Abstract. Shanmugam S, Jeyaprabakaran G, Rajendran K. 2020. Medicinal trees from home gardens of urban areas in Madurai District of Tamil Nadu, Southern India. Asian J Ethnobiol 3: 10-15. Home gardens provide a diverse and stable supply of socio-economic products. They serve as conservation spots of many useful and rare medicinal plants. Present research work aims to elucidate the medicinally important trees growing in home gardens and their role in the health care of the inhabitants living in four urban areas of Madurai District, Tamil Nadu, India. Data was collected by interviewing 20 informants (14 male and 6 female) regarding the ethnomedicinal uses of plants prescribed for the treatment of several ailments in total. We recorded a total of 63 medicinal remedies prepared from 54 tree species belonging to 52 genera and 27 families used to treat 35 illness. It was found that the most dominant family was Apocynaceae (5 species). Leaf was the mostly used plant part (28.56%) and using raw material (20.64%) was the most was treating a particular disease. Phytochemical and pharmacological values of these medicinally important trees should be tested and awareness programs should also be conducted to encourage the people to grow many more useful plants in their home gardens.