Protective effect of vitamin C against alcohol induced lungs toxicity in adult male Wistar rats
##plugins.themes.bootstrap3.article.sidebar##
Issue
Section
Articles
##plugins.themes.bootstrap3.article.main##
Abstract
Abstract. Oyesola OA, Osonuga IO, George ET. 2023. Protective effect of vitamin C against alcohol induced lungs toxicity in adult male Wistar rats. Cell Biol Dev 7: 35-40. The lungs are the major organs in the respiratory system. The toxic effect of alcohol on the lungs leads to cell death and loss of function. This study aimed to investigate the protective effects of vitamin C on alcohol-induced lung toxicity in male Wistar rats. Forty male Wistar rats were acclimated for 14 days and randomly divided into eight groups. Group A was the control and received only distilled water. Group B was given alcohol, while groups C, D, and E received varying doses of vitamin C. Groups F, G, and H received alcohol, followed by vitamin C. After 21 days of treatment, the rats' lungs were collected and evaluated for various parameters, including antioxidant enzyme activity (Catalase (CAT), Glutathione (GSH), and Superoxide Dismutase (SOD), lipid peroxidation levels (malodialdehyde (MDA), carbon dioxide (CO2) levels in the blood, and histopathological changes. The results indicated that rats that received only alcohol had increased lipid peroxidation (MDA) levels, reduced antioxidant enzyme activity (CAT, SOD, and GSH) in the lungs, high CO2 levels in the blood, dilation of the alveolar sac, and disorientation of the bronchioles. However, groups treated with alcohol and vitamin C exhibited increased antioxidant enzyme activity, reduced lipid peroxidation and CO2 content of the blood, regenerative changes, and improvement in the histo-architecture of the lungs. Vitamin C has demonstrated protective properties against alcohol-induced lung toxicity.
2017-01-01
##plugins.themes.bootstrap3.article.details##
References
Adewoyin M, Ibrahim M, Roszaman R, Md Isa, ML, Mat Alewi NA, Abdul Rafa AA, Anuar MNN. 2017. Male infertility: The effect of natural antioxidants and phytocompounds on seminal oxidative stress. Diseases 5 (1): 9. DOI: 10.3390/diseases5010009.
Ares AM, Nozal MJ, Bernal J. 2013. Extraction, chemical characterization and biological activity determination of broccoli health promoting compounds. J Chromatogr A 1313: 78-95. DOI: 10.1016/j.chroma.2013.07.051.
Bhattacharya S. 2015. Reactive oxygen species and cellular defense system. In: Rani V, Yadav U (eds). Free Radicals in Human Health and Disease. Springer, New Delhi. DOI: 10.1007/978-81-322-2035-0_2.
Brown SD, Brown LAS. 2012. Ethanol (E t OH)?induced TGF??1 and reactive oxygen species production are necessary for E t OH?induced alveolar macrophage dysfunction and induction of alternative activation. Alcohol Clin Exp Res 36 (11): 1952-1962. DOI: 10.1111/j.1530-0277.2012.01825.x.
Buege JA, Aust SD. 1978. [30] Microsomal lipid peroxidation. Methods Enzymol 52: 302-310. DOI: 10.1016/s0076-6879(78)52032-6.
Capozzi V, Russo P, Dueñas MT, López P, Spano G. 2012. Lactic acid bacteria producing B-group vitamins: A great potential for functional cereals products. Appl Microbiol and Biotechnol 96: 1383-1394. DOI: 10.1007/s00253-012-4440-2.
Carr AC, Maggini S. 2017. Vitamin C and immune function. Nutrients 9 (11): 1211. DOI: 10.3390/nu9111211.
Dasí F, Amor M, Sanz F, Codoñer-Franch P, Navarro-García MM, Escribano A. 2013. Oxidative stress in serum of patients with alpha-1 antitrypsin deficiency. Eur Respir J 42: 57.
DeLeve LD, Wang X, Kuhlenkamp JF, Kaplowitz N. 1996. Toxicity of azathioprine and monocrotaline in murine sinusoidal endothelial cells and hepatocytes: The role of glutathione and relevance to hepatic venoocclusive disease. Hepatology 23 (3): 589-599. DOI: 10.1002/hep.510230326.
Di Rosanna P, Salvatore C. 2012. Reactive oxygen species, inflammation, and lung diseases. Curr Pharm Design 18 (26): 3889-3900. DOI: 10.2174/138161212802083716.
Domej W, Oettl K, Renner W. 2014. Oxidative stress and free radicals in COPD–implications and relevance for treatment. Intl J Chron Obstr Pulm Dis 9: 1207-1224. DOI: 10.2147/COPD.S51226.
Downs CA, Trac D, Brewer EM, Brown LA, Helms MN. 2013. Chronic alcohol ingestion changes the landscape of the alveolar epithelium. BioMed Res Intl 2013: 470217. DOI: 10.1155/2013/470217.
Grievink L, Smit HA, Ocké MC, van‘t Veer P, Kromhout D. 1998. Dietary intake of antioxidant (pro)-vitamins, respiratory symptoms and pulmonary function: The MORGEN study. Thorax 53 (3): 166-171. DOI: 10.1136/thx.53.3.166.
Hartmann SE, Kissel CK, Szabo L, Walker BL, Leigh R, Anderson TJ, Poulin MJ. 2015. Increased ventilatory response to carbon dioxide in COPD patients following vitamin C administration. ERJ Open Res 1 (1): 00017-2015. DOI: 10.1183/23120541.00017-2015.
Hoth KF, Ford DW, Sandhaus RA, Strange C, Wamboldt FS, Holm KE. 2012. Alcohol use predicts ER visits in individuals with Alpha-1 Antitrypsin Deficiency (AATD) associated COPD. Intl J Chron Obstr Pulm Dis 9 (4): 417-425. DOI: 10.3390/jcm11133594.
Janciauskiene S. 2020. The beneficial effects of antioxidants in health and diseases. Chronic Obstr Pulm Dis 7 (3): 182. DOI: 10.15326/jcopdf.7.3.2019.0152.
Jing L, Jin CM, Li SS, Zhang FM, Yuan L, Li WM, Sang Y, Li S, Zhou LJ. 2012. Chronic alcohol intake-induced oxidative stress and apoptosis: role of CYP2E1 and calpain-1 in alcoholic cardiomyopathy. Mol Cell Biochem 359: 283-292. DOI: 10.1007/s11010-011-1022-z.
Kahraman A, Çakar H, Köken T. 2012. The protective effect of quercetin on long-term alcohol consumption-induced oxidative stress. Molecular Biol Rep 39: 2789-2794. DOI: 10.1007/s11033-011-1037-2.
Kaphalia L, Calhoun WJ. 2013. Alcoholic lung injury: Metabolic, biochemical and immunological aspects. Toxicol Lett 222 (2): 171-179. DOI: 10.1016/j.toxlet.2013.07.016.
Liu X, Chen Z. 2017. The pathophysiological role of mitochondrial oxidative stress in lung diseases. J Transl Med 15 (1): 1-13. DOI: 10.1186/s12967-017-1306-5.
Liu Z, Ren Z, Zhang J, Chuang CC, Kandaswamy E, Zhou T, Zuo L. 2018. Role of ROS and nutritional antioxidants in human diseases. Front Physiol 9: 477. DOI: 10.3389/fphys.2018.00477.
Lodovici M, Bigagli E. 2011. Oxidative stress and air pollution exposure. J Toxicol 2011: 487074. DOI: 10.1155/2011/487074.
Lykkesfeldt J, Tveden-Nyborg P. 2019. The pharmacokinetics of vitamin C. Nutrients 11 (10): 2412. DOI: 10.3390/nu11102412.
Macdonald IO, Olusola OJ, Osaigbovo UA. 2010. Effects of chronic ethanol administration on body weight, reduced Glutathione (GSH), Malondialdehyde (MDA) levels and glutathione-s-transferase activity (GST) in rats. NY Sci J 3 (4): 3947.
Melamed P, Melamed F. 2014. Chronic metabolic acidosis destroys pancreas. J Pancreas 15 (6): 552-560. DOI: 10.6092/1590-8577/2854.
Molnar C, Gair J. 2013. 11.3 Circulatory and Respiratory Systems. Concepts of Biol-1st Canadian Edition. BCcampus. Retrieved from https://opentextbc.ca/biology/
National Research Council. 2011. Guide for the Care and Use of Laboratory Animals. The National Academies Press, Washington D.C.
Neuman MG, French SW, French BA, Seitz HK, Cohen LB, Mueller S, Osna NA, Kharbanda KK, Seth D, Bautista A, Thompson KJ. 2014. Alcoholic and non-alcoholic steatohepatitis. Exp Mol Pathol 97 (3): 492-510. DOI: 10.1016/j.yexmp.2014.09.005.
Rehm J, Gmel Sr GE, Gmel G, Hasan OS, Imtiaz S, Popova S, Probst C, Roerecke M, Room R, Samokhvalov AV, Shield KD. 2017. The relationship between different dimensions of alcohol use and the burden of disease—an update. Addiction 112 (6): 968-1001. DOI: 10.1111/add.13757.
Rungratanawanich W, Qu Y, Wang X, Essa MM, Song BJ. 2021. Advanced Glycation End Products (AGEs) and other adducts in aging-related diseases and alcohol-mediated tissue injury. Exp Mol Med 53 (2): 168-188. DOI: 10.1038/s12276-021-00561-7.
Sabino JPJ, Silva ALD, Resstel LB, Antunes-Rodrigues J, Glass ML, Branco LG. 2014. Effect of chronic ethanol exposure on rat ventilatory responses to hypoxia and hypercapnia. Clinics 69: 360-366. DOI: 10.6061/clinics/2014(05)11.
Schloss J, Lauche R, Harnett J, Hannan N, Brown D, Greenfield T, Steel A. 2020. Efficacy and safety of vitamin C in the management of acute respiratory infection and disease: A rapid review. Adv Integr Med 7(4): 187-191. DOI: 10.1016/j.aimed.2020.07.008.
Sedlak J, Lindsay RH. 1968. Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Anal Biochem 25: 192-205. DOI: 10.1016/0003-2697(68)90092-4.
Senn O, Russi EW, Schindler C, Imboden M, von Eckardstein A, Brändli O, Zemp E, Ackermann-Liebrich U, Berger W, Rochat T, Luisetti M. 2008. Circulating alpha1-antitrypsin in the general population: Determinants and association with lung function. Respir Res 9 (1): 1-10. DOI: 10.1186/1465-9921-9-35.
Sinha AK. 1972. Colorimetric assay of catalase. Anal Biochem 47 (2): 389-394. DOI: 10.1016/0003-2697(72)90132-7.
Sun M, Zigman S. 1978. An improved spectrophotometric assay for superoxide dismutase based on epinephrine autoxidation. Anal Biochem 90 (1) 81-89. DOI: 10.1016/0003-2697(78)90010-6.
Szabo G, Saha B. 2015. Alcohol’s effect on host defense. Alcohol Res Curr Rev 37 (2): 159.
Teschke R. 2019. Alcoholic liver disease: Current mechanistic aspects with focus on their clinical relevance. Biomed 7 (3): 68. DOI: 10.3390/biomedicines7030068.
Weng G, Dunn W. 2019. Effect of alcohol consumption on nonalcoholic fatty liver disease. Transl Gastroenterol Hepatol 4: 70. DOI: 10.21037/tgh.2019.09.02.
Wishart DS. 2019. Metabolomics for investigating physiological and pathophysiological processes. Physiol Rev 99 (4): 1819-1875. DOI: 10.1152/physrev.00035.2018.
Xu Y, Liu H, Song L. 2020. Novel drug delivery systems targeting oxidative stress in chronic obstructive pulmonary disease: A review. J Nanobiotechnol 18 (1): 1-25. DOI: 10.1186/s12951-020-00703-5.