Isolation and identification of Staphylococcus aureus from subclinical infection dairy cattle in Tasikmalaya, West Java

Abstract

Herlina N, Afiati F, Cahyo AD, Herdiyani PD, Qurotunnada, Tappa B. 2015. Isolation and identification of Staphylococcus aureus from subclinical infection dairy cattle in Tasikmalaya, West Java. Pros Sem Nas Masy Biodiv Indon 1: 413-417. Subclinical mastitis is an inflammatory disease of the udder as the most considerable economic impact among dairy farmers, due to a decrease in the quantity and quality of milk. The most common cause of the udder inflammation includes microorganisms, such as E. coli, Streptococcus agalactiae and Staphylococcus aureus. The aim of this study was to isolate and identify the bacteria Staphylococcus aureus as one of the pathogenic bacteria that were often found in the incidence of mastitis. Milk sampling was randomly conducted by doing the California Mastitis Test (CMT). A positive sample of the subclinical mastitis milk then isolated using the Mannitol Salt Phenol Red Agar (MSPRA) media. Furthermore, from bacterial isolates with yellow colonies able to ferment phenol red into yellow, the Gram staining was conducted. Microscopic appearance looks round and clustered cells were then carried out using a bacterial culture Nutrient Broth (NB) for 24 hours. Results culture was then extracted to obtain bacterial DNA and PCR analysis. The results showed that the isolates from ten samples of cattle's were Staphylococcus aureus.