Characterization of flavonoids from candidate striga grass in controlling diet legumes Cicer arietinum and Vigna radiata




Abstract. Akumu AA, Nyambaka H, Kuate SP, Torto B.2018. Characterization of flavonoids from candidate striga grass in controlling diet legumes Cicer arietinum and Vigna radiata. Biofarmasi J Nat Prod Biochem 16: 83-98. In Africa, intercropping of some legumes with cereal plants has been noticed to generate a remarkable reduction of Striga invasion and enhanced cereal crop production. Desmodium uncinatum administers Striga invasion in intercrop with cereals through an allelopathic technique, including post-sprouting accretion inhibitors emanating from the roots. Having the potential to be a new technique for grass administering, Allelopathy is a natural and environmentally friendly method. A standardized profiling technique based on Reverse Phase-High Performance Liquid Chromatography (RP-HPLC) and Liquid Chromatography coupled with Mass Spectrometry (LC/MS) was utilized to determine flavonoids in extracts of chickpea (Cicer arietinum) and mung bean (Vigna radiata), potential trap plants for Striga grass. Nine flavonoids involving mono-and diglycosyl derivatives of fisetin, baicalein, rhamnetin, isorhamnetin, formononetin, quercetin, isosakuranetin, and sakuranetin were temporarily distinguished. The obtained phenolics were available at concentrations higher than 0.001% of the dry materials. Many of these phenolic mixtures have been claimed to possess human health advantages. These glycosylated flavones are noted for the first time in these two species. The comprehensive analysis of the polar secondary metabolites in these leguminous plants was useful for comprehending their inhibitory chemistry and proposed biosynthesis by C-glucoside characteristics. All the segregated and described mixtures in the diet legume C. arietinum were O-glucosylated. At the same time, Food legume V. radiata should be observed further to see if it can attain Desmodium’s allelopathic ability since it possesses inhibitory chemistry and proposed biosynthesis by C-glucosylation.


Ahmed S. 2007. Pharmacognosy, Introduction of plant constituents and
their tests. Faculty of Pharmacy, Jamia Hamdard Nagar, New Delhi.

Andersen OM, Markham KR. 2006. Flavonoids, Chemistry, Biochemistry
and Applications. CRC Press, USA.

Anon. 2012. Phenol-Explorer database on polyphenol content in foods, 16
November 2012 retrieved from:’ table.

Esilaba AO. 2006. Options for Striga management in Kenya. KARI
Technical note, No. 19, March 2006.

Guchu SM. 2007. Studies on Desmodium species for the Allelochemicals
involved in Striga suppression. University of Nairobi 87-98
[Unpublished Thesis].

Hooper AM, Hassanali A, Chamberlain K, Khan Z, Pickett JA. 2009. New
genetic opportunities for legume intercrops for controlling Striga spp.
parasitic weeds. Pest Manag Sci 65 (5): 546-552.

Hooper AM, Tsanuo MK, Chamberlain K, Tittcomb K, Scholes J,
Hassanali A, Khan ZR, Pickett JA. 2010. Isoschaftoside, a Cglycosylflavonoid from Desmodium uncinatum root exudate, is an
allelochemical against the development of Striga. Phytochemistry 71
(8-9): 904-908.

Khan ZR, Midega CAO, Bruce TJA, Hooper AM, Pickett JA. 2010.
Exploiting phytochemicals for developing a ‘push-pull’ crop
protection strategy for cereal farmers in Africa. J Exp Bot 61 (51):

Kureh I, Chikoye D, Emechebe AM, Hussaini MA, Kormawa P, Schulz S,
Ellis-Jones J, Franke L, Odunze AC. 2003. Reduction of Striga
hermonthica parasitism on maize using host plant resistance, n
fertilization and rotation with legume trap crop. Afr Crop Sci Soc 6:

Othira JO, Deng AL, Onek LA, Kemey J, Omolo EO. 2008. Potential
application of Hyptis spicigera for biological control of Striga
hermonthica infestation. African J Agric Res 3 (10): 747-755.

Pickett JA, Hamilton ML, Hooper AM, Khan ZR, Midega CAO. 2010.
Companion cropping to manage parasitic plants. Ann Rev
Phytopathol 48: 161-77.

Pickett JA, Khan ZR, Hassanali A, Hooper AM. 2007. Chemicals
involved in post-germination inhibition of Striga by Desmodium:
Opportunities for utilizing the associated allelochemical traits. In:
Ejeta G, Gressel J (eds.). Integrating New Technologies for Striga
control: Towards ending the Witch-Hunt. World Scientific Publishing
Company Pte. Ltd., Singapore.

Pickett JA. 2011. Innovations for sustainable intensification of agricultural
production: knowledge-intensive routes to doubling yields and
reducing inputs. Millennium Address, New Delhi, India

Raymond EM, Errol GL, Christopher JS, Xiu-Sheng M, Xiaoming Z,
Chris DM. 2006A comparison of flavonoid glycosides by
electrospray tandem mass spectrometry. Intl J Mass Spectrom 248 (1-
2): 61-85.

Rich PJ, Ejeta G. 2008. Towards effective resistance to Striga in Africa
maize. Plant Signal Behav 3 (9): 618-621.

Tsai JC, Huang GJ, Chiu TH, Huang SS, Huang SC, Huang TH, LaiSC,
Lee CY. 2011. Antioxidant activities of phenolic components from
various plants of Desmodium species. Afr J Pharm Pharmacol 5 (4):

Tsanuo MK, Hassanali A, Hopper AM, Khan ZR, Kaberia F, Pickett JA,
Wadhams LJ. 2003. Isoflavonones from the allelopathic aqueous root
exudates of Desmodium uncinatum. Phytochemistry 64: 265-273.

Zaji B, Majd A. 2011. Allelopathic potential of Canola (Brassica napus
L.) Residues on weed suppression and yield response of maize (Zea
mays L.). International conference on chemical. Ecol Environ Sci