Abstract. Fitria U, Dewi TF, Wijauanti E. 2019. Analysis of liver function and kidney function in rats after giving Piper retrofractum Vahl, Plantago major and Apium graveolens. Pros Sem Nas Masy Biodiv Indon 5: 263-266. The determination of liver function values (SGOT and SGPT) and kidney function (ureum and creatinine) was one of the important aspects to assess the safety of traditional medicines. The purpose of this study was to determine the effect of giving compound of Piper retrofractum, Plantago major and Apium graveolens herbs to the liver and kidneys of rats. 30 rats were divided into 5 groups randomly. The groups were control group, treatment 1 (400 mg/200 g bw), treatment 2 (600 mg/200 g bw), treatment 3 (800 mg/200 g bw) and treatment 4 (1000 mg/200 g bw). This research was conducted for two weeks. The results of SGOT, SGPT and creatinine parameter measurements showed that there were no significant differences (p> 0.05) between all treatment groups with the control group. While in the ureum parameters, there were groups that experienced significant differences to the control group, that was the treatment group 3 (p= 0.03). However, the mean value after treatment was still below the control group (39.5 IU/L), therefore it was still within the normal range. These results indicate that the compound of Piper retrofractum Vahl, Plantago major, Apium graveolens herbs was safe for the function of the liver and kidneys of rats.