Abstract. Saidah, Muchtar, Syafruddin, Pangestuti R. 2019. The effect of plant spacing at the growth and yield of shallot from true shallot seed in Sigi District, Central Sulawesi. Pros Sem Nas Masy Biodiv Indon 5: 209-212. Generally, shallots are cultivated using seed bulb (vegetatively). The problem is the cost of providing seed bulbs is quite high. One of the ways to save the usage of seed bulbs is by using seed (True Shallot Seed). An effort to improve the yield of shallots from the True Seed of Shallot (TSS) is the use of the right and suitable spacing. The objectives of this research were to determine the effect of various plant spacing on the growth and yield of shallots from True Seed of Shallot (TSS). This research was conducted in the farmer’s land in Kalukubula Village, Biromaru Sub-district, Sigi District, from December 2017 to March 2018. This research was designed using a Randomized Block Design non Factorial consisting of 3 treatments of spacing and repeated 10 times, so that the total experimental plot was 30 plots. The treatment consisted of JT1 = 10 cm x 10 cm, JT2 = 8 cm x 10 cm, and JT3 = 6 cm x 10 cm. The observations included plant height/length, number of leaves, number of bulbs per clump, weight of bulbs per clump, bulb weight, and bulb diameter. The results showed that the plant spacing of 8 cm x 10 cm (JT2) provided greater plant height/length, number of leaves, number of bulbs per clump, weight of bulbs per clump than the other treatments, while 10 cm x 10 cm spacing provided bulb weight and bulb diameter greater than the other treatments.