Abstract. Wardani FF, Mimin. 2020. Characterization of fruit and seeds and identification of Tacca palmata seed storage behavior using the 100-seed test method. Pros Sem Nas Masy Biodiv Indon 6: 552-557. Tacca palmata is one of species from Dioscoreaceae which has potency as a medicinal and ornamental plant. Research on the characterization of T. palmata fruits and seeds is not known yet. Though seeds are one of the reproductive material for T. palmata. In addition, seed storage is required to maintain the availability of T. palmata seeds. Information about seed storage behavior is the most important thing to know the seed storage characteristic. Therefore, the aim of the study was to provide information about the characteristic of the fruit and seeds as a basis for determining the seed storage behavior of T. palmata. The method used was 100-seeds test method. A total of 100 seeds, divided into four groups i.e. 10 seeds for initial moisture content, 26 seeds for initial germination (13 seeds per replication), 32 seeds for desiccation (6 seeds for moisture content after desiccation and 26 seeds for germination after desiccation), and 32 seeds for storage with controlled humidity (6 seeds for moisture content after storage and 26 seeds for germination after storage). The results showed that the T. palmata fruits were round and bright red and its seeds had irregularly shaped and dark brown. The water content of T. palmata after desiccation was almost the same as the initial water content (14.58%), which was 14.55% and experienced an increase after being stored with controlled humidity (36.61%). T. palmata germinated with hypogeal type. The germination rate of T. palmata after desiccation was 38.46% and not significantly different from the initial germination rate (46.67%), whereas after being stored with controlled humidity, the germination rate was doubled, i.e., 76.92%. So it can be concluded that T. palmata seeds are tolerant of desiccation (orthodox).