Effect of giving nanochitosan preparations ethanol extract of neem leaves (Azadirachta indica) against pancreatic histology of white rat male (Rattus norvegicus) Sprague Dawley

##plugins.themes.bootstrap3.article.sidebar##

PDF
DOI https://doi.org/10.13057/cellbioldev/v060103
Issue
Vol. 6 No. 1 (2022)
Section
Articles

##plugins.themes.bootstrap3.article.main##

SUCI HANDAYANI ♥
Department of Biology, Faculty of Science and Mathematics, Universitas Diponegoro. Jl. Prof. H. Soedarto, Tembalang, Semarang 50275, Central Java, Indonesia
AGUNG JANIKA SITASIWI
Department of Biology, Faculty of Science and Mathematics, Universitas Diponegoro. Jl. Prof. H. Soedarto, Tembalang, Semarang 50275, Central Java, Indonesia
SRI ISDADIYANTO
Department of Biology, Faculty of Science and Mathematics, Universitas Diponegoro. Jl. Prof. H. Soedarto, Tembalang, Semarang 50275, Central Java, Indonesia
SITI MUFLICATUN MARDIATI
Department of Biology, Faculty of Science and Mathematics, Universitas Diponegoro. Jl. Prof. H. Soedarto, Tembalang, Semarang 50275, Central Java, Indonesia

Abstract

Abstract. Handayani S, Sitasiwi AJ, Isdadiyanto S, Mardiati SM. 2022. Effect of giving nanochitosan preparations ethanol extract of neem leaves (Azadirachta indica) against pancreatic histology of white rat male (Rattus norvegicus) Sprague Dawley. Cell Biol Dev 6: 13-19. The selection of herbal plants as a treatment in the community is considered safer, more practical, and cheaper than synthetic drugs. Neem (Azadirachta indica A.Juss.) is one of the herbal plants that have the potential as an antioxidant. However, the low bioavailability of drugs and the distribution of active compounds in herbal plants are constraints in administering drugs orally. The solution to overcome this problem is to prepare the test material in the form of nanochitosan. This study aimed to analyze the effect of nanochitosan ethanol extract of neem (A. indica) leaf extract on the pancreas histology of male Sprague Dawley rats. This study used a completely randomized design (CRD) with 32 rats aged 2 months divided into 4 treatments, and each treatment consisted of 6 replications. P0 (normal rat group treated with 2 mL distilled water, P1 (normal rats induced with Natrium tripolifosfat "NaTPP" and 2 mL chitosan), P2 (normal rat group induced by nanochitosan neem leaf ethanol extract 1:0.5), P3 (normal rat group induced nanochitosan ethanol extract of neem leaves 1:1). Data were analyzed by ANOVA test with a significance level of 5%. Data that were not normally distributed were tested by the Kruskal Wallis test and Duncan's test. The results showed that the administration of nanochitosan ethanol extract of neem leaves 1:0.5 and 1:1 had no significant effect on the diameter parameter of the islets of Langerhans (P?0.05) but had a significant effect on the parameters of pancreatic weight and damage scoring of the islets of Langerhans (P?0.05). The administration of nanochitosan preparations of ethanolic extract of neem leaves can deliver bioactive compounds neem to the pancreas and minimize damage to the cells that make up the islets of Langerhans due to toxic neem compounds.

2017-01-01

##plugins.themes.bootstrap3.article.details##

References
Abror YK, Woelansari ED, Suhariyadi S. 2018. Immunomodulator of ethanol extracts of the leaves Azadirachta indica against macrophage peritoneal cell in mice induced the vaccine BCG. J Teknologi Laboratorium 8 (1): 8-14. DOI: 10.29238/teknolabjournal.v7i1.110.
Ajazuddin, Saraf S. 2010. Applications of novel drug delivery system for herbal formulations. J Fitoterapia 81 (7): 680-689. DOI: 10.1016/j.fitote.2010.05.001.
Ambarwati. 2011. Neem as antibacterial, antifungal and biopesticide. J Health 4 (2): 154-163.
Berata IK, Samsuri I. 2017. Histopathology of the small intestines of male white rats given dexamethasone and vitamin E. Buletin Veteriner Udayana 9 (1): 47-53.
Darusman HS, Nugroho SW, Munggaran FA, Sajuthi D. 2018. Teknik penanganan kendali hewan sesuai kaidah kesejahteraan hewan meningkatkan akurasi pengukuran profil hemodinamika tikus laboratorium. J Veteriner 19 (2): 208-214. DOI: 10.19087/jveteriner.2018.19.2.208. [Indonesian]
Fathoni M, Yanuwiadi B, Leksono AS. 2013. The effectiveness of combination mahogany (Swietenia mahogany) seed and Sour Sup (Annona muricata) leaf pesticide to the time of stop feeding and LC50 mortality on armyworm (Spodoptera litura F.). J Biodivers Environ Sci 3 (11): 71-77.
Hasna ASN, Isdadiyanto S, Sitasiwi AJ. 2022. Histopathology of rats intestinal treated with high-fat diet and neem leaf extract. J Pro-Life 9 (1): 393-407. DOI: 10.33541/jpvol6Iss2pp102.
Juanda J, Jayadi EM. 2015. Effect of neem leaf extract (Azadirachta indica A. Juss) on mortality of pests of chili fruit flies (Bactrocera dorsalis L.). J Biota 8 (1): 97-106. DOI: 10.20414/jb.v8i1.62.
Kafshgari MH, Mohammad K, Mobina K, Sahar K. 2011. Reinforcement of chitosan nanoparticles obtained by an ionic crosslinking process. Iran Polym J 20 (5): 445-456.
Kakkar V, Singh S, Singla D, Kaur P. 2011. Exploring solid lipid nanoparticles to enhance the oral bioavailability of curcumin. Mol Nutr Food Res 55: 495-503. DOI: 10.1002/mnfr.201000310.
Martien R, Adhyatmika A, Irianto ID, Farida V, Sari DP. 2012. The development of nanoparticle technology as a drug delivery system. Pharm Mag 8 (1): 133-144.
Mescher A. L. 2016. Junqueira's Basic Histology Text and Atlas. 14th Edition. McGraw-Hill, USA
Nugroho A, Adianto C, Patria Y. 2020. Nano-Androcerum: Inovasi wound healinggel dari nanopartikel daun binahong dan kayu manis pada luka kronis. Berkala Ilmiah Mahasiswa Farmasi Indonesia 7 (1): 026-042. DOI: 10.48177/bimfi.v7i1.11.
Prameswari OM, Widjanarko SB. 2014. Test of the effects of wangi pandan leaf water extract on reducing blood glucose levels and histopathology of diabetes mellitus rats. J Food Agroind 2 (2): 16-27.
Prasetiowati AL, Prasetya AT, Wardani S. 2018. Synthesis of silver nanoparticles with bioreductors of starfruit leaf extract (Averrhoa bilimbi L.) testing its activity as antibacterial. Indones J Chem Sci 7 (2): 160.
Pristiani D, Astuti P. 2005. Isolation of antibacterial compounds from chloroform extract of neem (Azadirachta indica A. Juss.) leaves guided by bioautography. Biofarmasi 3 (2): 43-46. DOI: 10.13057/biofar/f030202.
Rawat M, Singh D, Saraf S. 2006. Nanocarriers: Promising vehicle for bioactive drugs. Biol Pharm Bull 29 (9): 1790-1798. DOI: 10.1248/bpb.29.1790.
Septiana DI, Estiningdriati I, Ismadi U. 2012. Effect of using rations ripened with papaya leaf extract (Carica Papaya L) on blood protein and hemoglobin in Rattus norvegicus. Anim Agric J 1 (1): 461-470. DOI: 10.22219/sm.Vol17.SMUMM2.18803.
Setiadi E, Peniati E, Susanti RSR. 2020. Effect of aloe bark extract on blood sugar levels and histopathological picture of alloxan induced rat pancreas. J Life Sci 9 (2): 171-185. DOI: 10.15294/biosaintifika.v11i3.20896.
Sitasiwi AJ, Isdadiyanto S, Mardiati SM. 2019. Tracking protein expression in the testes of mice (Mus musculus) after exposure to ethanol extract of neem (Azadirachta indica) leaves. J Vet Sci 37 (1): 90-96. DOI: 10.15294/biosaintifika.v14il.31470.
Sitasiwi AJ, Jannah SN, Isdadiyanto S, Annisa T, Hermawati CM, Sari AM, Putra MAD. 2021. Study of the pineapple peel vinegar potency in restoring the gonadosomatic index of the diabetic rats. J Phys: Conf Ser 1943 (2021): 012067. DOI: 10.1088/1742-6596/1943/1/012067.
Soraya M. 2021. Effect of Kalangkala (Litsea angulata) Seed Extract as Antimicrobial on the Growth of Staphylococcus aureus Bacteria. [Thesis]. Universitas Muhammadiyah Malang, Malang. [Indonesian]
Suhendro, Susraini AAAN, Saputra H, Sriwidyani NP. 2018. Oral administration of neem (Azaradiracta indica Juss) leaf extract increase cyclin D1 expression in hepatocyte regeneration in acetaminophen-induced hepatotoxic wistar rats. Bali Med J 7 (1): 12-16. DOI: 10.15562/bmj.v7i1.700.
Tandi J, Rizky M, Mariani R, Alan F. 2017. Test of effects of ethanol extract of breadfruit leaves (Artocarpus altilis) on reduction of blood glucose levels, total cholesterol and pancreatic histopathological picture of male white rat (Rattus norvegicus). Hyperchol Diabet 1 (8): 384-396. DOI: 10.52434/jfb.v13i1.1114.
Treuting PM, Dintzis SM, Montine KS. 2018. Comparative Anatomy and Histology: A Mouse, Rat, and Human Atlas. Elsevier/Academic Press, London, United Kingdom.
Walean M, Melpin R, Rondonuwu M, Pinontoan KF. 2020. Histopathology improvement of hyperglycemic rat pancreas after administration of pakoba bark bark ethanol extract. Biosph Sci Mag: Sci J 37 (1): 43-48. DOI: 10.4314/njps.v24i2.52927.
Wowiling J. 2013. Vegetable Pesticide Neem (Azadirachta indica A. Juss) in Controlling Plant Pest Organisms (OPT). Sinar Tani, Jakarta. [Indonesian]