The endophytic bacteria producing IAA (Indole Acetic Acid) in Arachis hypogaea

Abstract

Abstract. Herlina L, Pukan KK, Mustikaningtyas D. 2017. The endophytic bacteria producing IAA (Indole Acetic Acid) in Arachis hypogaea. Cell Biol Dev 1: 31-35. Endophytic bacteria live in plant tissue and form colonies without harming the host. Every cormophyte with a stem and root may contain some endophytic bacteria that can produce biological compounds or secondary metabolites. The objective of the study was to obtain endophytic bacteria isolates from peanut plants (Arachis hypogaea) at three locations, to test in vitro the ability of endophytic bacteria isolates to produce IAA, and also to analyze IAA on the growth and development of mungbean plants. The study was carried out in three stages; the first was the isolation of endophytic bacteria from the leaves, stems, and roots; the second was the test of in vitro endophytic bacteria isolates to understand the ability of IAA production. IAA assay was measured using a spectrophotometer with a wavelength of 535 nm; the third was the introduction of IAA-producing endophytic bacteria in mungbean. The parameters observed were the length of sprouts and the number of lateral roots. The results showed that 16 isolates were selected based on IAA-producing ability. The isolates could produce different IAA with different morphological characteristics. After the fourth day of incubation, the highest and lowest IAA amounts were 69.68 (mg L^-1) and 8.50 (mg L^-1), respectively. Isolates that produce high IAA levels are applied to mungbean; it affects the number of lateral roots but does not affect the length of the sprouts. DM and K1K1 isolates have the effect of increasing lateral root formation and are expected to be potential sources of bioactive metabolites.