Characterization of plant growth promoting rhizobacteria (PGPR) supporting organic biofertilizer

Abstract

Dewi TK, Arum ES, Imamuddin H, Antonius S. 2015. Characterization of plant growth promoting rhizobacteria (PGPR) supporting organic biofertilizer. Pros Sem Nas Masy Biodiv Indon 1 (2): 289-295. Plant Growth Promoting Rhizobacteria (PGPR) is a group of bacteria that is actively colonizing plant roots, increasing plant growth and providing protection against certain pathogens. PGPR produces plant hormones (auxin, gibberellin, and cytokinin), promotes phosphate solubilization and performs nitrogen fixation which is very important for Beyonic StarTmik organic biofertilizer. The aims of this research are to characterize PGPR potentially producing IAA (Indole-3-Acetic Acid), protease enzyme and phosphate solubilising agents that support Beyonic StarTmik organic biofertilizer. Isolated bacteria were cultured in Pikovskaya, skim milk agar (protease) and TSB (Tryptic Soy Broth) medium. The Indole 3 -Acetic Acid production was analyzed by Spectrophotometry and High-Performance Liquid Chromatography (HPLC). Seven isolates were able to produce protease enzyme, and one isolate was able to produce high amount of IAA (158.651 ppm) with 200 pp LTryptophan.