Optimization of L-asparaginase production from endophytic bacteria isolated from the mangrove Rhizophora mucronata

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DOI https://doi.org/10.13057/nusbiosci/n150215
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Vol. 15 No. 2 (2023)
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Articles

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ARIN NAFISATURRAHMAH
Department of Bioscience, Graduate Program, Faculty of Mathematics and Natural Sciences, Universitas Sebelas Maret. Jl. Ir. Sutami 36A, Surakarta 57126, Central Java, Indonesia
ARI SUSILOWATI ♥
Department of Bioscience, Graduate Program, Faculty of Mathematics and Natural Sciences, Universitas Sebelas Maret. Jl. Ir. Sutami 36A, Surakarta 57126, Central Java, Indonesia
ARTINI PANGASTUTI
Department of Bioscience, Graduate Program, Faculty of Mathematics and Natural Sciences, Universitas Sebelas Maret. Jl. Ir. Sutami 36A, Surakarta 57126, Central Java, Indonesia
https://orcid.org/0000-0003-4541-1383

Abstract

Abstract. Nafisaturrahmah A, Susilowati A, Pangastuti A. 2023. Optimization of L-asparaginase production from endophytic bacteria isolated from the mangrove Rhizophora mucronata. Nusantara Bioscience 15: 279-287. L-asparaginase is an enzyme that hydrolyes L-asparaginase to L-aspartate and ammonia. L-asparaginase has the potential to treat acute lymphoblastic leukemia and other malignant cancers. So far, purified L-asparaginase from Escherichia coli and Erwinea chrysanthemi has been available and applied clinically in humans. However, this treatment has side effects such as allergy, cross-interaction, immune system stimulation, drug resistance, and nonspecific L-glutaminase activity. These side effects can be overcome by discovering new sources of L-asparaginase, which are serologically different but have similar therapeutic effects. This study aims to determine the optimal conditions of endophytic bacterial culture in producing L-asparaginase. Endophytic bacteria were screened using an M9 medium with asparagine as a substrate; the L-asparaginase-producing isolates showed pink zones around the colonies. Optimization of L-aparaginase production by endophytic bacteria is carried out by One Factor at A Time (OFAT). Optimization of enzyme production includes incubation time, temperature, pH, ammonium sulfate levels, and glucose concentration in the bacterial growth medium; determination of enzyme production by Nesslerization method. The results showed that 8 isolates could produce high L-asparaginase, 14 isolates had medium ability, 30 isolates had low ability, and 2 bacterial isolates did not produce L-asparaginase. Endophytic isolates were able to produce the highest L-Aparaginase under different optimal conditions. The optimal incubation time for endophytic isolates in this study was 60-84 hours, the optimal temperature was 37ºC, the optimal pH was 7, the nitrogen content was 0.25 mg/L, and the optimal glucose level was 3%.

2019-01-01

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