Short Communication: Serratia rubidaea as contaminant in laboratory environment
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Abstract. Virgianti DP. 2021. Short Communication: Serratia rubidaea as contaminant in laboratory environment. Nusantara Bioscience 13: 47-51. There have been many cases of bacterial contamination in the laboratory. The bacterial genera identified as contaminants are Bacillus, Staphylococcus, Micrococcus, Pseudomonas, Shigella and Serratia. These bacteria are classified as non-pathogenic and pathogenic bacteria that can interfere with the test and potentially develop false-positive results. The present research has shown that red-colored contaminant bacteria develop in unused sterile media in our laboratory. Based on related information, Serratia marcescens is a red bacterial species that have been reported as a contaminant in the laboratory. The purpose of this study was to identifiy contaminant bacteria at the molecular level. Based on the phylogenetic characterization using the 16S rDNA gene region, this red contaminant bacterium was identified as Serratia rubidaea.
2019-01-01
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References
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Grimont F, Grimont PAD. 2006. The genus Serratia. Prokaryotes 6: 219-244. DOI: 10.1007/0-387-30746-x_11.
Immanuel G, Esakkiraj P, Jebadhas A, Lyapparaj P, Palevesam A. 2008. Investigation of Lipase Production by Milk Isolate Serratia rubidaea. Food Technol Biotechnol 46 (1): 60-65.
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Koneman EW, Allen SD, Janda WM, Schreckenberger PC, Winn WC. 5th ed. Philadelphia, Pa: Lippincott, NY; 1997. Color Atlas and Textbook of Diagnostic Microbiology pp. 417–28.
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Stock I, Burak S, Sherwood KJ, Gruger T, Wiedemann B. 2003. Natural antimicrobial susceptibilities of strains of 'unusual' Serratia species: S. ficaria, S. fonticola, S. odorifera, S. plymuthica and S. rubidaea. J Antimicrob Chemother 51(4):865-85.
Stoyanova M, Bogatzevska N. 2011. Phytopathogenic Serratia rubidaea Isolated from Tulips. Science and Technologies. 1(6): 20-24.
Salvo MD, Calcagnile M, Talà A, Tredici SM, Maffei ME, Schönrogge K, Barbero F, Alifano P. 2019. The Microbiome of the Maculinea-Myrmica Host-Parasite Interaction. Scientific Reports. 9:8048 | https://doi.org/10.1038/s41598-019-44514-7.
Indiragandhi P, Anandham R, Sa TM. 2011. Functional Significance of Insect Gut Bacteria and Their Role in Host Insect Processes, Development, and Crop Production. D.K. Maheshwari (ed.), Bacteria in Agrobiology: Plant Growth Responses Pp. 309-334. DOI 10.1007/978-3-642-20332-9_14. Springer-Verlag Berlin Heidelberg.
Al-Mijalli SHS. 2014. Isolation and characterization of plant and human pathogenic bacteria from green pepper (Capsicum annum L.) in Riyadh, Saudi Arabia. Biotech. 4:337–344. DOI 10.1007/s13205-013-0136-2.
Dundar D, Meric M, Vahaboglu H, Willke A. 2009. Pseudo-outbreak of Serratia marcescens in a tertiary care hospital. New microbiologica. 32: 273-276.
Ewing WH, Davis BR, Fife MA, Lessel EF. 1973. Biochemical characterization of Serratia liquefaciens (Grimes and Hennerty) Bascomb et al. (formerly Enterobacter liquefaciens) and Serratia rubidaea comb. nov. and designation of type and neotype strains. Int. J. Syst. Bacteriol 23:217–225.
Ghayoor M, Qadoos A, Bahadar S, Hayat A, Daud M, Hassan A, Ali F, Zeb A, Ur Rahman K, Wahab A, Khattak ZF, Khattak B. 2015. Isolation and Identification of Common Contaminants Bacteria from Working Area in Microbiology Laboratory . Journal of Bio-Molecular Sciences (JBMS). 3(2): 74-78.
Grimont F, Grimont PAD. 2006. The genus Serratia. Prokaryotes 6: 219-244. DOI: 10.1007/0-387-30746-x_11.
Immanuel G, Esakkiraj P, Jebadhas A, Lyapparaj P, Palevesam A. 2008. Investigation of Lipase Production by Milk Isolate Serratia rubidaea. Food Technol Biotechnol 46 (1): 60-65.
Karkey A, Joshi N, Chalised S, Joshi S, Shrestha S, Nguyen TNT, Dongol S, Basnyat B, Baker S, Boinett CJ. 2018. Outbreaks of Serratia marcescens and Serratia rubidaea bacteremia in a central Kathmandu hospital following the 2015 earthquakes. Trans R Soc Trop Med Hyg. 112: 467–472. doi:10.1093/trstmh/try077.
Konar J, Das S. 2013. Common Contaminants of Bacteriology Laboratory: Microbiological Paramores. International Journal of Pharmaceutical Science Invention.2(11): 2319 – 6718.
Koneman EW, Allen SD, Janda WM, Schreckenberger PC, Winn WC. 5th ed. Philadelphia, Pa: Lippincott, NY; 1997. Color Atlas and Textbook of Diagnostic Microbiology pp. 417–28.
Kumar S, Bandyopadhyay M, Chatterjee M, Mukhopadhyay P, Pal S, Poddar S, Banerjee P. 2013. Red discoloration of urine caused by Serratia rubidae: A rare case. Avicenna J Med 3(1): 20–22. doi: 10.4103/2231-0770.112790.
Kumar S, Stecher G, Tamura K. 2016. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Molecular Biology and Evolution. 33:1870-1874.
Li X, Zhu X, Wang W, Ning K. 2018. Microbial contamination screening and interpretation for biological laboratory environments. bioRxiv preprint doi: https://doi.org/10.1101/439570.
Lutpiatina L. 2015. Produk urikase dari Bacillus sp. kontaminan laboratorium. Medical Laboratory Technology Journal. 1(2): 96-101.
Menezes EA, Cezafar FC, de Sena Andrade MS, de Paula Rocha MVA, Cunha FA. 2004. Revista da Sociadade Brasileira de Medicina Tropical 37: 70-71.
Nalini S, Parthasarathi R. 2013. Biosurfactant production by Serratia rubidaea SNAU02 isolated from hydrocarbon contaminated soil and its physico-chemical characterization. 146: 619-622. DOI: 10.1016/j.biortech.2013.08.041.
Ng LSY, Teh WT, Ng SK, Eng LC, Tan TY. 2011. Bacterial contamination of hands and the environment in a microbiology laboratory. Journal of Hospital Infection. 78: 231-233. doi:10.1016/j.jhin.2011.01.025.
Okada T, Yokota E, Matsumoto I. Community acquired sepsis by Serratia rubidaea. Kansenshogaku Zasshi 2002;76(2):109–1
Priyatno TP , Dahliani YA, Suryadi Y, Samudra IM, Susilowati DN, Rusmana I, Wibowo BS, Irwan C. 2011. Identifikasi Entomopatogen Bakteri Merah pada Wereng Batang Coklat (Nilaparvata lugens Stål.). Jurnal AgroBiogen 7(2):85-95.
Siva R, Subha K, Bhakta D, Ghosh AR, Babu S. 2012. Characterization and Enhanced Production of Prodigiosin from the Spoiled Coconut. Applied Biochemistry and Biotechnology 166: 187-196. DOI: 10.1007/s12010-011-9415-8.
Stock I, Burak S, Sherwood KJ, Gruger T, Wiedemann B. 2003. Natural antimicrobial susceptibilities of strains of 'unusual' Serratia species: S. ficaria, S. fonticola, S. odorifera, S. plymuthica and S. rubidaea. J Antimicrob Chemother 51(4):865-85.
Stoyanova M, Bogatzevska N. 2011. Phytopathogenic Serratia rubidaea Isolated from Tulips. Science and Technologies. 1(6): 20-24.
Salvo MD, Calcagnile M, Talà A, Tredici SM, Maffei ME, Schönrogge K, Barbero F, Alifano P. 2019. The Microbiome of the Maculinea-Myrmica Host-Parasite Interaction. Scientific Reports. 9:8048 | https://doi.org/10.1038/s41598-019-44514-7.
Indiragandhi P, Anandham R, Sa TM. 2011. Functional Significance of Insect Gut Bacteria and Their Role in Host Insect Processes, Development, and Crop Production. D.K. Maheshwari (ed.), Bacteria in Agrobiology: Plant Growth Responses Pp. 309-334. DOI 10.1007/978-3-642-20332-9_14. Springer-Verlag Berlin Heidelberg.