Plant-based elicitor improves nutrient efficiency and sustains rice yield under reduced fertilizer in a one-season field study
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Abstract. Harwanto, Purwanti EW, Sunaryono JG, Wandansari NR. 2026. Plant-based elicitor improves nutrient efficiency and sustains rice yield under reduced fertilizer in a one-season field study. Asian J Agric 10 (1): g100179. https://doi.org/10.13057/asianjagric/g100179. Excessive dependence on chemical fertilizers raises environmental concerns and reduces long-term soil fertility. A plant-based elicitor containing bioactive compounds (flavonoids, phenols, tannins) and phytohormones (IAA, cytokinins, gibberellins) such as Biosaka, has been promising elicitors to enhance nutrient efficiency and crop resilience. A field experiment was conducted in East Java, Indonesia, using a randomized block design with five treatments: 100% inorganic fertilizer (control), 100% inorganic fertilizer + Biosaka, 75% inorganic fertilizer + Biosaka, 50% inorganic fertilizer + Biosaka, and 25% inorganic fertilizer + Biosaka. Each treatment was replicated four times. Variables measured included plant growth, yield components, straw nutrient content (N, P, K), and soil chemical properties (pH, organic C, available N, P, K). The combination of 50% inorganic fertilizer + Biosaka maintained straw nitrogen comparable to full fertilizer and resulted in higher straw phosphorus content. The combination of 100% inorganic fertilizer + Biosaka produced the highest straw Kalium (0.53%) and milled rice weight (454.41 g 2 m-2). Biosaka treatments were associated with higher soil pH (6.30), organic carbon (0.99%), and available N and K compared to non-Biosaka treatments. Biosaka supplements have not compensated for the nutrient deficiency in the treatments with excessive fertilizer reduction (≤25%). It resulted in lower nutrient content and reduced milled rice weight (265.09 g 2 m-2). This study provides novel evidence that integrating Biosaka treatment with 50% inorganic fertilizer can sustain rice yield equivalently to the conventional 100% inorganic fertilizer application.
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Anas M, Liao F, Verma KK, Sarwar MA, Mahmood A, Chen ZL, Li Q, Zeng XP, Liu Y, Li YR. 2020. Fate of nitrogen in agriculture and environment: Agronomic, eco-physiological and molecular approaches to improve nitrogen use efficiency. Biol Res 53: 47. https://doi.org/10.1186/s40659-020-00312-4.
Ansar M, Manurung R, Barki H, Suwandi, Pambudy R, Fahmid IM, Sugihartono U. 2023. Elisitor Nuswantara: Biosaka (1st ed.). IPB Press, Bogor. [Indonesian]
Badan Pusat Statistik (BPS). 2023. Paddy Harvested Area and Production in Indonesia 2023. BPS-Statistics Indonesia, Jakarta. https://www.bps.go.id/en/pressrelease/2023/10/16/2037/paddy-harvested-area-and-production-in-indonesia-2023--preliminary-figures-.html.
Balittanah. 2009. Petunjuk Teknis Analisis Kimia Tanah, Tanaman, Air, dan Pupuk Edisi 2. Balai Penelitian Tanah, Badan Penelitian dan Pengembangan Pertanian, Bogor. [Indonesian]
Dai Z, Liu G, Chen H, Chen C, Wang J, Ai S, Wei D, Li D, Ma B, Tang C, Brookes PC, Xu J. 2020. Long-term nutrient inputs shift soil microbial functional profiles of phosphorus cycling in diverse agroecosystems. ISME J 14: 757-770. https://doi.org/10.1038/s41396-019-0567-9.
Dias MC, Pinto DCGA, Silva AMS. 2021. Plant flavonoids: Chemical characteristics and biological activity. Molecules 26 (17): 5377. https://doi.org/10.3390/molecules26175377.
du Jardin P. 2015. Plant biostimulants: Definition, concept, main categories and regulation. Sci Hortic 196: 3-14. https://doi.org/10.1016/j.scienta.2015.09.021.
Gao X, Zhang Y, He Z, Fu X. 2017. Gibberellins. In: Li J, Li C, Smith SM (eds.). Hormone Metabolism and Signaling in Plants. Elsevier, Amsterdam. https://doi.org/10.1016/B978-0-12-811562-6.00004-9.
Govindarajan RK, Revathi S, Rameshkumar N, Krishnan M, Kayalvizhi N. 2016. Microbial tannase: Current perspectives and biotechnological advances. Biocatal Agric Biotechnol 6: 168-175. https://doi.org/10.1016/j.bcab.2016.03.011.
Guo Z, Ye W, Wang H, He W, Tia Y, Hu G, Lou Y, Pan H, Yang Q, Zhuge Y. 2024. Straw and phosphorus applications promote maize (Zea mays L.) growth in saline soil through changing soil carbon and phosphorus fractions. Front Microbiol 15: 1336300. https://doi.org/10.3389/fpls.2024.1336300.
Hasanuzzaman M, Bhuyan MHMB, Nahar K, Hossain MS, Al Mahmud J, Hossen MS, Masud AAC, Moumita, Fujita M. 2018. Potassium: A vital regulator of plant responses and tolerance to abiotic stresses. Agronomy 8 (3): 31. https://doi.org/10.3390/agronomy8030031.
Kader MA. 2021. Soil Sampling Guidelines: Strengthening Regional Collaboration on Soil Analysis. Suva: The Pacific Community (SPC) dan Commonwealth Scientific and Industrial Research Organisation (CSIRO). University of South Pacific Samoa Campus, Samoa. https://research.csiro.au/pacsoils/wp-content/uploads/sites/404/2021/12/2-Soil-sampling-manual.pdf.
Kumar SR, David EM, Pavithra GJ, Kumar GS, Lesharadevi K, Akshaya S, Basavaraddi C, Navyashree G, Arpitha PS, Sreedevi P, Zainuddin K, Firdous S, Babu BR, Prashanth MU, Ravikumar G, Basavaraj P, Chavana SK, Kumar VMLD, Parthasarathi T, Subbian E. 2024. Methane-derived microbial biostimulant reduces greenhouse gas emissions and improves rice yield. Front Plant Sci 15: 1432460. https://doi.org/10.3389/fpls.2024.1432460.
Liao Z, Yu H, Duan J, Yuan K, Yu C, Meng X, Kou L, Chen M, Jing Y, Liu G, Smith SM, Li J, 2019. SLR1 inhibits MOC1 degradation to coordinate tiller number and plant height in rice. Nat Commun 10: 2738. https://doi.org/10.1038/s41467-019-10667-2.
Liu J, Yin X, Kou C, Thimmappa R, Hua X, Xue Z. 2024. Classification, biosynthesis, and biological functions of triterpene esters in plants. Plant Commun 5 (4): 100845. https://doi.org/10.1016/j.xplc.2024.100845.
Liu Y, Ding W, He P, Xu X, Zhou W. 2024. Estimating thresholds of nitrogen, phosphorus and potassium fertilizer rates for rice cropping systems in China. Front Plant Sci 15: 1470774. https://doi.org/10.3389/fpls.2024.1470774.
Meena VS, Meena SK, Verma JP, Kumar A, Aeron A, Mishra PK, Bisht JK, Pattanayak A, Naveed M, Dotaniya ML. 2017. Plant Beneficial Rhizospheric Microorganism (PBRM) strategies to improve nutrients use efficiency: A review. Ecol Eng 107: 8-32. https://doi.org/10.1016/j.ecoleng.2017.06.058.
Naik PM, Al-Khayri JM. 2016. Abiotic and biotic elicitors: Role in secondary metabolites production through in vitro culture of medicinal plants. In: Shanker AK, Shanker C (eds.). Abiotic and Biotic Stress in Plants: Recent Advances and Future Perspectives. InTechOpen, London. https://doi.org/10.5772/61442.
Pang F, Li Q, Solanki MK, Wang Z, Xing YX, Dong DF. 2024. Soil phosphorus transformation and plant uptake driven by phosphate-solubilizing microorganisms. Front Microbiol 15: 1383813. https://doi.org/10.3389/fmicb.2024.1383813.
Ramli A, Adrianto B, Rachmat R. 2024. Application of Biosaka and NPK organic fertilizers to increase the growth of rice plants (Oryza sativa L.). Jurnal Agrisistem 20 (1): 24-30. https://doi.org/10.52625/j-agr.v20i1.318. [Indonesian]
Roopa S, Mishra T, Bhattacharya S, Bhadra A, Singh SR, Shrivastava R, Patil SJ. 2023. Role of IAA in plant growth, development, and interaction with other phytohormones. Eur Chem Bull 12 (5): 5293-5297. https://doi.org/10.48047/ecb/2023.12.si5a.0448.
Rouphael Y, Colla G. 2020. Editorial: Biostimulants in agriculture. Front Plant Sci 11: 40. https://doi.org/10.3389/fpls.2020.00040.
Saryanto, Kirono R, Najib KH, Ilman AA, Jaria A, Rahmaningtias D, Madiasta, Don ME, Amelia NN, Winarno H, Madyo L. 2024. Pelatihan pembuatan pupuk Biosaka, upaya perlindungan tanaman berbasis ekologi untuk menjaga kelestarian lingkungan. Solusi Bersama Jurnal Pengabdian dan Kesejahteraan Masyarakat 1 (3): 72-78. https://doi.org/10.62951/solusibersama.v1i3.376.
Sulaeman Y, Aryati V, Suprihatin A et al. 2024. Yield gap variation in rice cultivation in Indonesia. Open Agric 9 (1): 20220241. https://doi.org/10.1515/opag-2022-0241.
Thepbandit W, Papathoti NK, Daddam JR, Hoang NH, Le Thanh T, Saengchan C, Buensanteai K. 2023. In vitro and in silico studies of salicylic acid on systemic induced resistance against bacterial leaf blight disease and enhancement of crop yield. J Integr Agric 22 (1): 170-184. https://doi.org/10.1016/j.jia.2022.08.112.
Touch V, Tan DKY, Cook BR, Liu DL, Cross R, Anh T, Utomo A, Yous S, Grunbuhel C, Cowie A. 2024. Smallholder farmers’ challenges and opportunities: Implications for agricultural production, environment and food security. J Environ Manag 370: 122536. https://doi.org/10.1016/j.jenvman.2024.122536.
Wang J, Wang R, Liu L, Zhang W, Yin Z, Guo R, Wang D. 2025. Integrated physiological, transcriptomic and metabolomic analyses of the response of rice to aniline toxicity. Intl J Mol Sci 26 (2): 582. https://doi.org/10.3390/ijms26020582.
Wei C, Cao B, Gao S, Liang H. 2025. Co-incorporation of green manure and rice straw increases rice yield and nutrient utilization. Plants 14 (11): 1678. https://doi.org/10.3390/plants14111678.
Yuan S, Stuart AM, Laborte AG, Edreira JIR, Dobermann A, Kien LVN, Thúy LT, Paothong K, Traesang P, Tint KM, San SS, II MQV, Quicho ED, Pame ARP, Then R, Flor RJ, Thon N, Agus F, Agustiani N, Deng N, Li T, Grassini P. 2022. Southeast Asia must narrow down the yield gap to continue to be a major rice bowl. Nat Food 3: 217-226. https://doi.org/10.1038/s43016-022-00477-z.
Zhou G, Wang M, Zhu H, Wang J, Zhang S. 2025. Effects of organic carbon, inorganic phosphorus, and phosphorus-solubilizing bacteria on maize growth, nutrient uptake, and rhizosphere phosphorus availability. Front Plant Sci 16: 1644448. https://doi.org/10.3389/fpls.2025.1644448.
Zhou W, Yan L, Fu Z, Guo H, Zhang W, Liu W, Ye Y, Long P. 2023. Increasing planting density and reducing N application improves yield and grain filling at two sowing dates in double-cropping rice systems. Plants 12 (12): 2298. https://doi.org/10.3390/plants12122298.
Zhou Z, Zhang S, Jiang N, Xiu W, Zhao J, Yang D. 2022. Effects of organic fertilizer incorporation practices on crops yield, soil quality, and soil fauna feeding activity in the wheat-maize rotation system. Front Environ Sci 10: 1058071. https://doi.org/10.3389/fenvs.2022.1058071.