Rubber industry waste could be degraded naturally by microorganism; especially bacteria. The ability of bacteria to degrade the waste could be used to improve waste quality. This research was purposed to isolate the bacteria from rubber industrial waste and to evaluate the ability of those bacteria in improving the quality of rubber industrial waste with indicator: pH, BOD, COD, TSS and ammonia. The bacteria were identified based on their characters of colonyâ€™s morphology, cellâ€™s morphology and physiology. Three isolated bacteria which had the most optimum growth were grew in the waste for 6 days. Quality parameters measured i.e. pH, BOD, COD, TSS and ammonia were in the day 0, 2, 4 and 6. The result showed that in rubber waste there are 18 isolated bacteria, namely BKA 1, BKA 2, BKA 3, BKA 4, BKA 5, BKA 6, BKA 7, and BKA 8. Three types of bacteria were used in the waste treatment have the different abilities to change the quality of waste. The BKA 8 was the best improving the waste of rubber industry, based on indicator of pH 8.28; BOD 58.50 mg/L; COD 87.50 mg/L; TSS 75 mg/L; and ammonia 3.17 mg/L.