Enhancing germination performance and vigor of lettuce and napa cabbage seeds through simple seed priming techniques under controlled conditions
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Abstract. Ria RP, Rizar FF, Budainta D, Sefrila M, Gustiar F. 2026. Enhancing germination performance and vigor of lettuce and napa cabbage seeds through simple seed priming techniques under controlled conditions. Asian J Agric 10: g100175. https://doi.org/10.13057/asianjagric/g100175. Introduced leafy vegetable seeds marketed for urban farming are often subjected to prolonged storage and suboptimal handling before use, which may reduce seed vigor, delay germination, and lead to non-uniform seedling emergence. This study evaluated simple seed priming treatments to improve germination performance in four commercial leafy vegetable seed types: butterhead lettuce, red romaine lettuce, green lettuce, and napa cabbage. The experiment was conducted in September 2025 at the Seed Technology Laboratory, Universitas Sriwijaya, Indonesia, using a factorial completely randomized design. Seeds were subjected to four treatments: untreated control, warm water priming at 60°C for 15 minutes, 60% shallot extract for 30 minutes, and 70% coconut water for 30 minutes. Germination percentage was recorded every 6 hours (h) for 48 h, and physiological parameters, including T50, Mean Germination Time, Germination Index, Vigor Index, Relative Germination Index (RGI), and shoot emergence percentage, were calculated. Warm water reduced T50 to 18.48 h and shallot extract to 19.38 h, compared with 36.69 h in the control, indicating faster germination. These findings indicate that warm water and shallot extract are promising simple priming methods for improving the laboratory germination performance of commercial leafy vegetable seeds, although validation under nursery or field conditions is still needed.
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Abdul-Baki AA, Anderson JD. 1973. Vigor determination in soybean seed by multiple criteria. Crop Sci 13 (6): 630-633. https://doi.org/10.2135/cropsci1973.0011183X001300060013x.
Adhikari B, Olorunwa OJ, Horgan TE, Wilson J, Barickman TC, Li T, Bheemanahalli R. 2024. Seed priming attenuates the impact of salt stress and enhances lettuce yields. J Agric Food Res 15: 100947. https://doi.org/10.1016/j.jafr.2023.100947.
Bhatla SC, Kathpalia R. 2023. Seed dormancy and germination. In: Bhatla SC, Lal MA (eds.). Plant physiology, Development and Metabolism. Springer Nature Singapore, Singapore. https://doi.org/10.1007/978-981-99-5736-1_28.
Bhattarai K, Adhikari AP. 2023. Promoting urban farming for creating sustainable cities in Nepal. Urban Sci 7 (2): 54. https://doi.org/10.3390/urbansci7020054.
Bisi VSR, Silvestre W, Rodrigues LS. 2023. Germinative performance of lettuce (Lactuca sativa L.) landrace seeds for organic production. Rev Eletrônica Científica UERGS 9 (2): 74-82. https://doi.org/10.21674/2448-0479.92.74-82.
Cabrera-Santos D, Ordoñez-Salanueva CA, Sampayo-Maldonado S, Campos JE, Orozco-Segovia A, Flores-Ortiz CM. 2021. Chia (Salvia hispanica L.) seed soaking, germination, and fatty acid behavior at different temperatures. Agriculture 11 (6): 498. https://doi.org/10.3390/agriculture11060498.
Cano-Verdugo G, Flores-García BD, Núñez-Rocha GM, Ávila-Ortíz MN, Nakagoshi-Cepeda MAA. 2024. Impact of urban farming on health: A systematic review. J Public Health 46 (3): e500-e509. https://doi.org/10.1093/pubmed/fdae056.
Carganilla J, Aquino J, Dinay-og J, Cumahling GJ, Valdez M. 2025. Efficacy of coconut water as a seed priming agent for enhancing germination and emergence of rice. J Interdiscip Perspect 3 (11): 199-205. https://doi.org/10.69569/jip.2025.663.
Coolbear P, Francis A, Grierson D. 1984. The effect of low temperature pre-sowing treatment on the germination performance and membrane integrity of artificially aged tomato seeds. J Exp Bot 35 (11): 1609-1617. https://doi.org/10.1093/jxb/35.11.1609.
Ellis RH, Roberts EH. 1981. The quantification of ageing and survival in orthodox seeds. Seed Sci Technol 9: 373-409.
Ellouzi H, Ben Slimene Debez I, Amraoui S, Rabhi M, Hanana M, Alyami NM, Debez A, Abdelly C, Zorrig W. 2024. Effect of seed priming with auxin on ROS detoxification and carbohydrate metabolism and their relationship with germination and early seedling establishment in salt stressed maize. BMC Plant Biol 24: 704. https://doi.org/10.1186/s12870-024-05413-w.
Finch-Savage WE, Bassel GW. 2016. Seed vigour and crop establishment: Extending performance beyond adaptation. J Exp Bot 67 (3): 567-591. https://doi.org/10.1093/jxb/erv490.
Gebeyehu B. 2020. Review on the effect of seed storage period and storage environment on seed quality. Intl J Appl Agric Sci 6 (6): 185-190. https://doi.org/10.11648/j.ijaas.20200606.14.
Ghannad R, Haghjou MM, Raza A, Hasanuzzaman M. 2022. Induction of hydrolytic enzyme activities in dormant seeds of Dracocephalum kotschyi Boiss. causes improvement of germination and seedling vigor indices. Acta Physiol Plant 44 (4): 48. https://doi.org/10.1007/s11738-022-03381-y.
Han M, Kasim S, Yang Z, Deng X, Saidi NB, Uddin MK, Shuib EM. 2024. Plant extracts as biostimulant agents: A promising strategy for managing environmental stress in sustainable agriculture. Phyton-Intl J Exp Bot 93 (9): 2149-2166. https://doi.org/10.32604/phyton.2024.054009.
Hasanović M, Durmić-Pašić A, Karalija E. 2025. Seed priming beyond stress adaptation: Broadening the agronomic horizon. Agronomy 15 (8): 1829. https://doi.org/10.3390/agronomy15081829.
Kartika K, Lakitan B, Ria RP, Putri HH. 2021b. Effect of the cultivation systems and split fertilizer applications on the growth and yields of tatsoi (Brassica rapa subsp. narinosa). Trends Sci 18 (21): 344. https://doi.org/10.48048/tis.2021.344.
Kartika K, Lakitan B, Ria RP. 2021a. Hydro- and osmo-priming effects on upland rice exposed to drought conditions at vegetative and reproductive stages. CMUJ Nat Sci 20 (3): e2021053. https://doi.org/10.12982/CMUJNS.2021.053.
Khan MA, Ungar IA. 1998. Germination of the salt tolerant shrub Suaeda fruticosa from Pakistan: Salinity and temperature responses. Seed Sci Technol 26: 657-667.
Kumari KB, Pal A, Sen S, Ranjan RD, Atta K, Srivastava AK. 2026. Comparative efficacy of gibberellic acid and melatonin seed priming on germination and biochemical responses of maize under drought stress. Front Plant Sci 17: 1753761. https://doi.org/10.3389/fpls.2026.1753761.
Lakitan B, Fhadilah N, Muda SA, Irmawati, Ria RP. 2025. Impacts of artificial shading on growth and some morphological traits of romaine lettuce (Lactuca sativa L. cv. Romaine) cultivated in urban areas. Intl J Agric Technol 21 (4): 1329-1346. https://doi.org/10.63369/ijat.2025.21.4.1329-1346.
Liu L, Wang Z, Wu Y, Shen Y. 2025. Hormonal and storage metabolic regulation of germination in Toona sinensis. Horticulturae 11 (6): 685. https://doi.org/10.3390/horticulturae11060685.
Muda SA, Lakitan B, Ramadhani F, Juwinda. 2024. Butterhead lettuce growth under shallow water tables and its recovery on tropical urban ecosystem. Adv Hort Sci 38 (4): 327. https://doi.org/10.36253/ahsc-16233.
Oh S, Ahn E, Shi A, Mou B, Park S. 2025. Genome-wide association studies in lettuce reveal the interplay of seed age, color, and germination under high temperatures. Sci Rep 15: 733. https://doi.org/10.1038/s41598-024-84197-3.
Paparella S, Araújo SDS, Rossi G, Wijayasinghe M, Carbonera D, Balestrazzi A. 2015. Seed priming: State of the art and new perspectives. Plant Cell Rep 34 (8): 1281-1293. https://doi.org/10.1007/s00299-015-1784-y.
Reed RC, Bradford KJ, Khanday I. 2022. Seed germination and vigor: Ensuring crop sustainability in a changing climate. Heredity 128 (6): 450-459. https://doi.org/10.1038/s41437-022-00497-2.
Resigia E, Swasti E, Putri NE, Kusumawati A, Hasibuan SP. 2024. Effect of seed soaking with natural growth regulators on germination of red rice line SF 12-2-12. AGRIUM: Jurnal Ilmu Pertanian 27 (1): 44-53. https://doi.org/10.30596/agrium.v27i1.17636.
Rhaman MS. 2025. Seed priming before the sprout: Revisiting an established technique for stress-resilient germination. Seeds 4 (3): 29. https://doi.org/10.3390/seeds4030029.
Ria RP, Lakitan B, Sulaiman F, Kartika K, Suwignyo RA. 2020. Cross-ecosystem utilizing primed seeds of upland rice varieties for enriching crop diversity at riparian wetland during dry season. Biodiversitas 21 (7): 3008-3017. https://doi.org/10.13057/biodiv/d210718.
Ria RP, Lakitan B, Sulaiman F, Yakup Y. 2023. Searching for suitable cultivation system of Swiss chard (Beta vulgaris subsp. cicla (L.) W.D.J. Koch) in the tropical lowland. J Hortic Res 31 (1): 81-90. https://doi.org/10.2478/johr-2023-0022.
Ruksiriwanich W, Khantham C, Muangsanguan A, Chittasupho C, Rachtanapun P, Jantanasakulwong K, Barba FJ. 2022. Phytochemical constitution, anti-inflammation, anti-androgen, and hair growth-promoting potential of shallot (Allium ascalonicum L.) extract. Plants 11 (11): 1499. https://doi.org/10.3390/plants11111499.
Sharaf Z, Habibi N, Fakhri A, Faqiri SM. 2026. Seed priming to induce seed germination, seedling growth and enzymatic activity in watermelon. Sci Nat 113: 43. https://doi.org/10.1007/s00114-026-02100-8.
Talpur A, Khaskheli AA, Talpur MYMK, Nizamani N, Hassan G. 2024. Optimizing potential of coconut water as an organic priming agent for jujube (Ziziphus mauritiana L.). J Hortic Agric Sci 1 (1): 53-60. https://doi.org/10.63459/jhas.2024.1.10.
Taylor AG. 2020. Seed Storage, Germination, Quality, and Enhancements. The Physiology of Vegetable Crops. CABI, Wallingford, UK. https://doi.org/10.1079/9781786393777.0001.
Thirusendura Selvi D, Saraswathy S. 2018. Seed viability, seed deterioration and seed quality improvements in stored onion seeds: A review. J Hortic Sci Biotechnol 93 (1): 1-7. https://doi.org/10.1080/14620316.2017.1343103.
Upretee P, Bandara MS, Tanino KK. 2024. The role of seed characteristics on water uptake preceding germination. Seeds 3 (4): 559-574. https://doi.org/10.3390/seeds3040038.
Wei J, Zhang Q, Zhang Y, Yang L, Zeng Z, Zhou Y, Chen B. 2024. Advance in the thermoinhibition of lettuce (Lactuca sativa L.) seed germination. Plants 13 (15): 2051. https://doi.org/10.3390/plants13152051.
Yunindanova MB, Budiastuti MS, Purnomo D. 2018. The analysis of endogenous auxin of shallot and its effect on the germination and the growth of organically cultivated melon (Cucumis melo). IOP Conf Ser Earth Environ Sci 215 (1): 012018. https://doi.org/10.1088/1755-1315/215/1/012018.
Zulfiqar F. 2021. Effect of seed priming on horticultural crops. Sci Hortic 286: 110197. https://doi.org/10.1016/j.scienta.2021.110197.