In vitro selection of rhizobacteria for control Diplodia maydis cause of ear rot disease in maize
##plugins.themes.bootstrap3.article.sidebar##
##plugins.themes.bootstrap3.article.main##
Abstract
Rahma H, Arneti, Nofrianti S. 2018. In vitro selection of rhizobacteria for control Diplodia maydis cause of ear rot disease in
maize. Pros Sem Nas Masy Biodiv Indon 4: 225-230. Maize is an important food crop in Indonesia both for consumption and for animal
feed. One of the plant-disturbing organisms in corn plants is a fungus Diplodia maydis (Barkeley) Saccardo which causes cob rot.
Biological control using rhizobacteria is one alternative for controlling D. maydis. The objective of this research was to get the
rhizobacterial of maize plant that has the ability as antagonist to D. maydis. The research was carried out in 2 stages, the first was the
ability of 16 rhizobacteria isolates to inhibit the growth of D. maydis. Second Rhizobacteria inhibitory test on D. maydis used an
experimental method with a completely randomized design using 11 treatments and 4 repetitions. Rizobacteria were selected based on
their inhibitory effect on D. maydis using the dual culture method. Activity test of bioactive compounds from rizobacterial supernatant
using disc diffusion and medium poisoning method. The results showed that isolate KJTSB 7.2 and LMTSA 5.4 have the ability as an
antagonist with inhibitory ability in dual culture method of 51.10% and 50.00%. The antibiosis test of supernatant bioactive compounds
of these isolates with disc diffusion method was 7.77 and 17.73%. Meanwhile the dry weight of D. maydis in the antibiotic test using
medium poisoning method was 0.12 g and 0.18 g with an effectiveness of 82.85% and 74.28%.
##plugins.themes.bootstrap3.article.details##
Pseudomonas sp. terhadap pertumbuhan jamur patogen Sclerotium
rolfsii Sacc. penyebab penyakit rebah semai pada tanaman kedelai.
Jurnal HPT 3 (1): 1-10.
Adriansyah A, Arri M, Hamawi M, Ikhwan A. 2015. Uji metabolit
sekunder Trichoderma sp. sebagai antimikrobia patogen tanaman
Pseudomonas solanacearum secara in vitro. Jurnal Sains Agrotech 2
(1): 25-28.
Akinsanmi OA, Mitter V, Simfendorfer S, Backhouse D, Chakraborty S.
2004. Identity and patogenicity of Fusarium spp. isolate from wheat
fields in Queensland and Northerm. Australian J Agric Res 55: 97-
107.
Bressan W, Figueiredo JEF. 2005. Biological Control of Stenocarpella
maydis in Maize Seed with Antagonistic Streptomyces sp. Isolates. J
Phytopathol 153: 623-626.
Charles WK 2009. Disease of Corn Diplodia Ear Rot.
http://wwwAgricultural.purdue.edu [13 Januari 2017].
Kristi A. 2017.Aplikasi Rizobakteri Indigenus utnuk Menekan Penyakit
Layu Stewart dan Meningkatkan Pertumbuhan Tanaman Jagung.
[Skripsi] Fakultas Pertanian. Universitas Andalas. Padang.
Laila J. 2016. Seleksi Rizobakteri Indigenos untuk Menenkan Pantoea
stewartii subsp. stewartii dan Meningkatkan Pertumbuhan Tanaman
Jagung. [Skripsi] Fakultas Pertanian. Universitas Andalas. Padang.
Liu CH, Chen X, Liu TT, Lian B, Yucheng G, Caer V, Xue YR, Wang
BT. 2007. Study antifungal activity of Actinobacter baumannii and its
antifungal componen. Appl Microbial Biotechnol 76: 459-466.
Mahartha KA, Khalimi K, Wirya GNAS. 2013. Uji efektivitas rizobakteri
sebagai agens antagonis terhadap Fusarium oxysporum f.sp. capsici
penyebab penyakit layu fusarium pada tanaman cabai rawit
(Capsicum frutescens L.). E-Jurnal Agroekoteknologi Tropika 2 (3):
145-154.
Matiello RR, Santos DDPMD, Coelho CDJ, Pria MD, Gardingo JR. 2015.
Damage in Maize Ears Associated with Methods of Inoculation of
Stenocarpella maydis. African J Agric Res 10 (28): 2711-2716.
Muharni, Fitrya M, Oktaruliza, Elfita. 2014. Uji aktivitas anti bakteri dan
anti oksidan senyawa derivat piranon dari mikroba endofitik
Penicillium sp pada tumbuhan kunyit putih (Curcuma zedoaria
(Berg.) Roscoe). Trad Med J 19 (3): 107-112.
Nofrianti S. 2018. Uji Antagonis Rizobakteri Indigenos terhadap Jamur
Diplodia maydis (Berkeley) Saccardo Penyebab Penyakit Busuk
Tongkol Pada Jagung (Zea Mays ) Secara In Vitro. [Skripsi]. Fakultas
Pertanian, Universitas Andalas, Padang.
Pal KK, Gardener MS. 2006. Biological Control of Pathogens. Plant Healt
Instructor 10: 1-25.
Parwati GAK, Khalimi CK, Adiartayasa W. 2014. Uji efikasi formulasi
rizobakteri Pantoea agglomerans GTA24 dalam mengendalikan
penyakit rebah semai yang disebabkan oleh Sceloritum rolfsii pada
tanaman kedelai. E-Jurnal Agroekoteknologi Tropika 3 (4): 218-299.
Radji M. 2011. Isolation of fungal endophytes from Garcinia mangostana
and their antibacterial activity. African J Biotechnol 10 (1): 103-107.
Rahma H, Zainal A, Suryati. 2016. Isolasi dan seleksi rizobakteri yang
berpotensi sebagai agen pengendali Pantoea stewartii subsp. stewartii
penyebab layu stewart pada tanaman jagung. Jurnal HPT Tropika 16
(2): 124-130.
Riyadi AS, Soesanto L, Kustantinah. 2008. Virulensi Fusarium
oxysporum f.sp. zingiberi isolat Boyolali dan Temanggung setelah
disimpan enam tahun dalam tanah steril. Jurnal Perlindungan
Tanaman Indonesia 14 (2): 80-85.
Rustam. 2011. Potensi Bakteri Penghasil Metabolit Sekunder untuk
Mengendalikan Penyakit Hawar Pelepah Padi yang Disebabkan oleh
Rhizoctonia solani Kunh. [Disertasi] Sekolah Pascasarjana, Institut
Pertanian Bogor, Bogor.
Sagahon IP, Reyes MAA, Rojas HVS, Cuenca AA, Jurado AT, Alvarez
IOC, Flores YM. 2011. Isolation of bacteria with antifungal activity
against the phytopathogenic fungi Stenocarpella maydis and
Stenocarpella macrospora. Intl J Mol Sci 12: 5522-5537.
Soenartiningsih. 2015. Uji Ketahanan beberapa varietas unggul jagung
terhadap penyakit Giberella dan Diplodia. Balai Penenlitian Tanaman
Serealia, Maros.
Sriyanti NLG, Suprapta DN, Suada IK. 2015. Uji keefektifan dalam
menghambat pertumbuhan cendawan Colletotrichum spp. penyebab
antraknosa pada cabai merah (Capsicum annum L.). Jurnal
Agroekoteknologi Tropika 4 (1): 2301-6515.
Syamsudin, Ulim MA. 2013. Daya hambat rizobakteri kandidat agens
biokontrol terhadap pertumbuhan koloni patogen Phytophthora
capsici secara in vitro. Jurnal Floratek 8 : 64-72.
Sutariati GAK Wahab A. 2010. Isolasi dan Uji kemampuan rizobakteri
indigenos sebagai agensia pengendalian hayati penyakit pada tanaman
cabai. Jurnal Hortikultura 20 (1): 86-95.
Tanati AE. 2012. Identifikasi Penyebab Penyakit Hawar Daun Tanaman
Buah Merah (Pandanus conoideus Lamk.) dan Pengendaliannya
Menggunakan Bakteri Rizosfer. [Tesis]. Sekolah Pasca Sarjana.
Institut Pertanian Bogor. Bogor.
Vincelli P. 1997. Ear Rot of Corn Caused by Stenocarpella maydis
(=Diplodia maydis). College of Agriculture, University of Kentucky,
USA.