Electrical conductivity for seed vigor test in sorghum (Sorghum bicolor)

Abstract

Abstract. Fatonah K, Suliansyah I, Rozen N. 2017. Electrical conductivity for seed vigor test in sorghum (Sorghum bicolor). Cell Biol Dev 1: 6-12. The objectives of this study were to obtain an electrical conductivity test method for seed vigor test in sorghum, to recognize the relationship between the electrical conductivity test and potassium leakage, and to recognize the relationship between the electrical conductivity test and another variable on seed vigor. This study has two-step experiments. The objective of experiment I was to determine accurate combinations of water volume and amount of seed of the electrical conductivity test for seed vigor test in sorghum. A completely Randomized Design of 15 seed lots with 3 replications was used to determine the electrical conductivity method with different vigor. Experiment II was to test the electrical conductivity method of 21 seed lots of sorghum without an accelerated aging test, and 21 seed lots of sorghum were given an accelerated aging test. A completely Randomized Design of 21 seed lots with 3 replications was used in Experiment II. The variable observed was; standard germination, field emergence, speed of germination, first count, conductivity, potassium leakage, and eight combination conductivity method of water volume (50, 100, 150, and 200 ml) and amount of seed sorghum (50 and 75 seed count). The result of these experiments showed that the electrical conductivity test method with 150 ml water volume and 75 seed count was accurate and suitable for the sorghum seed vigor test; the electrical conductivity test showed a positive correlation with potassium leakage; and the electrical conductivity test can be used for seed vigor test in sorghum and provided the potential of physiological seed were shown through; standard germination test, field emergence test, first count test and speed of germination with negative correlation.