238. DOI: 10.1016/j.proche.2015.03.033
Landrum JT, Bone RA. 2001. Lutein, zeaxanthin, and the macular pigment. Archives of Biochemistry and Biophysics 385: 28–40. DOI: 10.1006/abbi.2000.2171
Lee B, Cao R, Choi YS, Cho HY, Rhee AD, Hah CK, Hoyt KR, Obrietan K. 2009. The CREB/CRE transcriptional pathway: protection against oxidative stress-mediated neuronal cell death. Journal of Neurochemistry 108: 1251–1265. DOI: 10.1111/j.1471-4159.2008.05864.x
Lietz G, Lange J, Rimbach G. 2010. Molecular and dietary regulation of ?,?-carotene 15,15?-monooxygenase 1 (BCMO1). Archives of Biochemistry and Biophysics 502: 8–16. DOI: 10.1016/j.abb.2010.06.032
Lietz G, Oxley A, Boesch-Saadatmandi C, Kobayashi D. 2012. Importance of ?,?-carotene 15,15?-monooxygenase 1 (BCMO1) and ?,?-carotene 9?,10?-dioxygenase 2 (BCDO2) in nutrition and health. Molecular Nutrition and Food Research 56: 241–250. DOI: 10.1002/mnfr.201100387
Limantara L, Heriyanto. 2012. Pigment composition and content of fookosantin brown seaweed from madura waters with high performance liquid chromatography. Ilmu Kelautan: Indonesian Journal of Marine Sciences 15: 23–32. DOI: 10.14710/ik.ijms.15.1.23-32 [Indonesian]
Liu Y. 2012. Spectral response to varying levels of leaf pigments collected from a degraded mangrove forest. Journal of Applied Remote Sensing 6: 063501-1-063501–063514. DOI: 10.1117/1.jrs.6.063501
Lung JKS, Destiani DP. 2014. Antioxidant activity test of vitamins A, C, E with the DPPH method. Farmaka, 14: 1–10. [Indonesian]
Ma Q. 2013. Role of Nrf2 in oxidative stress and toxicity. Annual Review of Pharmacology and Toxicology 53: 401–426. DOI: 10.1146/annurev-pharmtox-011112-140320.Role
Mangunsong S, Assiddiqy R, Sari EP, Marpaung PN, Sari RA. 2019. Determination of ?-carotene in carrots (Daucus carota) by high performance liquid chromatography (U-HPLC). AcTion: Aceh Nutrition Journal 4: 36–41. DOI: 10.30867/action.v4i1.151 [Indonesian]
Mapari SAS, Nielsen KF, Larsen TO, Frisvad JC, Meyer AS, Thrane, U. 2005. Exploring fungal biodiversity for the production of water-soluble pigments as potential natural food colorants. Current Opinion in Biotechnology 16: 231–238. DOI: 10.1016/j.copbio.2005.03.004
Melendez-Mart?nez AJ, Britton G, Vicario IM, Heredia FJ. 2008. The complex carotenoid pattern of orange juices from concentrate. Food Chemistry 109: 546–553. DOI: 10.1016/j.foodchem.2008.01.003
Moran E, Ding L, Wang Z, Cheng R, Chen Q, Moore R, Takahashi Y, Ma JX. 2014. Protective and antioxidant effects of PPAR? the ischemic retina. Investigative Ophthalmology and Visual Science 55: 4568–4576. DOI: 10.1167/iovs.13-13127
Mueller L, Boehm V. 2011. Antioxidant activity of ?-carotene compounds in different in vitro assays. Molecules 16: 1055–1069. DOI: 10.3390/molecules16021055
Najihudin A, Chaerunisaa A, Subarnas A. 2017. Antioxidant activity of trengguli (Cassia Fistula L) bark extract and fraction using the DPPH method. Indonesian Journal of Pharmaceutical Science and Technology 4: 70–78. DOI: 10.15416/ijpst.v4i2.12354
Ngginak J, Mangibulude JC, Rondonuwu FS. 2017. The identification of carotenoids and testing of carotenoid antioxidants from sand lobster (Panulirus homarus) egg extract. Ilmu Kelautan 2: 155–160. DOI: 10.14710/ik.ijms.22.3.155-160
Oelkrug R, Goetze N, Meyer CW, Jastroch M. 2014. Antioxidant properties of UCP1 are evolutionarily conserved in mammals and buffer mitochondrial reactive oxygen species. Free Radical Biology and Medicine 77: 210–216. DOI: 10.1016/j.freeradbiomed.2014.09.004
Oke JM, Hamburger O. 2002. Screening of some Nigerian medicinal plants for antioxidant activity using 2,2, diphenyl-picryl-hydrazyl radical. African Journal of Biomedical Research 5: 77–79.
Paolini M, Abdel-Rahman SZ, Sapone A, Pedulli GF, Perocco P, Cantelli-Forti G, Legator MS. 2003. ?-carotene: a cancer chemopreventive agent or a co-carcinogen? Mutation Research - Reviews in Mutation Research 543: 195–200. DOI: 10.1016/S1383-5742(03)00002-4
Paransa DSJ, Kemer K, Rumengan AP, Mantiri DM. 2014. Pigment type analysis and antibacterial activity assay of xanthophyll pigment extract on brown algae Sargassum polycystum (C. Agardh). Jurnal LPPM Bidang Sains Dan Teknologi 1: 90–96. [Indonesian]
Pareek S, Sagar NA, Sharma S, Kumar V, Agarwal T, González-Aguilar GA, Yahia EM. 2017. Chlorophylls: chemistry and biological functions. In: Yahia EM (eds) Fruit and Vegetable Phytochemicals: Chemistry and Human Health: Second Edition (Vol. 1, Second Edition). John Wiley & Sons Ltd, New Jersey. DOI: 10.1002/9781119158042.ch14
Pérez-gálvez A, Viera I, Roca M. 2020. Carotenoids and chlorophylls as antioxidants. Antioxidants 9: 1–39. DOI: 10.3390/antiox9060505
Perreau F, Frey A, Effroy-Cuzzi D, Savane P, Berger A, Gissot L, Marion-Poll A. 2020. Abscisic acid-deficient4 has an essential function in both cis-violaxanthin and cis-neoxanthin synthesis. Plant Physiology 184: 1303–1316. DOI: 10.1104/pp.20.00947
Prabhu KH, Bhute AS. 2015. Plant based dyes and mordant: a review. Journal of Natural Products and Plant Resources 2: 649–664.
Pratiwi D, Wahdaningsih S, Isnindar. 2013. The test of antioxidant activity from bawang mekah leaves (Eleutherine americana Merr.) using DPPH (2,2- diphenyl-1-picrylhydrazyl) method. Traditional Medicine Journal 18: 9–16. DOI: 10.1007/s13398-014-0173-7.2
Prestigiacomo V, Suter-Dick L. 2018. Nrf2 protects stellate cells from Smad-dependent cell activation. PLoS ONE 13: 1–18. DOI: 10.1371/journal.pone.0201044
Pringgenies D, Pratiwi AHD, Yudiati E, Azizah R, Susilo ES. 2017. Biopigment tracing of mangrove Rhizophora mucronata leaf and bark waste and its application in batik fabrics with various fixation methods. In: Dhramawan HA (eds.) Proceedings Book The 7th Basic Science International Conference. Universitas Brawijaya, Malang, 7-8 March 2017.
Pringgenies D, Ridlo A, Dewi LF, Djunaedi A. 2018. The commercial value of mangrove-based pigments as natural dye for batik textiles delianis. IntechOpen (Vol. 32). IntechOpen, London. www.intechopen.com/books/trends-in-telecommunications-technologies/gps-total-electron-content-tec- prediction-at-ionosphere-layer-over-the-equatorial-region%0AInTec
Puspitasar YE, Hartiati AM, Suprayitno E. 2012. The potency of Rhizophora mucronata leaf extract as antidiarrhea. Journal of Applied Sciences Research 8: 1180–1185.
Radu GL, Litescu SC, Albu C, Teodor E, Truica G. 2012. Beta-carotene and lycopene determination in new enriched bakery products by HPLC-DAD method. Romanian Biotechnological Letters 17: 7005–7012.
Ratnasari N, Walters M, Tsopmo A. 2017. Antioxidant and lipoxygenase activities of polyphenol extracts from oat brans treated with polysaccharide degrading enzymes. Heliyon 3: e00351. DOI: 10.1016/j.heliyon.2017.e00351
Ravikumar S, Gnanadesigan M. 2012. Hepatoprotective and antioxidant properties of Rhizophora mucronata mangrove plant in CCl 4 intoxicated rats. Journal of Experimental and Clinical Medicine 4: 66–72. DOI: 10.1016/j.jecm.2011.11.012
Ravindran C, Naveenan T, Varatharajan GR, Rajasabapathy R, Meena RM. 2012. Antioxidants in mangrove plants and endophytic fungal associations. Botanica Marina 55: 269–279. DOI: 10.1515/bot-2011-0095
Revathi P, Jeyaseelan Senthinath T, Thirumalaikolundusubramanian P, Prabhu N. 2014. An overview of antidiabetic profile of mangrove plants. International Journal of Pharmacy and Pharmaceutical Sciences 6: 1–5.
Ridlo A, Pramesti R, Koesoemadji K, Supriyantini E, Soenardjo N. 2017. Antioxidant activity of Rhizophora mucronata mangrove leaf extract. Buletin Oseanografi Marina 6: 110–116. DOI: 10.14710/buloma.v6i2.16555 [Indonesian]
Riyadi PH, Romadhon, Anggo AD, Suharto S, Tanod WA, Aryani A. 2021. Anti-inflammatory potential from tilapia (Oreochromis niloticus) viscera hydrolysate with bioinformatics analysis (prediction of activity spectra for substances – PASS). IOP Conference Series: Earth and Environmental Science 750: 012044. DOI: 10.1088/1755-1315/750/1/012044
Riyadi PH, Tanod WA, Wahyudi D, Susanto E, Fahmi AS, Aisiah S. 2020. Potential of tilapia (Oreochromis niloticus) viscera bioactive peptides as antiviral for SARS-CoV-2 (COVID 19). IOP Conference Series: Earth and Environmental Science 584: 012004. DOI: 10.1088/1755-1315/584/1/012004
Riyadi PH, Wahyudi D, Tanod WA. 2019. Effects of dichloromethane Sarcophyton spp. extract on the lipopolysaccharide-induced expression of nuclear factor-kappa B and inducible nitric oxide synthase in mice. Veterinary World 12: 1897–1902. DOI: 10.14202/vetworld.2019.1897-1902
Rohini RM, Das AK. 2009. A comparative evaluation of analgesic and anti-inflammatory activities of Rhizophora mucronata bark extracts. Pharmacologyonline 1: 780–791.
Rout P, Basak UC. 2014. Antioxidant properties in leaf and root extracts of some medicinally important mangrove species of Odisha coast. American Journal of Pharmtech Research 4: 605–617.
Ruban AV, Pascal AA, Robert B, Horton P. 2001. Configuration and dynamics of xanthophylls in light-harvesting antennae of higher plants. Journal of Biological Chemistry 276: 24862–24870. DOI: 10.1074/jbc.M103263200
Rumengan AP, Mantiri DMH, Rompas R, Hutahaean A, Kepel TL, Paruntu CP, Kepel RC, Gerung GS. 2018. Carbon stock assessment of mangrove ecosystem in Totok bay, Southeast Minahasa regency, North Sulawesi, Indonesia. AACL Bioflux 11: 1280–1288.
?ahin S, Aybast?er Ö, Dawbaa S, Karkar B, Çakmak T. 2020. Study of the ability of lutein and neoxanthin as standards and in the extract of Chlamydomonas reinhardtii to prevent oxidatively induced DNA base damage using ultrasensitive GC–MS/MS analysis. Chromatographia 83: 919–926. DOI: 10.1007/s10337-020-03918-8
Saptiani G, Asikin AR, Ardhani F, Hardi EH. 2018. Mangrove plants species from Delta Mahakam, Indonesia with antimicrobial potency. Biodiversitas 19: 516-521. DOI: 10.13057/biodiv/d190220
Sarker U, Oba S. 2020. Leaf pigmentation, its profiles and radical scavenging activity in selected Amaranthus tricolor leafy vegetables. Scientific Reports 10: 1–10. DOI: 10.1038/s41598-020-66376-0
Sasamoto H, Hayatsu M, Suzuki S. 2020. High allelopathic activity of carotenoid-accumulating callus of a halophilic mangrove plant, Avicennia alba: protoplast co-culture method with digital image analysis. Journal of Plant Studies 9: 1–12. DOI: 10.5539/jps.v9n1p1
Sasmito BB, Puspitasari YE, Hardoko H. 2016. Antidiabetic and antioxidant activities of tannin extract of Rhizophora mucronata leaves. Journal of Chemical and Pharmaceutical Research 8: 143–148.
Shabalina IG, Petrovic N, Kramarova TV, Hoeks J, Cannon B, Nedergaard J. 2006. UCP1 and defense against oxidative stress: 4-hydroxy-2-nonenal effects on brown fat mitochondria are uncoupling protein 1-independent. Journal of Biological Chemistry 281: 13882–13893. DOI: 10.1074/jbc.M601387200
Soran H, Schofield JD, Durrington PN. 2015. Antioxidant properties of HDL. Frontiers in Pharmacology 6: 1–6. DOI: 10.3389/fphar.2015.00222
Strobel M, Tinz J, Biesalski HK. 2007. The importance of ?-carotene as a source of vitamin A with special regard to pregnant and breastfeeding women. European Journal of Nutrition 46: 1–20. DOI: 10.1007/s00394-007-1001-z
Suganthy N, Pandima Devi K. 2016. In vitro antioxidant and anti-cholinesterase activities of Rhizophora mucronata. Pharmaceutical Biology 54: 118–129. DOI: 10.3109/13880209.2015.1017886
Taiz L, Zeiger E, Møller IM, Murphy A. 2006. Plant Physiology (Fifth). Sinauer Associates Inc., Massachusetts. www.sinauer.com/media/wysiwyg/tocs/PlantPhysiology5.pdf
Tanod WA, Dewanto DK, Ndobe S, Riyadi PH, Putra MY. 2019. Screening of antibacterial and antioxidant activity of soft corals Sinularia sp. and Sarcophyton sp. from Palu bay, Central Sulawesi, Indonesia. Squalen: Bulletin of Marine and Fisheries Postharvest and Biotechnology 14: 73–83. DOI: 10.15578/squalen.v14i2.394
Tanod WA, Yanuhar U, Maftuch, Wahyudi D, Risjani Y. 2019. DPPH scavenging property of bioactives from soft corals origin Palu bay, Central Sulawesi, Indonesia. IOP Conference Series: Earth and Environmental Science 236: 012121. DOI: 10.1088/1755-1315/236/1/012121
Thatoi HN, Patra JK, Das SK. 2014. Free radical scavenging and antioxidant potential of mangrove plants: a review. Acta Physiologiae Plantarum 36: 561–579. DOI: 10.1007/s11738-013-1438-z
Tidore F, Rumengan AP, Sondak CFA, Mangindaan REP, Runtuwene HCC, Pratasik SB. 2018. Estimation of carbon (C) content in mangrove leaves from Lansa village, Wori sub- district, North Minahasa district. Jurnal Pesisir Dan Laut Tropis 2: 53–58. DOI: 10.35800/jplt.6.2.2018.21529 [Indonesian]
Wang F, Huang L, Gao B, Zhang C. 2018. Optimum production conditions, purification, identification, and antioxidant activity of violaxanthin from Microalga Eustigmatos cf. polyphem (Eustigmatophyceae). Marine Drugs 16: 190–203. DOI: 10.3390/md16060190
Widyowati H, Ulfah M, Sumantri. 2014. Antioxidant activity test of the herba alfalfa ethanolic extract (Medicago sativa L.) with the DPPH method (1,1-Diphenyl-2Picrylhidrazyl). Jurnal Ilmu Farmasi Dan Farmasi Klinik 11: 25–33. www.publikasiilmiah.unwahas.ac.id/index.php/Farmasi/article/view/1285/1390 [Indonesian]
Wu CL, Chen SD, Yin JH, Hwang CS, Yang DI. 2016. Nuclear factor-kappaB-dependent sestrin2 induction mediates the antioxidant effects of BDNF against mitochondrial inhibition in rat cortical neurons. Molecular Neurobiology 53: 4126–4142. DOI: 10.1007/s12035-015-9357-1
Wu CL, Yin JH, Hwang CS, Chen SD, Yang DY, Yang DI. 2012. C-Jun-dependent sulfiredoxin induction mediates BDNF protection against mitochondrial inhibition in rat cortical neurons. Neurobiology of Disease 46: 450–462. DOI: 10.1016/j.nbd.2012.02.010
Yabluchanskiy A, Ma Y, Iyer RP, Hall ME, Lindsey ML. 2013. Matrix metalloproteinase-9: many shades of function in cardiovascular disease. Physiology 28: 391–403. DOI: 10.1152/physiol.00029.2013
Zapata M, Rodríguez F, Garrido JL. 2000. Separation of chlorophylls and carotenoids from marine phytoplankton: a new HPLC method using a reversed phase C8 column and pyridine-containing mobile phases. Marine Ecology Progress Series 195: 29–45. DOI: 10.3354/meps195029
Zhang T, Wang Z, Wang X, Sun W, Cui X, Li R, Li G. 2019. Effects of vitamin A on antioxidant functions, immune functions and production performance in male sika deer (Cervus nippon) during the first antler growth period. Italian Journal of Animal Science 18: 98–104. DOI: 10.1080/1828051X.2018.1456978