Lipase activity and identification of lipolytic bacteria from leather industrial liquid waste in Magetan Small Industrial Area, Indonesia

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Vol. 21 No. 2 (2024)
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Articles

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JEDIDI’AH ABEL MEHOLA
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sebelas Maret. Jl. Ir. Sutami 36A Surakarta 57126, Central Java, Indonesia
RATNA SETYANINGSIH ♥
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sebelas Maret. Jl. Ir. Sutami 36A Surakarta 57126, Central Java, Indonesia
ARI SUSILOWATI
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sebelas Maret. Jl. Ir. Sutami 36A Surakarta 57126, Central Java, Indonesia

Abstract

Abstract. Mehola JA, Setyaningsih R, Susilowati A. 2024. Lipase activity and identification of lipolytic bacteria from leather industrial liquid waste in Magetan Small Industrial Area, Indonesia. Asian J Trop Biotechnol 21: 61-67. Lipolytic bacteria can produce the lipase enzyme, potentially a lipid biodegradation agent. The bacteria can be found in places that contain lots of fat, one of which is the leather industry wastewater. This research aimed to obtain lipolytic bacteria that have the potential as biodegradation agents and observe the lipase enzyme activity from small industrial lather wastewater in Magetan, Indonesia. This research was conducted in several steps, including isolation, screening, lipase enzyme activity assay, and identification of lipolytic bacteria. Isolation of lipolytic bacteria using minimal media enriched with olive oil. The screening of lipolytic bacteria was carried out using Rhodamine B agar, a method that has proven effective in such studies. The enzyme activity was determined using a spectrophotometric method, with p-nitrophenyl palmitate (p-NPP) as the substrate at ? = 410 nm. The lipolytic bacteria that exhibited high enzyme activity in each treatment were identified based on the 16S rRNA gene encoding sequence using the Basic Local Alignment Search Tool (BLAST) on the NCBI website https://www.ncbi.nlm.nih.gov/. The research showed significant findings; four isolates of lipolytic bacteria were discovered, each with unique characteristics. The highest lipase activity was observed in the form of crude enzyme. Further precipitation with ammonium sulfate revealed that isolate Q exhibited the highest enzyme activity among the other isolates in the 30-45% fraction. The 16S rRNA gene sequence analysis indicated that isolate Q shared a 100% similarity with Stenotrophomonas maltophilia.

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