Biocontrol approach of controlling Aedes aegypti’s larvae using waste of carica (Vasconcellea pubescens) seed

Abstract

Supono, Sugiyarto, Susilowati A, Purwantisari S, Kurniawati FN. 2015. Biocontrol approach of controlling Aedes aegypti’s larvae using waste of carica (Vasconcellea pubescens) seed. Pros Sem Nas Masy Biodiv Indon 1: 1127-1131. Although outbreak of dengue fever frequently occurs at various regions in Indonesia yet the conventional techniques of controlling Aedes aegypti mosquito have not demonstrated significant result. So the control efforts should continue to be carried out especially using biopesticides. Waste of Carica bean (Vasconcellea pubescens (Lenne et C. Koch)) contains chemical compound named terpenoid that has the potential to be used as a biopesticide. This purpose of this study was to determine the mortality of A. aegypti mosquito larvae using carica bean waste. Samples of carica seed were collected from Dieng plateau. Dried beans were blended to make into powder. The extraction was made by maceration method using multilevel of n-hexane, ethyl acetate, and 70% ethanol. The resulted fractions were used to test the A. aegypti mosquito larvicides. The percentage of larval mortality LC50 value was calculated using Finney probit table. The results showed that the extract of Carica seeds causes the death of A. aegypti mosquito larvae at 24 and 48 hours of exposure. N-hexane fraction killed larvae of A. aegypti most effectively. The LC50 values at 24-hours and 48 hours exposure were 148.30 and 103.99 ppm respectively. In the future waste of carica seed extract can potentially be used for biocontrol of A. aegypti mosquito larvae.