Fahmi MR. 2015. Optimization of bioconversion by using mini larvae Hermetia illucens to address aquafeeds shortage. Pros Sem Nas Masy Biodiv Indon 1 (1): 139-144. The bioconversion is a natural process consisting of extracting nutrient residue from byproducts being converted into biomass of insect larvae. This is a new source of animal proteins and fats for aquaculture. The insect in the bioconversion process (Hermetia illucens, F. Stratiomyidae, O. Diptera) or Black Soldier Fly (BSF) is well known as the best biodegradation agents. The study consisted of three steps, (i) investigating the biology and life-cycle of H. illucens to collect its minilarvae (maggot), (ii) bioconversion of organic wastes such Palm Kernel Meal (PKM) using maggot, and (iii) the use of maggot as a basic source for feeding Red Gouramy (Osphronemus gouramy) (Â±60 g). The eggs of H. illucens hatched within 3 to 6 days, the larvae stage spent 3 to 4 weeks, the pre-pupae leave feeding site to drier place, the pupae reached adultâ€™s stage in about 1 weeks and the adults longevity ranged from 1 to 2 weeks. The maggot showed good ability to degrade organic waste; indicated by the nutrient content of the larvae, which contributes 45-50% and 24-30% for protein and fat respectively. The fish used in this study gained weight 300 to 400 g within five months.