The effect of leaves extract of Sambiloto (Andrographis paniculata) on microanatomic structure of liver and serum glutamate pyruvate transaminase level of mice (Mus musculus) exposed to diazinon

Abstract

Diazinon is a pesticide which is often using by farmer to kill insect as the enemy of the plant. The over using of pesticide may result in the remaining of diazinon residue in farming product. This residue can cause the damage of body tissue, especially liver. The aim of research were to find out the effect of leaves sambiloto (Andrographis paniculata Ness.) extract on microanatomic structure of liver and serum glutamate pyruvate transaminase (GPT) level of mice (Mus musculus L.) exposed to diazinon. The research used Compelete Random Design with five treatments. The treatment of each group were using CMC 1% (placebo control), diazinon solution 40 mg/Kg BW (negative ontrol) and the leaves sambiloto extract 12,6; 25,2 and 37,8 mg /kg BW. Diazinon solution was given within 10 days and continued with extract of sambiloto leaves also within 10 days. Parameter observed was the microanatomic structure of liver and serum GPT level. The data was analyzed of Analysis of Varians (Anova) and continued with DMRT at significance 5%. The result of the research showed that the giving of the extract of sambiloto leaves in some dose variation degree is significantly influential to repair the microanatomic structure of liver and to decrease the serum GPT level was 37,8 mg/Kg BW.