Micropropagation of the orchid Dendrobium “Emma Pink” on the in vitro media culture

Abstract

An experiment of micropropagation of the orchid Dendrobium “Emma Pink†on the media Vacin and Went using tissue culture technique has been done. The explant was obtained from the young stem of Dendrobium “Emma Pinkâ€. Induction stage was started by culturing the first, second, third, fourth axilar buds counted from the base of the stem and apical bud. The most potential bud to be used as a source for an explant was identified from the serial culturing of explant on the liquid media with various combinations of growth regulator kinetin and IAA. The media were placed on a shaker at 90 rpm. The second and the third axilar buds were the most potential sources of explant at 10-5 M kinetin and 5.10-7 M IAA, with the degree of success for the formation of protocorm like bodies (protocormlike bodies) were 56.25% and 43.75% respectively. The formation and multiplication of buds were obtained from protocorm-like bodies which were cultured in the solid media in the addition of 10-4 M kinetin and 5.10-6 M IAA. The multiplication rate of the buds has continuously increased during the subcultures with an average multiplication rate of 3.25 buds/4 weeks, 4.59 buds/4 weeks, 6.32 buds/4 weeks and 8.80 buds/4 weeks respectively. The roots were formed when the buds were cultured in the solid media by adding 5.10-6 M kinetin and 10-5 M IAA. The degree of acclimatization success of Dendrobium “Emma Pink†was 93.33% for the rooted bud and 53.33% for the unrooted bud.