The initial complete chloroplast genome of Ludisia discolor in Vietnam


Viet The Ho


The medicinal plant Ludisia discolor has been highly valued for its effectiveness in treating various diseases. To better understand its genetic makeup, the complete chloroplast genome (cp) of this plant from Vietnam was sequenced, annotated, and compared with published cp genomes. The results of this study revealed that the complete cp genome of L. discolor is 151,876 bp in size consisting of 96 protein coding genes, 12 rRNA and 33 tRNA genes. Total 53 simple sequence repeats were detected in this genome, most of them are polyA and polyT. Genomic comparisons revealed significant differences between the cp genomes of L. discolor from Vietnam with other accessions from China. These findings could provide valuable insights for the taxonomy, plant identification, breeding, and conservation programs of L. discolor in Vietnam.



Amar MH, Magdy M, Wang L, Zhou H, Zheng B, Jiang X, Atta AH, Han Y 2019. Peach chloroplast genome variation architecture and phylogenomic signatures of cpDNA introgression in Prunus. Can J Plant Sci 99(6): 885-896.
Beier S, Thiel T, Münch T, Scholz U, Mascher M 2017. MISA-web: a web server for microsatellite prediction. Bioinformatics 33, 2583–2585.
Kang Y, Deng Z, Zang R, Long W 2017. DNA barcoding analysis and phylogenetic relationships of tree species in tropical cloud forests. Sci Rep 7: 12564.
Katoh K, Rozewicki J, Yamada KD 2019. MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Brief Bioinform 20(4): 1160–1166.
Lei W, Ni D, Wang Y, Shao J, Wang X, Yang D, Wang J, Chen H, Liu C 2016. Intraspecific and heteroplasmic variations, gene losses and inversions in the chloroplast genome of Astragalus membranaceus. Sci Rep 6: 21669.
Nazarov V, Semenova E, Andronova E, Tsokalo I, Semenova E 2023. Development of clonal micropropagation technology for Ludisia discolor (Ker Gawl.) a. rich. In vitro conditions. BIO web of conferences 53, 03003.
Oh SH, Suh HJ, Park J, Kim Y, Kim S 2019. The complete chloroplast genome sequence of Goodyera schlechtendalianain Korea (Orchidaceae). Mitochondrial DNA B 4(2): 2692-2693).
Poobathy R, Zakaria R, Murugainyah V, Subramaniam S 2019. Surface sterilization and micropropagation of Ludisia discolor. Biocatal. Agric. Biotechnol 22.
Shiau YJ, Sagare AP, Chen UC, Yang SR, Tsay HS 2002. Conservation of Anoectochilus formosanus Hayata by artificial cross-pollination and in vitro culture of seeds. Bot Bull Acad Sin 43(2):123–130.
Tillich M, Lehwark P, Pellizzer T, Ulbricht-Jones ES, Fischer A, Bock R and Greiner S 2017. GeSeq – versatile and accurate annotation of organelle genomes. Nucleic Acids Res 45: W6-W11.
Yu X, Xie TX, Liu B, Zhang DY, Liu XD, Ai Y, Liu ZJ 2019. The complete chloroplast genome sequence of Ludisia discolor from Hainan of China. Mitochondrial DNA B, 4:2, 3663-3664.