Hematological modulatory effects of Weissella and Pediococcus sp. on formalin-induced inflammation in wistar rats

##plugins.themes.bootstrap3.article.sidebar##

PDF
Issue
Vol. 19 No. 1 (2022)
Section
Articles

##plugins.themes.bootstrap3.article.main##

BABAYEMI OLAWALE OLADEJO ♥
Department of Microbiology, Federal University of Technology. P.M.B. 704, Akure, Nigeria
HAWAH MORIYIKE OLUWASOLA
Department of Microbiology, Federal University of Technology. P.M.B. 704, Akure, Nigeria

Abstract

Abstract. Oladejo BO, Oluwasola HM. 2022. Hematological modulatory effects of Weissella and Pediococcus sp. on formalin-induced inflammation in wistar rats. Asian J Trop Biotechnol 19: 28-34. The activity of three LAB strains, Weissella cibaria II-1-59, Weissella confusa JMC 1093, and Pediococcus pentosaceus DSM20336, isolated from a Nigerian locally fermented food condiment; ‘iru’ on blood parameters of acutely inflamed rats using formalin (1%) was investigated in this study. The rats were distributed into six groups (A-F). Rats in Groups A were neither administered formalin nor treated with LAB, while Group B received formalin injection only. Groups C-E were treated with the strains of LABs (2 × 107 CFU/mL), while Group F received diclofenac sodium treatment (150 mg/kg body weight) following formalin administration. In addition, Erythrocyte sedimentation rate (ESR), total and differential white blood cell (WBC) count, and total red blood cell (RBC) count were analyzed using standard methods. LAB Treatments significantly reduced the ESR of the blood of LAB-treated rats (1.00±0.00 mm/hr at 1 hour) at P<0.05 and regulated leucocyte infiltration in the blood circulation (W. cibaria treated group with a neutrophil count of 13.67±1.20% at 336 hrs) compared to diclofenac sodium. These findings revealed that the LAB were good immune modulators and would be effective agents for the treatment of inflammation-induced anemia.

##plugins.themes.bootstrap3.article.details##

References
Aattouri N, Bouras M, Tome D, Marcos A, Lemonnier D. 2002. Oral ingestion of lactic acid bacteria by rats increases lymphocyte proliferation and interferon-? production. Br J Nutr 87 (4): 367-373. DOI: 10.1079/bjnbjn2001527.
Aboderin FI, Oyetayo VO. 2006. Hematological studies of rats fed different doses of probiotic, Lactobacillus plantarum, isolated from fermenting corn slurry. Pak J Nutr 5 (2): 102-105. DOI: 10.3923/pjn.2006.102.105.
Adeboboye CF, Oladejo BO, Adebolu TT. 2022. Effects of Lactobacillus fermentum on inflammatory-associated anaemia and leucocyte homeostasis in carrageenan-induced paw edema in wistar rats. Bact Emp 5 (1): e327. DOI: 10.36547/be.327.
Afolabi FT, Abdulkadir M, Onilude A. 2016. Isolation and screening of microorganisms associated with locust bean (Iru) for the ability to ferment soya bean to produce soy Iru. Br Microbiol Res J 13 (6): 1-10. DOI: 10.9734/BMRJ/2016/24593.
Ajayi AO. 2014. Bacteriology and Qualitative study of African locust bean (Parkia biglobosa). Open J Soc Sci 2: 73-78. DOI: 10.4236/jss.2014.211010.
Amdekar S, Roy P, Singh V, Kumar A, Singh R, Sharma P. 2012. Anti-inflammatory activity of lactobacillus on carrageenan-induced paw edema in male wistar rats. Intl J Inflam 75: 1-6. DOI: 10.1155/2012/752015.
Barcelos IP Troxel RM, Graves JS. 2019. Mitochondrial dysfunction and multiple sclerosis. Biology 8 (2): 37. DOI: 10.3390/biology8020037.
Bikheet MM, Mahmoud ME, Hassan HM, Yassien EE. 2021. The effect of two strains of LAB (P. acidilactaci and L. lactis) in laboratory rats. SJAS 3 (1): 137-143. DOI: 10.21608/sjas.2021.73876.1093.
Bray C, Bell LN, Liang H, Haykal R, Kaiksow F, Mazza JJ, Yale SH. 2016. Erythrocyte sedimentation rate and c-reactive protein measurements and their relevance in clinical medicine. WMJ 115 (6): 317-321.
Cheesborough M. 1991. Medical Laboratory Manual for Tropical Countries. 2nd Edition. Tropical Health Technology and Butterworth Scientific Limited, London.
Groeger DO, Mahony L, Murphy EF, Bourke JF, Dinan TG, Kiely B, Shanahan F, Quigley EM. 2013. Bifidobacterium infantis 35624 modulates inflammatory processes beyond the gut. Gut Microbes 4 (4): 325-339. DOI: 10.4161/gmic.25487.
Nairz M, Theurl I, Wolf D, Weiss G. 2016. Iron deficiency or anemia of inflammation? Differential diagnosis and mechanisms of anemia of inflammation. Wien Med Wochenschr 166: 411-423. DOI: 10.1007/s10354-016-0505-7.
Nemeth E, Ganz T. 2014. Anemia of inflammation. Hematol Oncol Clin North Am 28 (4): 671-681. DOI: 10.1016/j.hoc.2014.04.005.
Oladejo BO, Oluwasola HM. 2021. Oral administration of Weissella and Pediococcus sp. alleviates formalin induced inflammation in rats by cytokine modulation. Ife J Sci 23 (2): 1-10. DOI: 10.4314/ijs.v23i2.10.
Ouoba LI, Nyanga-Koumou CA, Parkouda C, Sawadogo H, Kobawila SC, Keleke S, Diawara B, Louembe D, Sutherland JP. 2010. Genotypic diversity of lactic acid bacteria isolated from African traditional alkaline-fermented foods. J Appl Microbiol 108: 2019-2029. DOI: 10.1111/j.1365-2672.2009.04603.x.
Oyetola MT, Agarry O, Oyetola S. 2017. Screening of indigenous bacterial isolates from dawadawa (African locust beans) in North Central Nigeria. Biochem Mol Biol 2(6): 67-72. DOI: 10.11648/j.bmb.20170206.11.
Paramita S, Kosala K, Dzulkifli D, Saputri DI, Wijayanti E. 2017. Anti-inflammatory activities of ethnomedicinal plants from Dayak Abai in North Kalimantan, Indonesia. Biodiversitas 18: 1556-1561. DOI: 10.13057/biodiv/d180434.
Pessione E. 2012. Lactic acid bacteria contribution to gut microbiota complexity: Lights and shadows. Front Cell Infect Microbiol 2 (86): 1-15. DOI: 10.3389/fcimb.2012.00086.
Scheiermann P, Frenette PS, Hidalgo A. 2015. Regulation of leucocyte homeostasis in the circulation. Cardiovasc Res 107 (3): 340-351. DOI: 10.1093/cvr/cvv099.
Steinbicker AU, Sachidanandan C, Vonner AJ, Yusuf RZ, Deng DY, Lai CS, Rauwerdink KM, Winn JC, Saez B, Cook CM, Szekely BA, Roy CN, Seehra JS, Cuny GD, Scadden DT, Peterson RT, Bloch KD, Yu PB. 2011. Inhibition of bone morphogenetic protein signaling attenuates anemia associated with inflammation. Blood 117 (18): 4915-4923. DOI: 10.1182/blood-2010-10-313064.
Straat M, van Bruggen R, de Korte D, Juffermans NP. 2012. Red blood cell clearance in inflammation. Transfus Med Hemother 39: 353-360. DOI: 10.1159/000342229.
Sung-Min H, Gehoon C, Yong HK, Chul-Kyu P. 2019. The role of maresins in inflammatory pain: Function of macrophages in wound regeneration. Intl J Mol Sci 20 (5849): 1-16. DOI: 10.3390/ijms20235849.
Szabo NJ, Laurie C, Dolana GA, Burdocka TS, Satob HS, Tohru U, Satoko Y, Masamichi T, Hirofumi U. 2011. Safety evaluation of Lactobacillus pentosus strain b240. Food Chem Toxicol 49 (1): 251-258. DOI: 10.1016/j.fct.2010.10.027.
Szalay J. 2018. Inflammation: Causes, Symptoms and Anti-inflammatory Diet. www.livescience.com Accessed 07/04/2020.
Tsai YT, Cheng PC, Pan TM. 2014. Anti-obesity effects of gut microbiota are associated with lactic acid bacteria. Appl Microbiol Biotechnol 98: 1-10. DOI: 10.1007/s00253-013-5346-3.
Uaboi-Egbenni PO, Okolie PN, Sobande AO, Alao O, Teniola O, Bessong PO. 2009. Identification of subdominant lactic acid bacteria in dawadawa (a soup condiment) and their evolution during laboratory-scale fermentation of Parkia biglobosa (African locust beans). Afr J Biotechnol 8 (25): 7241-7248. DOI: 10.5897/AJB2009.000-9558.
Umapathy E, Ndebia EJ, Meeme A, Adam B, Menziwa P, Nkeh-Chungag BN, Iputo JE. 2010. An experimental evaluation of Albuca setosa aqueous extract on membrane stabilization, protein denaturation and white blood cell migration during acute inflammation. J Med Plant Res 4 (9): 789-795. DOI: 10.5897/JMPR10.056.
Valentini L, Pinto A, Bourdel-Marchasson I, Ostan R, Brigidi P, Turroni S. 2015. Impact of personalized diet and probiotic supplementation on inflammation, nutritional parameters and intestinal microbiota - The “RISTOMED project”: Randomized controlled trial in healthy older people. J Clin Nutr 34: 593-602. DOI: 10.1016/j.clnu.2014.09.023.