Growth Hormone (GH) gene polymorphism in several Indonesian local breeds cattle
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Agung PP, Anwar A, Putra WPB, Said S. 2017. Growth Hormone (GH) gene polymorphism in several Indonesian local breeds cattle. Pros Sem Nas Masy Biodiv Indon 3: 304-308. Through the molecular genetic study, the genetic potential of individuals can be detected early so that it can be used as a parental population to improve the productivity of local cattle. The GH (Growth Hormone) gene is one of the genes that affect the productivity of animals such as growth and reproduction. The GH gene is the candidate gene in the MAS (Marker Assisted Selection) program, i.e., the selection of animals using the genetic marker for growth and carcass trait in cattle. This study aims to identify the polymorphism of the GH gene in several Indonesian local breed cattle. The local breeds cattle used in this study including the SO cattle, PO, Bali, Pesisir, and Simmental cross cattle respectively by 41, 24, 19, 8, and 31 samples. The GH gene polymorphism was detected by PCR-RFLP method using MspI restriction enzyme. The results showed that the SO, PO, and Pesisir cattle have three genotypes (AA, BB and AB) for GH gene, otherwise the Simmental cross cattle have two genotypes (BB and AB), and the Bali cattle only have one genotype (AA). Based on these results it can be concluded that the GH gene in the SO, PO, Pesisir and Simmental Cross cattle was polymorphic while the Bali cattle do not have GH gene variant (monomorphic). The information in this study can be used as the basis or foundation to start the breeding program for the Indonesia local cattle based on the molecular technology.
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References
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Erinaldi. 2014. Profil Morfologi dan pendugaan jarak genetik sapi
simmental hasil persilangan. Jurnal Ilmu Ternak dan Veteriner 19 (2):
112-122.
Anwar S, Agung PP, Wulandari AS, Sudiro A, Said S, Tappa B. 2015.
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sumba ongole (so). In: Setyawan AD, Sugiyarto, Pitoyo A, Hernawan
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Beauchemin VR, Thomas MG, Franke DE, Silver GA. 2006. Evaluation
of DNA polymorphisms involving growth hormone relative to growth
and carcass characteristics in brahman steers. Genet Mol Res 5 (3):
438-447.
Burton JL, Mcbride BW, Block E, Glimm DR, Kenelly JJ. 1994. A review
of bovine growth hormone. Can J Anim Sci 74: 167-201.
Curi RA, Palmieri DA, Suguisawa L, de Oliveira HN, Silveira AC, Lopes
CR. 2006. Growth and carcass traits associated with GH1/Alu I and
POU1F1/Hinf I gene polymorphisms in Zebu and crossbred beef
cattle. Genet Mol Biol 29 (1): 56-61.
Dekkers JCM. 2007. Marker-assisted selection for commercial crossbred
performance. J Anim Sci 85: 2104-2114.
Diwyanto K, Priyanti A, Inounu I. 2005. Prospek dan arah pengembangan
komoditas peternakan : unggas, sapi dan kambing-domba. Wartazoa
15 (1): 11-25.
Diwyanto K, Inounu I. 2009. Dampak crossbreeding dalam program
inseminasi buatan terhadap kinerja reproduksi dan budidaya sapi
potong. Wartazoa 19 (2):93-102.
Etherton TD, Bauman DE. 1998. Biology of somatotropin in growth and
lactation of domestic animals. Physiol Rev 78 (3): 745-761.
Gill JL, Stephen C, Bishop, McCorquodale C, John L. Williams JL,
Wiener P. 2010. Associations between single nucleotide
polymorphisms in multiple candidate genes and carcass and meat
quality traits in a commercial Angus-cross population. Meat Science
86: 985-993.
Glaubitz JC. 2004. Convert: a user-friendly program to reformat diploid
genotypic data for commonly used population genetic software
packages. Mol Ecol Notes 4:309-310.
Hartatik T, Volkandari SD, Rachmana MP, Sumadi. 2013. Polymorphism
leu/val of growth hormone gene identified from limousin cross local
cattle in Indonesia. Procedia Environ Sci 17: 105-108.
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Hou G-Y, Yuan Z-R, Zhou H-L, Zhang L-P, Li J-Y, Gao X, Wang D-J,
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with carcass and meat quality traits in beef cattle. Mol Biol Rep
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Jakaria, Duryadi D, Noor RR, Tappa B, Martojo H. 2007. Hubungan
polimorfisme gen hormon pertumbuhan MspI dengan bobot badan
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Agric 32 (1): 33-40.
Jakaria, Noor RR. 2011. Analysis on Alu-I growth hormone (ghAlu-I)
gene in bali cattle. J Indon Trop Anim Agric 36 (2): 77-82.
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computer program CERVUS accommodates genotyping error
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kesehatan hewan. Direktorat Jenderal Peternakan dan Kesehatan
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Marson EP, Ferraz JBS, Meirelles FV, Balieiro JCC, Eler JP, Figuerido
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composite bovine using RFLP markers. Genet Mol Res 4: 496-505.
Moiloi B, Napolinto F, Catalilo G. 2004. Genetic diversity between
Piedmontese, Meremmana and Podolica cattle breeds. J Hered 95:
250-256.
Mu’in MA. 2008. Polimorfisme Genetik Growth Hormone Dan InsulineLike Growth Factor-I Serta Efeknya Pada Pertumbuhan Prasapih Sapi
Potong Di Indonesia. [Dissertation]. Gadjah Mada University,
Yogyakarta. [Indonesian]
Nei M. 1987. Molecular Evolutionary Genetics. Columbia University
Press, New York.
Nei M, Kumar S. 2000. Molecular evolution and phylogenetics. Oxford
University Press, New York.
Paputungan U, Hakim L, Ciptadi G, Lapian HNF. 2013. Polymorphism of
growth hormone Msp1 enzyme-restriction associated with production
performance of Ongole-Crossbred cattle mated by artificial
insemination technique. J Basic Appl Sci Res 3 (6): 581-589.
Rahayu S, Sumitro SB, Susilawati T, Soemarno. 2006. Identifikasi
polimorfisme gen gh (growth hormone) sapi bali dengan metode
PCR-RFLP. Berkala Penel Hayati 12: 7-11.
Rasyid A, Romjali E, Aryogi, Pamungkas D. 2007. Evaluasi produktivitas
sapi potong persilangan dua dan tiga bangsa pada peternakan rakyat.
In: Darmono, Wina E, Nurhayati, Sani Y, Prasetyo LH,
Triwulanningsih E, Sendow I, Natalia L, Priyanto D, Indraningsih,
Herawati T (eds): Proceeding of Animal Husbandry Technology and
Veterinary National Seminar. Bogor, 21-22 August 2007.
[Indonesian]
Ribeca C, Bonfatti V, Cecchinato A, Albera A, Gallo L, Carnier P. 2014.
Effect of polymorphisms in candidate genes on carcass and meat
quality traits in double muscled Piemontese cattle. Meat Sci 96: 1376-
1383.
Sumantri C, Imron M, Sugyono, Andreas E, Misrianti R, Ishak ABL.
2011. Growth hormone gene family (gh, ghr, ghrh and pit-1)
polymorphisms and its association with superovulation response,
ovulation rate, fertilization rate and embryo quality in embryo transfer
station (bet) of cipelang. Jurnal Ilmu Ternak dan Veteriner 16 (2):
126-139.
Tambasco DD, Paz CCP, Tambasco-Studart M, Pereira AP, Alencar MM,
Freitas AR, Coutinho LL, Packer IU, Regitano LCA. 2003. Candidate
genes for growth traits in beef cattle crosses Bos taurus × Bos
indicus. J Anim Breed Genet 120 (1):51-56.
Tanabe Y, Yokohama H, Murakami J, Kano H, Tanawaki O, Okabayashi
H, Maeda Y, Koshimoto C, Nozawa K, Tumennasan K, Dashnyam B,
Zhanchiv T. 1999. Polymorphisms of the plumage colors, the skin
variations and blood proteins in the native chickens in mongolia.
Report of the Society for Researches on Native Livestock 17: 139-
153.
Yeh, FC, Boyle TJB. 1997. Population genetic analysis of co-dominant
and dominant markers and quantitative traits. Belg J Bot 129:157-16.
Erinaldi. 2014. Profil Morfologi dan pendugaan jarak genetik sapi
simmental hasil persilangan. Jurnal Ilmu Ternak dan Veteriner 19 (2):
112-122.
Anwar S, Agung PP, Wulandari AS, Sudiro A, Said S, Tappa B. 2015.
Deteksi polimorfisme gen growth hormone (gh-MspI) pada sapi
sumba ongole (so). In: Setyawan AD, Sugiyarto, Pitoyo A, Hernawan
UE, Sutomo, Widiastuti A (eds); Proceeding of Indonesian
Biodiversity Society National Seminar. Gadjah Mada University,
Yogyakarta, 21 March 2015. [Indonesian]
Beauchemin VR, Thomas MG, Franke DE, Silver GA. 2006. Evaluation
of DNA polymorphisms involving growth hormone relative to growth
and carcass characteristics in brahman steers. Genet Mol Res 5 (3):
438-447.
Burton JL, Mcbride BW, Block E, Glimm DR, Kenelly JJ. 1994. A review
of bovine growth hormone. Can J Anim Sci 74: 167-201.
Curi RA, Palmieri DA, Suguisawa L, de Oliveira HN, Silveira AC, Lopes
CR. 2006. Growth and carcass traits associated with GH1/Alu I and
POU1F1/Hinf I gene polymorphisms in Zebu and crossbred beef
cattle. Genet Mol Biol 29 (1): 56-61.
Dekkers JCM. 2007. Marker-assisted selection for commercial crossbred
performance. J Anim Sci 85: 2104-2114.
Diwyanto K, Priyanti A, Inounu I. 2005. Prospek dan arah pengembangan
komoditas peternakan : unggas, sapi dan kambing-domba. Wartazoa
15 (1): 11-25.
Diwyanto K, Inounu I. 2009. Dampak crossbreeding dalam program
inseminasi buatan terhadap kinerja reproduksi dan budidaya sapi
potong. Wartazoa 19 (2):93-102.
Etherton TD, Bauman DE. 1998. Biology of somatotropin in growth and
lactation of domestic animals. Physiol Rev 78 (3): 745-761.
Gill JL, Stephen C, Bishop, McCorquodale C, John L. Williams JL,
Wiener P. 2010. Associations between single nucleotide
polymorphisms in multiple candidate genes and carcass and meat
quality traits in a commercial Angus-cross population. Meat Science
86: 985-993.
Glaubitz JC. 2004. Convert: a user-friendly program to reformat diploid
genotypic data for commonly used population genetic software
packages. Mol Ecol Notes 4:309-310.
Hartatik T, Volkandari SD, Rachmana MP, Sumadi. 2013. Polymorphism
leu/val of growth hormone gene identified from limousin cross local
cattle in Indonesia. Procedia Environ Sci 17: 105-108.
Hartl DL, Clark AG. 1997. Principle of population genetic. Sinauer
Associates, Sunderland.
Hediger R, Johnson SE, Barendse W, Drinkwater RE, Moore SS, Hetzel J.
1990. Assignment of the GH gene locus to 19q26qter in cattle and to
11q25qter in sheep by in situ hybridization. Genomics 8: 171-174.
Hou G-Y, Yuan Z-R, Zhou H-L, Zhang L-P, Li J-Y, Gao X, Wang D-J,
Gao H-J, Xu S-Z. 2011. Association of thyroglobulin gene variants
with carcass and meat quality traits in beef cattle. Mol Biol Rep
38:4705-4708.
Jakaria, Duryadi D, Noor RR, Tappa B, Martojo H. 2007. Hubungan
polimorfisme gen hormon pertumbuhan MspI dengan bobot badan
dan ukuran tubuh sapi pesisir sumatera barat. J Indon Trop Anim
Agric 32 (1): 33-40.
Jakaria, Noor RR. 2011. Analysis on Alu-I growth hormone (ghAlu-I)
gene in bali cattle. J Indon Trop Anim Agric 36 (2): 77-82.
Kalinowski ST, Taper ML, Marshall TC. 2007. Revising how the
computer program CERVUS accommodates genotyping error
increases success in paternity assignment. Mol Ecol 16:1099-1106.
Kementerian Pertanian Republik Indonesia. 2016. Statistik peternakan dan
kesehatan hewan. Direktorat Jenderal Peternakan dan Kesehatan
Hewan, Jakarta. http://ditjenpkh.pertanian.go.id/buku-statistikpeternakan-dan-kesehatan-hewan-tahun-2016
Lawrie RA. 1995. Ilmu Daging. Parakkasi A, penerjemah; Jakarta:
Universitas Indonesia Press. Terjemahan dari: Meat Science.
Marson EP, Ferraz JBS, Meirelles FV, Balieiro JCC, Eler JP, Figuerido
LGG, Mourao GB. 2005. Genetic characterization of European-Zebu
composite bovine using RFLP markers. Genet Mol Res 4: 496-505.
Moiloi B, Napolinto F, Catalilo G. 2004. Genetic diversity between
Piedmontese, Meremmana and Podolica cattle breeds. J Hered 95:
250-256.
Mu’in MA. 2008. Polimorfisme Genetik Growth Hormone Dan InsulineLike Growth Factor-I Serta Efeknya Pada Pertumbuhan Prasapih Sapi
Potong Di Indonesia. [Dissertation]. Gadjah Mada University,
Yogyakarta. [Indonesian]
Nei M. 1987. Molecular Evolutionary Genetics. Columbia University
Press, New York.
Nei M, Kumar S. 2000. Molecular evolution and phylogenetics. Oxford
University Press, New York.
Paputungan U, Hakim L, Ciptadi G, Lapian HNF. 2013. Polymorphism of
growth hormone Msp1 enzyme-restriction associated with production
performance of Ongole-Crossbred cattle mated by artificial
insemination technique. J Basic Appl Sci Res 3 (6): 581-589.
Rahayu S, Sumitro SB, Susilawati T, Soemarno. 2006. Identifikasi
polimorfisme gen gh (growth hormone) sapi bali dengan metode
PCR-RFLP. Berkala Penel Hayati 12: 7-11.
Rasyid A, Romjali E, Aryogi, Pamungkas D. 2007. Evaluasi produktivitas
sapi potong persilangan dua dan tiga bangsa pada peternakan rakyat.
In: Darmono, Wina E, Nurhayati, Sani Y, Prasetyo LH,
Triwulanningsih E, Sendow I, Natalia L, Priyanto D, Indraningsih,
Herawati T (eds): Proceeding of Animal Husbandry Technology and
Veterinary National Seminar. Bogor, 21-22 August 2007.
[Indonesian]
Ribeca C, Bonfatti V, Cecchinato A, Albera A, Gallo L, Carnier P. 2014.
Effect of polymorphisms in candidate genes on carcass and meat
quality traits in double muscled Piemontese cattle. Meat Sci 96: 1376-
1383.
Sumantri C, Imron M, Sugyono, Andreas E, Misrianti R, Ishak ABL.
2011. Growth hormone gene family (gh, ghr, ghrh and pit-1)
polymorphisms and its association with superovulation response,
ovulation rate, fertilization rate and embryo quality in embryo transfer
station (bet) of cipelang. Jurnal Ilmu Ternak dan Veteriner 16 (2):
126-139.
Tambasco DD, Paz CCP, Tambasco-Studart M, Pereira AP, Alencar MM,
Freitas AR, Coutinho LL, Packer IU, Regitano LCA. 2003. Candidate
genes for growth traits in beef cattle crosses Bos taurus × Bos
indicus. J Anim Breed Genet 120 (1):51-56.
Tanabe Y, Yokohama H, Murakami J, Kano H, Tanawaki O, Okabayashi
H, Maeda Y, Koshimoto C, Nozawa K, Tumennasan K, Dashnyam B,
Zhanchiv T. 1999. Polymorphisms of the plumage colors, the skin
variations and blood proteins in the native chickens in mongolia.
Report of the Society for Researches on Native Livestock 17: 139-
153.
Yeh, FC, Boyle TJB. 1997. Population genetic analysis of co-dominant
and dominant markers and quantitative traits. Belg J Bot 129:157-16.