The effect of Laser Assisted Hatching size on mice's (Mus musculus) of early blastocyst viability post vitrification as a rare endangered conservation effort

Abstract

Gunawan M, Kaiin EM, Andini DS, Anggraini CMP, Haifa R, Widyawati Z, Setiorini. 2017. The effect of Laser Assisted Hatching size on mice's (Mus musculus) of early blastocyst viability post vitrification as a rare endangered conservation effort. Pros Sem Nas Masy Biodiv Indon 3: 376-380. The aim of this study was to find out that the zona thinning of embryo with Laser Assisted Hatching can assist in the viability of embryo culture post vitrification. The embryo test used in the study was early blastocyst post vitrification divided into five treatments (KK 1, KK 2, KP1, KP2, and KP3) with five replications. KK 1 is a normal control group that is vitrified without thinning of the zona pellucida and cultured for 72 hours, KK 2 is a treatment control group without vitrification with zona thinning of 1/2 zona pellucida and cultured for 72 hours, KP1, KP2, and KP3 are blastocyst treatment groups a vitrified and thinning of pellucid zone treatment of each 1/4 of the of the pellucid zone, 1/2 of the pellucid zone and 2/3 of the pellucid zone in succession. Based on the results of the research, the percentage of viability KK 1, KK 2, KP1, KP2, and KP3 are respectively 68.33%; 80.00%; 66.67%; 78.33%; 65.00. The results of this study indicate that the thinning 1/2 of the zona pellucida (KP2) is the most effective measure to help improve the development of early blastocyst culture and viability as it approximates embryonic development in KK 2.