In vitro shoot induction from intact protocorm and early stage development in Dendrobium phalaenopsis
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Setiaji A, Setiari N, Semiarti E. 2018. In vitro shoot induction from intact protocorm and early stage development in Dendrobium phalaenopsis. Pros Sem Nas Masy Biodiv Indon 4: 20-27. Anggrek larat (Dendrobium phalaenopsis Fitzg.) is the identity flora of Moluccas Province, Indonesia. Based on Government Regulation no. 7 of 1999 on the preservation of plant and animal species, this orchid belongs to 12 species of protected orchids. In vitro propagation needs to be done as a conservation and fulfillment of market needs. Seeds are grown in Vacin and Went (VW) medium-enriched 1.0, 2.0, 3.0 g l-1 peptone. The characteristics of each of the developing phases in the germinated seed were observed and analyzed. For the establishing of proliferating cultures, intact protocorm of 2.7 months of age were growing on Murashige and Skoog (MS) medium with the addition of 4.54, 9.08, 13.62 mM Thidiazuron (TDZ) and 4.44, 13.32, 17.76, 26.64 mM 6-Benzylaminopurine (BAP), and control. The initial phase of development of seeds is divided into 6 phases that optimally grow on VW media addition of peptone 2.0 g l-1. Based on the value of the bud formation capacity (BFC) index, the optimal addition of TDZ 9.08 mM was used for shoot induction in protocorm of D. phalaenopsis with value 1.83±0.59.
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References
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Chen JT, Chang WC. 2002. Effects of tissue culture conditions and
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Chen JT and Chang WC. 2004. Induction of repetitive embryogenesis
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Germination of mature seeds of Calanthe tricarinata Lindl., an
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Cell Dev Biol Plant 46: 323-328.
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vitro multiplication of Cymbidium pendulum (Roxb.) Sw. Hort Sci
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Biotechnol 18 (1): 217-220.
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Cypripedium formosanum in relation to seed germination in vitro. J
Amer Soc Hort Sci 130: 747-753
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changes in endogenous abscisic acid concentrations and asymbiotic
seed germination of a terrestrial orchid, Calanthe tricarinata Lindl J
Amer Soc Hort Sci 132: 246-252.
Mathur G, Nadgauda RS. 1998. In vitro plantlet regeneration of blue pine
(Pinus wallichiana A.B. Jacks) In: National Symposium on
Commercial Aspects of Tissue Culture, Molecular. Biology and
Biotechnology of Medicinal Plants, Jamia Hamdard University, New
Delhi.
Molnár Z, Virág E, Ördög V. 2011. Natural substances in tissue culture
media of higher plants. Acta Biologica Szegediensis 55 (1): 123-127.
Mose W, Indrianto A, Purwantoro A, Semiarti E. 2017. The influence of
thidiazuron on direct somatic embryo formation from various types of
explant in Phalaenopsis amabilis (L.) Blume orchid. Hayati J Biosci
24 (4): 201-205.
Murashige T, Skoog F. 1962. A revised medium for rapid growth and
bioassays with tobacco tissue cultures. Physiol Plant 15: 473-497.
Nambiar N, Tee CS, Maziah M. 2012. Effects of organic additives and
different carbohydrate sources on proliferation of protocorm-like
bodies in Dendrobium alya Pink. Plant Omics J 5 (1): 10-18.
Ng C-Y, Saleh NM, Zaman FQ. 2010. In vitro multiplication of the rare
and endangered slipper orchid, Paphiopedilum rothschildianum
(Orchidaceae). African J Biotechnol 9 (14): 2062-2068.
Nhut DT, Thi NN, Bui TKL and Luan VQ. 2008. Peptone stimulates in
vitro shoot and root regeneration of avocado (Persea americana
Mill.). Scientia Horticulturae 115: 124-128.
Ningrum EFC, Rosyidi IN, Puspasari RR, Semarti E. 2017.
Perkembangan awal protocorm anggrek Phalaenopsis amabilis secara
in vitro setelah penambahan zat pengatur tumbuh ?-naphtaleneacetic
acid dan thidiazuron. Biosfera 34 (1): 9-14.
Novero M, Genre A, Szczyglowski K, Bonfante P. 2008. Root hair
colonization by mycorrhizal fungi. In: Plant Cell Monographs.
Springer, Berlin, Heidelberg
Parthibhan S, Rao MV, Kumar TS. 2015. In vitro regeneration from
protocorms in Dendrobium aqueum Lindley - An imperiled orchid. J
Genet Eng Biotechnol 13 (2): 227-233.
Peraturan Pemerintah No. 7 tahun 1999 tentang Pengawetan Jenis
Tumbuhan dan Satwa
Ruzin SE. 1999. Plant Microtechnique and Microscopy. Oxford
University Press, Oxford.
Saborio F, Dvorak WS, Donahue JK, Thorpe TA. 1997. In vitro
regeneration of plantlets from mature embryos of Pinus ayacayuite.
Tree Physiol 17 : 787-796.
Su YH, Liu YB, Zhang XS. 2011. Auxin-cytokinin interaction regulates
meristem development. Mol Plant 4: 616-625.
Vacin E, Went FW. 1949. Some pH changes in nutrient solutions. Bot
Gaz 110: 605-613.
Vinogradova T and Andronova EV. 2002. Chapter Four - Development of
orchid seeds and seedlings. In: Orchid Biology: Reviews and
Perspectives VIII: Kluwer Academic Publishers, Nederland.
Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling
T. 2003. Cytokinin-deficient transgenic Arabidopsis plants show
multiple developmental alterations indicating opposite functions of
cytokinins in the regulation of shoot and root meristem activity. Plant
Cell 15: 2532-2550.
Yamazaki J, Miyoshi K. 2006. In vitro asymbiotic germination of
immature seed and formation of protocorm by Cephalanthera falcata
(Orchidaceae). Ann Bot 98: 1197-1206.
Yoshiki Y, Kanai D, Awai K, Shimojima M, Masuda T, Shimada H,
Takamiya K, Ohta H. 2003. Light and cytokinin play a co-operative
role in MGDG synthesis in greening cucumber cotyledons. Plant Cell
Physiol 44 (8): 844-855.
New York.
Aremu AO, Bairu MW, Doležal K, Finnie JF, Van Staden J. 2012.
Topolins: a panacea to plant tissue culture challenges? Plant Cell Tiss
Organ Cult 108: 1-16.
Belyaeva OB and Litvin FF. 2007. Photoactive pigment-enzyme
complexes of chlorophyll precursor in plant leaves. Biochemistry 72:
1458-1477.
Charuvi D, Kiss V, Nevo R, Shimoni E, Adam Z, Reich Z. 2012. Gain and
loss of photosynthetic membranes during plastid differentiation in the
shoot apex of arabidopsis. Plant Cell 24: 1143-1157.
Chawla HS. 2009. Introduction to Plant Biotechnology. Oxford and IBH
Publishing Company Pvt, New Delhi.
Chen JT, Chang WC. 2002. Effects of tissue culture conditions and
explant characteristics on direct somatic embryogenesis in Oncidium
‘Gower Ramsey’. Plant Cell Tiss Organ Cult 69: 41-44.
Chen JT and Chang WC. 2004. Induction of repetitive embryogenesis
from seed-derived protocorms of Phalaenopsis amabilis var. formosa
Shimadzu. In vitro Cell Dev Biol Plant 40 (3): 290-293.
David D, Gansau JA, Abdullah JO. 2008. Effect of NAA and BAP on
protocorm proliferation of Borneo Scented Orchid, Vanda helvola.
AsPac J Mol Biol Biotechnol 16 (3): 221-224.
Dutra D, Kane ME, Richardson L. 2009. Asymbiotic seed germination
and in vitro seedling development of Cyrtopodium punctatum: a
propagation protocol for an endangered Florida native orchid. Plant
Cell Tiss Organ Cult 96: 235-243.
Eibl R, Werner S, Eibl D. 2009. Disposable bioreactors for plant liquid
cultures at Litre-scale. Eng Life Sci 9: 156-164.
Ernst R. 1994. Effects of thidiazuron on in vitro propagation
of Phalaenopsis and Doritaenopsis (Orchidaceae). Plant Cell Tiss
Organ Cult. 39: 273-275.
Feng JH, Chen JT. 2014. A Novel In vitro protocol for inducing direct
somatic embryogenesis in Phalaenopsis aphrodite without taking
explants. Sci World J. 2014: 263642. DOI: 10.1155/2014/263642.
Gnasekaran P, Poobathy R, Mahmood M, Samian MR, Subramaniam S.
2012. Effects of complex organic additives on improving the growth
of PLBs of Vanda Kasem’s Delight. Aus J Crop Sci 6 (8): 1245-1248.
Godo T, Komori M, Nakaoki E, Yukawa T, Miyoshi K, 2010.
Germination of mature seeds of Calanthe tricarinata Lindl., an
endangered terreserial orchid, by asymbiotic culture in vitro. In vitro
Cell Dev Biol Plant 46: 323-328.
Gunawan LW. 1995. Teknik Kultur Invitro dalam Hortikultura. Penebar
Swadaya, Depok.
Hajong S, Kumaria S, Tandon P. 2013. Effect of plant growth regulators
on regeneration potential of axenic nodal segments of Dendrobium
chrysanthum Wall. ex Lindl. J Agric Sci Tech 15: 1425-1435.
Hossaina MM, Sharma M, da Silva JAT, Pathak P. 2010. Seed
germination and tissue culture of Cymbidium giganteum Wall. ex
Lindl. Scientia Horticulturae 123 (4): 479-487.
Hsu RC-C, Lee YI. 2012. Seed development of Cypripedium debile Rchb.
f. in relation to asymbiotic germination. Hortscience 47 (10): 1495-
1498.
Ioio RD, Linhares FS, Sabatini S. 2008. Emerging role of cytokinin as a
regulator of cellular differentiation. Curr Opin Plant Biol 11 (1): 23-
27.
Kamada-Nobusada T, Makita N, Kojima M, Sakakibara H. 2013.
Nitrogen-dependent regulation of de novo cytokinin biosynthesis in
rice: The role of glutamine metabolism as an additional signal. Plant
Cell Physiol 54 (11): 1881-1893.
Kaur S, Bhutani KK. 2012. Organic growth supplement stimulants for in
vitro multiplication of Cymbidium pendulum (Roxb.) Sw. Hort Sci
(Prague) 39: 47-52.
Kauth PJ, Vendrame WA, Kane ME. 2006. In vitro seed culture and
seedling development of Calopogon tuberosus. Plant Cell Tissue
Organ Cult 85: 91-102.
Kiba T, Kudo T, Kojima M, Sakakibara H. 2011. Hormonal control of
nitrogen acquisition: roles of auxin, abscisic acid, and cytokinin. J
Exp Bot 62: 1399-1409.
Latip MA, Rosmah M, Zaleha A, Ting LH, Govindasamy LM, Ripin R.
2010. Effects of N6-benzyladenine and thidiazuron on proliferation of
Phalaenopsis gigantea protocorms. Asia Pacific. J Mol Biol
Biotechnol 18 (1): 217-220.
Lee YI, Lee N, Yeung EC, Chung MC. 2005. Embryo development of
Cypripedium formosanum in relation to seed germination in vitro. J
Amer Soc Hort Sci 130: 747-753
Lee YI, Lu CF, Chung MC, Yeung EC, Lee N 2007 Developmental
changes in endogenous abscisic acid concentrations and asymbiotic
seed germination of a terrestrial orchid, Calanthe tricarinata Lindl J
Amer Soc Hort Sci 132: 246-252.
Mathur G, Nadgauda RS. 1998. In vitro plantlet regeneration of blue pine
(Pinus wallichiana A.B. Jacks) In: National Symposium on
Commercial Aspects of Tissue Culture, Molecular. Biology and
Biotechnology of Medicinal Plants, Jamia Hamdard University, New
Delhi.
Molnár Z, Virág E, Ördög V. 2011. Natural substances in tissue culture
media of higher plants. Acta Biologica Szegediensis 55 (1): 123-127.
Mose W, Indrianto A, Purwantoro A, Semiarti E. 2017. The influence of
thidiazuron on direct somatic embryo formation from various types of
explant in Phalaenopsis amabilis (L.) Blume orchid. Hayati J Biosci
24 (4): 201-205.
Murashige T, Skoog F. 1962. A revised medium for rapid growth and
bioassays with tobacco tissue cultures. Physiol Plant 15: 473-497.
Nambiar N, Tee CS, Maziah M. 2012. Effects of organic additives and
different carbohydrate sources on proliferation of protocorm-like
bodies in Dendrobium alya Pink. Plant Omics J 5 (1): 10-18.
Ng C-Y, Saleh NM, Zaman FQ. 2010. In vitro multiplication of the rare
and endangered slipper orchid, Paphiopedilum rothschildianum
(Orchidaceae). African J Biotechnol 9 (14): 2062-2068.
Nhut DT, Thi NN, Bui TKL and Luan VQ. 2008. Peptone stimulates in
vitro shoot and root regeneration of avocado (Persea americana
Mill.). Scientia Horticulturae 115: 124-128.
Ningrum EFC, Rosyidi IN, Puspasari RR, Semarti E. 2017.
Perkembangan awal protocorm anggrek Phalaenopsis amabilis secara
in vitro setelah penambahan zat pengatur tumbuh ?-naphtaleneacetic
acid dan thidiazuron. Biosfera 34 (1): 9-14.
Novero M, Genre A, Szczyglowski K, Bonfante P. 2008. Root hair
colonization by mycorrhizal fungi. In: Plant Cell Monographs.
Springer, Berlin, Heidelberg
Parthibhan S, Rao MV, Kumar TS. 2015. In vitro regeneration from
protocorms in Dendrobium aqueum Lindley - An imperiled orchid. J
Genet Eng Biotechnol 13 (2): 227-233.
Peraturan Pemerintah No. 7 tahun 1999 tentang Pengawetan Jenis
Tumbuhan dan Satwa
Ruzin SE. 1999. Plant Microtechnique and Microscopy. Oxford
University Press, Oxford.
Saborio F, Dvorak WS, Donahue JK, Thorpe TA. 1997. In vitro
regeneration of plantlets from mature embryos of Pinus ayacayuite.
Tree Physiol 17 : 787-796.
Su YH, Liu YB, Zhang XS. 2011. Auxin-cytokinin interaction regulates
meristem development. Mol Plant 4: 616-625.
Vacin E, Went FW. 1949. Some pH changes in nutrient solutions. Bot
Gaz 110: 605-613.
Vinogradova T and Andronova EV. 2002. Chapter Four - Development of
orchid seeds and seedlings. In: Orchid Biology: Reviews and
Perspectives VIII: Kluwer Academic Publishers, Nederland.
Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling
T. 2003. Cytokinin-deficient transgenic Arabidopsis plants show
multiple developmental alterations indicating opposite functions of
cytokinins in the regulation of shoot and root meristem activity. Plant
Cell 15: 2532-2550.
Yamazaki J, Miyoshi K. 2006. In vitro asymbiotic germination of
immature seed and formation of protocorm by Cephalanthera falcata
(Orchidaceae). Ann Bot 98: 1197-1206.
Yoshiki Y, Kanai D, Awai K, Shimojima M, Masuda T, Shimada H,
Takamiya K, Ohta H. 2003. Light and cytokinin play a co-operative
role in MGDG synthesis in greening cucumber cotyledons. Plant Cell
Physiol 44 (8): 844-855.