Seed storage and micropropagation of pencil orchid (Papilionanthe hookeriana) in Bogor Botanic Gardens

Abstract

Abstract. Handini E. 2019. Seed storage and micropropagation of pencil orchid (Papilionanthe hookeriana) in Bogor Botanic Gardens. Pros Sem Nas Masy Biodiv Indon 5: 7-12. Papilionanthe hookeriana as “Queen of Orchid” is found in Bengkulu and Bangka Belitung as potential ornamental plants. This orchid has terete leaves and lives naturally in aquatic or semi-aquatic habitats. Seed storage at-20?C and orchid seed viability testing is intended to extend the shelf life of seeds and maintain the availability of orchid seeds. Propagation of these orchids is carried out in vitro using seeds. Seeds from broken fruit after being stored in a desiccator for five to seven days, some are placed in the freezer at-20 ?C. Orchid seeds are sterilized using 10% and 5% Clorox, then rinsed with sterile water three times. This viability test was carried out in four media, namely KC, VWS, HS, and KCA. Regeneration of Pencil Orchid PLBs using T1 and VWS media in subculture I, while subculture II used VT5, KC and ½ P. Seed viability tests at a shelf life of 26 months showed the percentage of sprouts was significantly different between HS 18, 03% and KCA 9,49%. While the percentage of sprouts in VWS 14,4% was not significantly different from KC 11,02%. Decrease in the percentage of sprouts occurs in the seed viability test the shelf life of 31 months. The results of PLBs regeneration showed that the plantlets were able to grow with VW, KC and MS base media until they were ready for acclimatization. Until now, acclimatized orchids are still alive at the LIPI Botanical Garden (a.k.a Bogor Botanic Gardens).