Abstract. Hermanasari R, Yullianida, Lestari AP, Hairmansis A. 2020. Promising rice breeding lines adapted to acidic upland area. Pros Sem Nas Masy Biodiv Indon 6: 576-585. Acid dry soil has the potential for the development of rice production to support national food security. Assembling of superior varieties of rice that are adaptive to acid dry soil is one of the keys to success in increasing rice production in these fields. The Indonesian Center for Rice Research has assembled upland rice lines that well adapted to acid dry soil, but these promising lines need advanced yield trials. This study aims to obtain well-adapted high-yielding rice lines and high-quality rice. The study was conducted in the first season of 2018 at the Tamanbogo Experimental station in Lampung. This experiment used Randomized Complete Block Design. Plant materials were 21 lines and three check varieties i.e Limboto, Situ Patenggang, and Inpago 8. The result showed that plant height ranged from 84-127 cm, the number of productive tillers ranges from 10-15 stems, the number of grains per panicle ranged from 91 to 237 grains, and the weight of 1000 grains ranged from 19.86 to 22.24 grams. Lines B15053F-PWR-3 (6.72 t ha-1), B15340-1B-TB-45 (6.04 t ha-1) and B14956-MR-2-2-2-0 (6.04 t ha-1) have higher yields than the best check Inpago 8 (5.67 t ha-1). These lines have the quality of rice with medium rice texture with amylose content ranging from 20.99-23.84%. The other tested lines were dominated by medium-length rice with a little chalkiness (LMS) and low to high amylose content ranging from 5.46-27.12% with the texture of rice varying from hard to soft. The results of testing in the greenhouse to blast disease obtained six lines resistance to blast races, two lines have tolerant to drought stress, and ten lines tolerant to Al stress.