Diversity of blast disease pathogens in rice plants: Isolation and characterization of the Pyricularia oryzae fungus race from West Java, Indonesia

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Vol. 9 No. 1 (2023)
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SANTOSO ♥
Balai Besar Pengujian Standar Instrumen Padi (BBPSI Padi), Badan Standardisasi Instrumen Pertanian. Jalan Raya 9 Sukamandi, Subang 41256, Jawa Barat, Indonesia
RATNA SARI DEWI
Balai Besar Pengujian Standar Instrumen Padi (BBPSI Padi), Badan Standardisasi Instrumen Pertanian. Jalan Raya 9 Sukamandi, Subang 41256, Jawa Barat, Indonesia

Abstract

Abstract. Santoso, Dewi RS. 2023. Diversity of blast disease pathogens in rice plants: Isolation and characterization of the Pyricularia oryzae fungus race from West Java, Indonesia. Pros Sem Nas Masy Biodiv Indon 9: 107-112. The fungus Pyricularia oryzae is a pathogen that causes blast disease in rice plants; this pathogen has great racial diversity and can adapt to environmental changes. This activity aims to isolate and characterize the race of P. oryzae in West Java, especially Cianjur, Sukabumi, Sumedang, and Kuningan. Isolation of the fungus P. oryzae spores based on the monoconidia method and observation of blast disease scale using SES IRRI. Race characterization was based on the Mogi method with modification, using Indonesian differential varieties consisting of Asahan, Cisokan, IR 64, Krueng Aceh, Cisadane, Cisanggarung, and Kencana Bali. The results of monoconidia isolation obtained 53 P. oryzae isolates, 17 isolates from Cianjur, 10 isolates from Sukabumi, 10 isolates from Sumedang, and 16 isolates from Kuningan. Race characterization from Cianjur obtained 8 races, namely 001, 003, 011, 021, 103, 111, 133 and 201. P. oryzae race from Sukabumi obtained 4 types, namely 001, 011, 201, and 241. P. oryzae race from Sumedang obtained 3 types, namely 001, 101, and 241. P. oryzae race from Kuningan obtained 2 types, namely 201 and 301. The racial characterization obtained 12 races, namely races 001, 003, 011, 021, 101, 103, 111, 133, 201, 241, 301 and 311. The predominant P. oryzae races were races 001 and 201. This racial composition information can be used for an effective method strategy in managing rice blast disease.

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References
Al Noman MA, Shamsi S. 2021. An improved and effective protocol for monoconidial isolation of Pyricularia oryzae. J Plant Pathol 103: 317-320. DOI: 10.1007/s42161-020-00732-x.
Anggiani N, Santoso, Sudir. 2013. Pemetaan ras blas (Pyricularia grisea) yang menyerang padi sawah di daerah Jawa Barat. In: Abdulrachman S, Pratiwi GR, Ruskandar A, Nuryanto B, Usyati N, Widyantoro, Guswara A, Samita P, Mejaya MJ (eds). Prosiding Seminar Nasional Inovasi Teknologi Adaptip Perubahan Iklim Global Mendukung Surplus 10 Juta ton Beras Tahun 2014. Badan Penelitian dan Pengembangan Pertanian Kementerian pertanian. [Indonesian]
Bhandari DR, Khanal MP, Joshi BK, Acharya P, Ghimire KH. 2017. Rice Science and Technology in Nepal 2017. Crop Development Directorate and Agronomy Society of Nepal, Nepal.
Deng Y, Zhai K, Xie Z, Yang D, Zhu X, Liu J, Wang X, Qin P, Yang Y, Zhang G, Li Q, Zhang J, Wu S, Milazzo J, Mao B, Wang E, Xie H, Tharreau D, He Z. 2017. Epigenetic regulation of antagonistic receptors confers rice blast resistance with yield balance. Sci 355: 962-965. DOI: 10.1126/science.aai8898.
Flor HH. 1971. Current status of the gene-for-gene concept. Ann Rev Phytopathol 9: 275-296. DOI: 10.1146/annurev.py.09.090171.001423.
Hayashi N, Fukuta Y. 2009. Proposal for a new international system of differentiating races of blast (Pyricularia oryzae Cavara) by using LTH monogenic lines in rice (Oryza sativa L.). JIRCAS Work Rep 63: 11-15.
Huang J, Si W, Deng Q, Li P, Yang S. 2014. Rapid evolution of avirulence genes in rice blast fungus Magnaporthe oryzae. BMC Genetics 15: 45. DOI: 10.1186/1471-2156-15-45
IRRI. 2014. Standard Evaluation System for Rice (SES). 5th edition. International Rice Research Institute, Los Banos.
Jia Y. 2009. A User-Friendly Method to Isolate and Single Spore the Fungi Magnaporthe oryzae and Magnaporthe grisea Obtained from Diseased Field Sample. Plant Health Progress 10 (1). DOI: 10.1094/PHP-2009-1215-01-BR.
Kawasaki-Tanaka A, Hayashi N, Yanagihara S, Fukuta Y. 2016. Diversity and distribution of rice blast (Pyricularia oryzae Cavara) races in Japan. Plant Dis 100 (4): 816-823. DOI: 10.1094/PDIS-04-15- 0442-RE.
Khan MAI, Ali MA, Monsur MA, Kawasaki-Tanaka A, Hayashi N, Yanagihara S, Fukuta Y. 2016 . Diversity and distribution of rice blast (Pyricularia oryzae Cavara) races in Bangladesh. J Plant Dis 100 (10): 2025- 2033. DOI: 10.1094/PDIS-12-15-1486-RE.
Lestari A, Widyastuti U, Enggarini W. 2016. Uji virulensi 100 isolat cendawan blas (Pyricularia oryzae Cavara) terhadap satu set varietas padi diferensial Indonesia. Jurnal Agrotek Indonesia 1 (1): 37-46. DOI: 10.33661/jai.v1i1.249. [Indonesian]
Mogi S, Sugandhi Z, Baskoro SW, Edwina R, Cahyadi I. 1991. Establishment of the Differential Variety Series for Pathogenic Race Identification of Rice Blast fungus and the Distribution of Race Based on the New Differential Indonesia. Rice Disease Study Grup Karawang.
Salimah NA, Kuswinanti T, Naruddin A. 2021. Eksplorasi dan penentuan ras penyebab penyakit blas padi di Kabupaten Maros. Jurnal Fitopatologi Indonesia 17 (2): 42-48. DOI: 10.14692/jfi.17.2.41-48. [Indonesian]
Santoso, Sipi S, Subiadi, Nasution A. 2019. Keragaman Ras Pyricularia grisea Penyebab Penyakit Blas pada Tanaman Padi Sawah Papua Barat. Penelitian Pertanian Tanaman Pangan 3 (1): 1-8. DOI: 10.21082/ jpptp.v3n1.2019.p1-8. [Indonesian]
Santoso, Suwarno, Nasution A, Hairmansis A, Telebanco-Yanoria MJ, Obara M, Hayashi N, Fukuta Y. 2021. Pathogenicity of isolates of the rice blast pathogen (Pyricularia oryzae) from Indonesia. Plant Dis 105: 675-683. DOI: 10.1094/PDIS-05-20-0949-RE.
Subiadi, Sipi S, Motulo HFJ. 2016. Estimasi kehilangan hasil padi akibat serangan penyakit blas leher. In: Syahbuddin H, Kindangen JG, Taulu LA, Paat PC, Josep GH, Hendayana R (eds). Prosiding Seminar Nasional Akselerasi Agroinovasi Berbasis Sumberdaya Lokal Menuju Kemandirian Pangan. Manado. Balai Besar Pengembangan dan Pengkajian Teknologi Pertanian. [Indonesian]
Sudir, Nasution A, Santoso, Nuryanto B. 2014. Penyakit blas Pyricularia grisea pada tanaman padi dan strategi pengendaliannya. Iptek Tanaman Pangan 9 (2): 85-96. [Indonesian]
Sudir, Yuliani D, Nasution A, Nuryanto B. 2013. Pemantauan penyakit utama padi sebagai dasar skrining ketahanan varietas dan rekomondasi pengendalian di beberapa daerah sentra produksi padi di Jawa. Laporan Hasil Penelitian Balai Besar Penelitian Tanaman Padi, Sukamandi. [Indonesian]
Utami DW, Amir M, Moeljopawiro S. 2000. Analisis RFLP kelompok ras dan haplotipe isolate Blas dengan DNA pelacak MGR 586. Jurnal Biologi Pertanian 5: 28-33. [Indonesian]
Wang JC, Jia Y, Wen JW, Liu WP, Liu XM, Li L, Jiang ZY, Zhang JH, Guo XL, Ren JP. 2013. Identification of rice blast resistance genes using international monogenic differentials. Crop Prot 45: 109-116. DOI: 10.1016/j.cropro.2012.11.020.
Wiyono S. 2021. Mengatasi Penyakit Blas pada Padi. Seri Dokter Tanaman IPB. https://ptn.ipb.ac.id/cms/id/berita/detail/251/seri-dokter-tanaman-ipb-mengatasi-penyakit-blas-pada-padi. [Indonesian]