Abstract. Pitasari IS, Setyono P, Wiryanto. 2022. Analysis of water quality and heavy metal content of chromium in water, sediment, and flesh of tilapia (Oreochromis niloticus) in the Premulung River, Surakarta, Central Java, Indonesia. Intl J Bonorowo Wetlands 12: 56-62. The river is a lotic ecosystem that plays an essential role in living things' lives, including as a habitat for aquatic organisms, drainage channels, and water sources for human needs. The Premulung River crosses the city of Surakarta, Central Java, Indonesia. The area along the Premulung River is surrounded by settlements, markets, textile industries, batik, screen printing, and hospitals, which produce waste that has the potential to pollute and degrade water quality. This study is aimed to determine the water quality and content of heavy chromium metal (Cr) in water, sediment, and tilapia meat (Oreochromis niloticus Linnaeus, 1758) in the Premulung River, Surakarta City, as well as the relationship between chromium content in water, in sediment and fish meat. This research was conducted by field observation at three stations of the Premulung River flows, namely under the Kleco Bridge, Griyan, and Jongke. The parameter data of temperature, TDS, pH, DO, BOD, COD, and Cr water compared to PPRI No. 82 of 2001 (class 2 water); chromium sediment compared to ANZECC Year 2000; Fish meat chromium compared to CFSA 2012. The Premulung River water quality was analyzed using the STORET method, while the Pearson correlation analyzed the relationship between chromium in water, sediment, and fish meat. The results showed that the water quality of the Premulung River in Surakarta City was moderately polluted. The heavy metal content of Premulung River chromium water (ttd to 0.0344) mg/L and chromium sediment (1.307-4.948) mg/kg still meets the standard quality, while the tilapia meat chromium (1.958-3.535) mg/kg exceeds the standard quality. The relationship between chromium water content was very strong and directly proportional (r= 0.997) with chromium fish meat, while chromium sediment with chromium fish meat was inversely proportional (r= -0.470).