Antioxidant activity, total phenolic and flavonoid content of Dillenia auriculata (Dilleniaceae) bark extract

Abstract

Abstract. Liana, Murningsih T. 2018. Antioxidant activity, total phenolic and flavonoid content of Dillenia auriculata (Dilleniaceae) bark extract. Pros Sem Nas Masy Biodiv Indon 5: 129-133. Total phenolic, total flavonoid and antioxidant activity of Dillenia auriculata bark extract were determined in this study. The phenolic total was determined using Folin-Ciocalteu reagent and total flavonoid content using aluminum chloride reagent. Antioxidant activity was carried out using free radical scavenging (DPPH), cupric reducing power and ?-carotene bleaching assay. Vitamin C, vitamin E and BHT (Butylated hydroxytoluene) were used as positive control. The result showed that total phenolic content was 19.70 ± 0.05 mg GAE /g extract and total flavonoid was 19.53 ± 0.08 mg RE/g extract. The IC₅₀ value for DPPH- free radical activity was 32.28 ± 0.02 ?g/mL higher than positive control vitamin C and vitamin E, antioxidant activity by cupric reducing power on concentration 50 ?g/mL was 72,27 ± 0,03 % lower than vitamin C; ?-carotene bleaching was found 76.19 ± 0.04% lower than positive control vitamin E and BHT. The antioxidant properties may be attributed to the presence of phenolic and flavonoid compounds present in the extract.