Callus and shoot induction of leaf culture Lilium longiflorum with NAA and BAP
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Abstract. Lestari NKD, Deswiniyanti NW, Astarini IA, Arpiwi NLM. 2019. Callus and shoot induction of leaf culture Lilium longiflorum with NAA and BAP. Nusantara Bioscience 11: 162-165. Lilium longiflorum Thunb., an Easter lily, is a common ornamental plant used as potted plant, cut flower, cosmetic and medicine. The research on tissue culture technique to induce shoots and callus is expected to increase the yield of lilies quickly and efficiently. This study aims to determine the effect of plant growth regulator combination of Naphthalene Acetic Acid (NAA) and Benzyl Amino Purine (BAP) on the development of leaf culture and the best concentration for callus induction and shoots of lily plants. This study was conducted in a completely randomized design using leaf explants in Vacin and Went medium, with combination of NAA and BAP (0; 0.5; 1 mg..L-1 ) as the treatments. The result of the eight-week observations shows that the plant growth regulators significantly affected either in days initiation callus, days initiation of shoot, percentage of callus, percentage of shoot, diameter of callus, no of shoot and length of shoot. The combination of 1 mg.L-1 NAA and BAP had the best effect in the percentage of explants forming shoots (100%), means number of shoots (5.8), and means length of shoot (11.6 cm).
2019-01-01
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References
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Chang C, Chen CT, Ching Tsai Y, Chang WC. 2000. A Tissue culture protocol for propagation of a rare plant LiliumspeciosumThunb.var. gloriosoides Baker. Bot. Bull. Acad. Sin. 41: 139- 142.
Deswiniyanti, N. W., Astarini, I. A., and Puspawati, N. M. 2012. Phenological Study of Lilium longiflorumThunb Inflorescence.Metamorfosa Journal of Biological Sciences, 1 (1) .6-10.
Doung T.N. 2003. The control of in vitro direct main stem formation of Liliumlongiflorum derived from receptacle culture and rapid propagation by using in vitro stem nodes. Plant Growth Regul. 40: 179-184.
Directorate General of Horticulture. 2014.Income and expenditure data horticultural crop seeds in 2014.The Directorate General of Horticulture. Jakarta
Gomez K.A. and Gomez A.A. 1984. Statistical procedures for agricultural research. John Wiley & Sons, New York, pp. 328-332.
Han B.H., Yu H.J., Yae B.W., Peak K.Y. 2004. In vitro micropropagation of Liliumlongiflorum ‘Georgia’ by shoot formation as influenced by addition of liquid medium. SCI. HORT. 103: 39-49
Hicks G. 1994. Shoot induction and organogenesis in vitro: a developmental perspective. In Vitro Cell Dev. Biol-Plant. 301:10-15.
Irawati. 2005. Callus formation and embryogenesis of petiole and leaf cultures of Caladium hybrid. BeritaBiologi. 7(5): 257-261.
Kim M.S., Jeon, J.H., Youm, J.W., Kim J.H., Lee B.C., Kang W.J., Kim H.S., Joung H. 2005. Efficient plantlet regeneration via callus induction from leaf explants in Lilium oriental hybrid ‘Casa Blanca’. J. Plant Biotech. 7: 129-134.
Kumar S., Awasthi V., Kanwar J.K. 2007. Influence of growth regulators and nitrogenous compounds on in vitro bulblet formation and growth in oriental lily. HORT. SCI. 34: 77-83.
Kristina, N.N. 2009. Induction of Tabatobarito (Ficusdeltoidea Jack) shoots in vitro using Benzyl Adenine (BA) and Naphthalene Acetic Acid (NAA). Litri Journal Bogor Research Institute for Medicinal and Aromatic Plants 15 (1): 33-39.
Lestari, N. K.D, Astarini, I. A., and Oka Nurjaya, I. G. M. 2012.Changes in Stomata Anatomy of Trumpet Lili Leaves (Liliumlongiflorum) After X-ray Radiation Exposure.Metamorfosa Journal of Biological Sciences, 1 (1) .1-5.
Loretta B., Remotti C.,Bernardini C.,Saccardo F. 2003. Adventitious shoot regeneration from leaf explants and stem nodes of Lilium. Plant Cell, Tissue and Organ Culture 74 (1):37-44.
Ling-Fei, X., FengWang, M. and Dong, L. 2009.Plant regeneration from in vitro culturedleaves of Lanzhou lily (Liliumdavidii var. unicolor). Scientia Horticulturae199:458-461.
Min Ji K., Ramzan F., Yun-Jae, Yoon-Jung A., Yunim H., Chang-Kil K., and Younis K.A. 2017.Chromosomal analysis of Liliumlongiflorum x Asiatic hybrids using GISH (genomic in situ hybridization). Horticulture, Environment, and Biotechnology. 58(6): 591-600.
Mir, J.I., Ahmed, N., Itoo, H., Sheikh, M.A., Rizwan R. and Shabir , H .W. 2012. In vitro propagation of Lilium (Liliumlongiflorum). Indian Journal of Agricultural Sciences 82 (5): 455–458.
Naing A.N. , Yuna H. , Lucidosa J. , Hwanga Y.J , Kima K.M , Ahnb B.J and Lima K.B. 2014. Plant regeneration through various explants of Lilium longiflorum hybrid “Bright Tower” and determinationof ploidy level of regenerated plants. Plant Biosystems 148 (2): 191-199.
Pandey.R.K, Singh A.K and Sharma M. 2009. In Vitro Propagation of Lilium.An International Journal 1(2): 26-28.
Prihantini AI, Sukito A, and Tachibana S. 2018.Production of antioxidant compound from tissue culture of Artemisia annua. Nusantara Bioscience 10: 251-255.
Ranjana K.,Kumar S., Kanwar J.K.and Mahajan P.K. 2008. In Vitro Bulblet Productivity In DifferentExplants Of Hybrid Lilies. Journal of Fruit and Ornamental Plant Research 16:345-352.
Reinhardt D, MandelT and Kuhlemeier C, 2000. Auxin Regulates the Initiation and Radial Position of Plant Lateral Organs. Plant Cell 12(4), 507-518.
Satiew, K and Umamanit T. 2015. Micropropagation of Lilium formolongo via Leaf Explants. International Journal of Agricultural Technology 11(4):855-862.
SajidG.M.,Kaukab M., and Ahmad Z. 2009. Foliar Application Of Plant Growth Regulators (PGR) And Nutrients For Improvement Of Lily Flowers.Pak. J. Bot., 41(1): 233-237.
Sari, H.S., Murni D., Iman B. 2015. The effects of NAA and BAP on the formation of shoots, leaves, andHeight Micro cuttings shootsNepenthes ampullaria Jack.Biosfera 32 (3). 194-201.
Santoso, U., and Fatimah N. 2004.Tissue Culture. Ed. 2.UMM Press. Malang
ShofiyaniA., and Hajoeningtijas. 2010. Influence Of Sterilan And Time Of Recording On Explants Of Kencur Leaf (Kaemfseria galanga L) For Improving The Success Of Turn Culture. Agritech 12:11-29.
Tan Nhut D, Van Le B., Tanaka M., and Thanh Van K.R. 2001. Shoot induction and plant regeneration from receptacle tissue of Liliumlongiflorum. Scientia Horticulture 87: 131- 138.
Yashinta, R.E.W., and Menwangi.N.H. 2019. Micropropagation of Moruscathayanathrough in vitro culture from local Bogor, West Java, Indonesia. Nusantara Bioscience. 11(1): 18-22.
Yusnita, Budi S., Agus K., and Dwi H. 2017. Organogenesis in Melinjo Leaf Explants (Gnetumgnemon L.)In Vitro asResponse to Benzyladenine (BA) and Naphthalenesetic Acid (NAA).BKSPTNB Conference pangkalpinang
Wardani, D.P., Solichatum, Ahmad, D.S. 2004.Growth and Production of Saponin Culture of Callus Talinumpaniculatum Gaertn to added variation 2,4- Diklorofenoksi Asetat (2,4-D) and Kinetin. Biofarmasi. 2 (1): 35-43
Wattimena, G. A., Gunawan, L. W., Mattjik, N. A., Syamsudin, E., Wiendi, N. M. A., and Ernawati, A., 1992. Biotechnology Plant. Bogor: Agricultural Institute Bogor.
Chang C, Chen CT, Ching Tsai Y, Chang WC. 2000. A Tissue culture protocol for propagation of a rare plant LiliumspeciosumThunb.var. gloriosoides Baker. Bot. Bull. Acad. Sin. 41: 139- 142.
Deswiniyanti, N. W., Astarini, I. A., and Puspawati, N. M. 2012. Phenological Study of Lilium longiflorumThunb Inflorescence.Metamorfosa Journal of Biological Sciences, 1 (1) .6-10.
Doung T.N. 2003. The control of in vitro direct main stem formation of Liliumlongiflorum derived from receptacle culture and rapid propagation by using in vitro stem nodes. Plant Growth Regul. 40: 179-184.
Directorate General of Horticulture. 2014.Income and expenditure data horticultural crop seeds in 2014.The Directorate General of Horticulture. Jakarta
Gomez K.A. and Gomez A.A. 1984. Statistical procedures for agricultural research. John Wiley & Sons, New York, pp. 328-332.
Han B.H., Yu H.J., Yae B.W., Peak K.Y. 2004. In vitro micropropagation of Liliumlongiflorum ‘Georgia’ by shoot formation as influenced by addition of liquid medium. SCI. HORT. 103: 39-49
Hicks G. 1994. Shoot induction and organogenesis in vitro: a developmental perspective. In Vitro Cell Dev. Biol-Plant. 301:10-15.
Irawati. 2005. Callus formation and embryogenesis of petiole and leaf cultures of Caladium hybrid. BeritaBiologi. 7(5): 257-261.
Kim M.S., Jeon, J.H., Youm, J.W., Kim J.H., Lee B.C., Kang W.J., Kim H.S., Joung H. 2005. Efficient plantlet regeneration via callus induction from leaf explants in Lilium oriental hybrid ‘Casa Blanca’. J. Plant Biotech. 7: 129-134.
Kumar S., Awasthi V., Kanwar J.K. 2007. Influence of growth regulators and nitrogenous compounds on in vitro bulblet formation and growth in oriental lily. HORT. SCI. 34: 77-83.
Kristina, N.N. 2009. Induction of Tabatobarito (Ficusdeltoidea Jack) shoots in vitro using Benzyl Adenine (BA) and Naphthalene Acetic Acid (NAA). Litri Journal Bogor Research Institute for Medicinal and Aromatic Plants 15 (1): 33-39.
Lestari, N. K.D, Astarini, I. A., and Oka Nurjaya, I. G. M. 2012.Changes in Stomata Anatomy of Trumpet Lili Leaves (Liliumlongiflorum) After X-ray Radiation Exposure.Metamorfosa Journal of Biological Sciences, 1 (1) .1-5.
Loretta B., Remotti C.,Bernardini C.,Saccardo F. 2003. Adventitious shoot regeneration from leaf explants and stem nodes of Lilium. Plant Cell, Tissue and Organ Culture 74 (1):37-44.
Ling-Fei, X., FengWang, M. and Dong, L. 2009.Plant regeneration from in vitro culturedleaves of Lanzhou lily (Liliumdavidii var. unicolor). Scientia Horticulturae199:458-461.
Min Ji K., Ramzan F., Yun-Jae, Yoon-Jung A., Yunim H., Chang-Kil K., and Younis K.A. 2017.Chromosomal analysis of Liliumlongiflorum x Asiatic hybrids using GISH (genomic in situ hybridization). Horticulture, Environment, and Biotechnology. 58(6): 591-600.
Mir, J.I., Ahmed, N., Itoo, H., Sheikh, M.A., Rizwan R. and Shabir , H .W. 2012. In vitro propagation of Lilium (Liliumlongiflorum). Indian Journal of Agricultural Sciences 82 (5): 455–458.
Naing A.N. , Yuna H. , Lucidosa J. , Hwanga Y.J , Kima K.M , Ahnb B.J and Lima K.B. 2014. Plant regeneration through various explants of Lilium longiflorum hybrid “Bright Tower” and determinationof ploidy level of regenerated plants. Plant Biosystems 148 (2): 191-199.
Pandey.R.K, Singh A.K and Sharma M. 2009. In Vitro Propagation of Lilium.An International Journal 1(2): 26-28.
Prihantini AI, Sukito A, and Tachibana S. 2018.Production of antioxidant compound from tissue culture of Artemisia annua. Nusantara Bioscience 10: 251-255.
Ranjana K.,Kumar S., Kanwar J.K.and Mahajan P.K. 2008. In Vitro Bulblet Productivity In DifferentExplants Of Hybrid Lilies. Journal of Fruit and Ornamental Plant Research 16:345-352.
Reinhardt D, MandelT and Kuhlemeier C, 2000. Auxin Regulates the Initiation and Radial Position of Plant Lateral Organs. Plant Cell 12(4), 507-518.
Satiew, K and Umamanit T. 2015. Micropropagation of Lilium formolongo via Leaf Explants. International Journal of Agricultural Technology 11(4):855-862.
SajidG.M.,Kaukab M., and Ahmad Z. 2009. Foliar Application Of Plant Growth Regulators (PGR) And Nutrients For Improvement Of Lily Flowers.Pak. J. Bot., 41(1): 233-237.
Sari, H.S., Murni D., Iman B. 2015. The effects of NAA and BAP on the formation of shoots, leaves, andHeight Micro cuttings shootsNepenthes ampullaria Jack.Biosfera 32 (3). 194-201.
Santoso, U., and Fatimah N. 2004.Tissue Culture. Ed. 2.UMM Press. Malang
ShofiyaniA., and Hajoeningtijas. 2010. Influence Of Sterilan And Time Of Recording On Explants Of Kencur Leaf (Kaemfseria galanga L) For Improving The Success Of Turn Culture. Agritech 12:11-29.
Tan Nhut D, Van Le B., Tanaka M., and Thanh Van K.R. 2001. Shoot induction and plant regeneration from receptacle tissue of Liliumlongiflorum. Scientia Horticulture 87: 131- 138.
Yashinta, R.E.W., and Menwangi.N.H. 2019. Micropropagation of Moruscathayanathrough in vitro culture from local Bogor, West Java, Indonesia. Nusantara Bioscience. 11(1): 18-22.
Yusnita, Budi S., Agus K., and Dwi H. 2017. Organogenesis in Melinjo Leaf Explants (Gnetumgnemon L.)In Vitro asResponse to Benzyladenine (BA) and Naphthalenesetic Acid (NAA).BKSPTNB Conference pangkalpinang
Wardani, D.P., Solichatum, Ahmad, D.S. 2004.Growth and Production of Saponin Culture of Callus Talinumpaniculatum Gaertn to added variation 2,4- Diklorofenoksi Asetat (2,4-D) and Kinetin. Biofarmasi. 2 (1): 35-43
Wattimena, G. A., Gunawan, L. W., Mattjik, N. A., Syamsudin, E., Wiendi, N. M. A., and Ernawati, A., 1992. Biotechnology Plant. Bogor: Agricultural Institute Bogor.