Abstract. Izati N, Zhara FA, Pertiwi RAP, Pranoto MDP, Widiyanti R, Sugiyarto. 2020. Diversity of macrofungi and their potential utilization in Mount Picis Nature Reserve and Mount Sigogor Nature Reserve, East Java. Pros Sem Nas Masy Biodiv Indon 6: 459-467. The forest condition in Mount Picis Nature Reserve and Mount Sigogor Nature Reserve is still fine and dense condition, so it is suitable for macrofungi growth. However, the diversity information of macrofungi in Mount Picis Nature Reserve and Mount Sigogor Nature Reserve in East Java is still limited. This research aims to determine the diversity of macrofungi and their potential utilization in Mount Picis Nature Reserve and Mount Sigogor Nature Reserve. This research was conducted in January-February 2019. The observation of macrofungi was performed by exploring the existed pathways on the observation site. The macrofungi were observed for morphological characteristics included shape of body, colour, shape of cap, shape of stem, lamella including ring and pore, ring, and substrates where macrofungi were found. The macrofungi were observed and documented on photographs for identification. Data were analyzed included diversity index, species wealth index, evenness index, Simpson-dominance index, and descriptively qualitative. Based on the results, it was identified 33 species from 20 families macrofungi in Mount Picis Nature Reserve with a diversity index value of 2.48 that belongs to the moderate diversity category, while in Mount Sigogor Nature Reserve it was identified 68 species from 32 families with a diversity index value of 2.78 that belongs to the moderate diversity category. Based on the potential utilization of macrofungi from both regions, 38 macrofungi species were identified as edible fungi, 25 species as medicinal fungi, 12 species as edible and medicinal fungi, 30 species as inedible fungi, and 2 species were not identified yet.