Hepatoprotective effect of Ocimum basilicum aqueous extract against doxorubicin-induced hepatic damage in albino rats
##plugins.themes.bootstrap3.article.sidebar##
Issue
Section
Articles
##plugins.themes.bootstrap3.article.main##
Abstract
Abstract. Boulaares I, Derouiche S, Guemari IY. 2024. Hepatoprotective effect of Ocimum basilicum aqueous extract against doxorubicin-induced hepatic damage in albino rats. Asian J Trop Biotechnol 21: 68-74. This investigation aimed to evaluate the effect of Ocimum basilicum L. aqueous extract (ObE) on hepatotoxicity induced by doxorubicin in rats. Standard procedures were used to extract bioactive compounds and to analyze phytochemical compounds quantitatively and qualitatively. The in-vivo study was performed using 15 female albino rats that were grouped into 3 groups (n=5): a control group, a doxorubicin-treated group (DOX), and a group co-treated with doxorubicin and O. basilicum aqueous extract (DOX + ObE). Various biochemical, enzymatic, and liver oxidative stress markers were analyzed. Phytochemical results demonstrated that the ObE contained highly phenolic compounds. The results of the in vivo study showed that the treatment of doxorubicin caused a significant decrease of WBC (P?0.01), lymphocyte (P?0.05), also a significant increase in GOT (p<0.05), CPK (p<0.001) and LDH (p<0.05) activities compared to control group/normal rats. In addition, doxorubicin-treated rats resulted in a significant decrease in GSH (p<0.01) and an increase in MDA (p<0.01), SOD (p<0.001), and GST (p<0.01) levels in liver cells compared to the control group. Treatment with ObE partially reversed all of the previously mentioned parameters. This study indicated that basil aqueous extract's antioxidant activity could protect the liver from the harmful effects of doxorubicin or the destructive effects of various liver diseases such as hepatitis or other drug hepatotoxicity.
##plugins.themes.bootstrap3.article.details##
References
Abboud SH, Hadi NR, Assad HC, Shaker RA. 2018. Cardioprotective effects of erythropoietin against doxorubicin-induced cardiotoxicity in rats. World Heart J 9 (3): 212-230.
Acila S, Derouiche S, Allioui N. 2024. Embryo growth alteration and oxidative stress responses in germinating Cucurbita pepo seeds exposed to cadmium and copper toxicity. Sci Rep 14: 8608. DOI: 10.1038/s41598-024-58635-1.
Ahn MR, Kumazawa S, Hamasaka T, Bang KS, Nakayama T. 2004. Antioxidant activity and constituents of propolis collected in various areas of Korea. J Agric Food Chem 52 (24): 7286-7292. DOI: 10.1021/jf048726s.
Ajzashokouhi AH, Bostan HB, Jomezadeh V, Hayes AW, Karimi G. 2019. A review on the cardioprotective mechanisms of metformin against doxorubicin. Human Exp Toxicol 39 (3): 237-248. DOI: 10.1177/0960327119888277.
Allameh A, Niayesh-Mehr R, Aliarab A, Sebastiani G, Pantopoulos K. 2023. Oxidative stress in liver pathophysiology and disease. Antioxidants 12 (9): 1653. DOI: 10.3390/antiox12091653.
AlQahtani AA, Osman A-MM, Damanhouri ZA, Al-Kreathy HM, Al-Malky HS, Ramadan WS, Alharthi SE, Kamel FO. 2019. Cardioprotective effect of marine astaxanthin on doxorubicin-induced cardiotoxicity in normal rats. J Pharm Res Intl 27 (3): 1-11. DOI: 10.9734/jpri/2019/v27i330170.
Anber ZNH. 2018. Effect of doxorubicin and cyclophosphamide regimen versus taxane on liver enzymes in Iraqi women with breast cancer. Biomed Res 29 (21): 3869-3873. DOI: 10.4066/biomedicalresearch.29-18-1140.
Antonucci S, Di Sante M, Tonolo F. 2021. The determining role of mitochondrial reactive oxygen species generation and monoamine oxidase activity in doxorubicin-induced cardiotoxicity. Antioxid Redox Signal 34 (7): 531-550. DOI: 10.1089/ars.2019.7929.
Aruna P, Gayathiri NM. 2018. Cardioprotective activity of telmisartan, metformin and its combination against doxorubicin induced myocardial infarction in rat model. Res J Pharm Technol 11 (12): 5293-5296. DOI: 10.5958/0974-360X.2018.00964.2.
Beauchamp C, Fridovich I. 1971. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44 (1): 276-287. DOI: 10.1016/0003-2697(71)90370-8.
Belhouala K, Benarba B. 2021. Medicinal plants used by traditional healers in Algeria: A multiregional ethnobotanical study. Front Pharmacol 12: 760492. DOI: 10.3389/fphar.2021.760492.
Boizot N, Charpentier JP. 2006. Rapid method for evaluating the content of phenolic compounds in the organs of a forest tree. The Cahier des Techniques of INRA, Paris.
Boulaares I, Derouiche S, Guemari IY 2024a. Impact of doxorubicin chemotherapy on oxidative stress status in heart and liver: An experimental study on rats. Pharm Sci Anal Res J 6 (3): 180067.
Boulaares I, Derouiche S, Guemari IY. 2024b. Protective effect of ObE against doxorubicin-induced immunosuppression and cardiotoxicity in rats. Res J Pharm Technol 17 (4): 1839-1843. DOI: 10.52711/0974-360X.2024.00292.
Boulaares I, Derouiche S, Niemann J. 2024c. HPLC-Q-TOF-MS analysis of phenolic compounds, in vitro biological activities and in vivo acute toxicity evaluation of Ocimum basilicum L. Fresenius Environ Bull 33 (2): 73-82.
Boulaares I, Derouiche S, Niemann J. 2024d. Ocimum basilicum L.: A systematic review on pharmacological actions and molecular docking studies for anticancer properties. J Biochem Technol 15 (1): 12-18. DOI: 10.51847/TlkGjN14PM.
Boulaares I, Guemari IY, Derouiche S. 2020. Analysis of some hematological and biochemical markers in women cancer patients receiving doxorubicin chemotherapy. J Pharm Biosci 8: 29-34. DOI: 10.20510/UKJPB/8/I1/1581327899.
Chetehouna S, Derouiche S, Atoussi O. 2024a. Identification of new alkaloids from algerian purslane by HPLC-QTOF-MS and beneficial effect of purslane enriched with zinc on experimental alzheimer disease in rats. Curr Trends Biotechnol Pharm 18 (1): 1595-1607. DOI: 10.5530/ctbp.2024.1.8.
Chetehouna S, Derouiche S, Réggami Y, Boulaares I, Frahtia A. 2024b. Gas chromatography analysis, mineral contents and anti-inflammatory activity of Sonchus maritimus. Trop J Nat Prod Res 8 (4): 6787-6798. DOI: 10.26538/tjnpr/v8i4.7.
Choi WG, Kim DK, Shin Y, Park R, Cho YY, Lee JY, Kang HC, Lee HS. 2020. Liquid chromatography-tandem mass spectrometry for the simultaneous determination of doxorubicin and its metabolites doxorubicinol, doxorubicinone, doxorubicinolone, and 7-deoxydoxorubicinone in mouse plasma. Molecules 25 (5): 1254. DOI: 10.3390/molecules25051254.
Derouiche S, Chetehouna S, Atoussi W. 2022. The effects of aqueous leaf extract of Portulaca oleracea on haemato-biochemical and histopathological changes induced by sub-chronic aluminium toxicity in male wistar rats. Pharm Res-Mod Chin Med 4: 100101. DOI: 10.1016/j.prmcm.2022.100101.
Derouiche S, Guemari IY, Boulaares I. 2020. Characterization and acute toxicity evaluation of the MgO nanoparticles synthesized from aqueous leaf extract of Ocimum basilicum L. Alger J Biosci 1 (1): 1-6. DOI: 10.5281/zenodo.4041021.
Derouiche S. 2020. Current review on herbal pharmaceutical improve immune responses against COVID-19 infection. Res J Pharm Dos Forms Technol 12 (3): 181-184. DOI: 10.5958/0975-4377.2020.00031.2.
Ferreira TS, Moreira CZ, Cária NZ, Victoriano G, Silva WF, Magalhães JC. 2014. Phytotherapy: an introduction to its history, use and application. Rev Bras Plant Med 16 (2): 290-298. DOI: 10.1590/S1516-05722014000200019.
Gasser A, Chen YW, Audebrand A, Daglayan A, Charavin M, Escoubet B, Karpov P, Tetko I, Chan MWY, Cardinale D, Désaubry L, Nebigil CG. 2019. Prokineticin Receptor-1 signaling inhibits dose- and time-dependent anthracycline-induced cardiovascular toxicity via myocardial and vascular protection. JACC CardioOncol 1 (1): 84-102. DOI: 10.1016/j.jaccao.2019.06.003.
Golemis EA, Scheet P, Beck TN. 2018. Molecular mechanisms of the preventable causes of cancer in the United States. Genes Dev 32 (13-14): 868-902. DOI: 10.1101/gad.314849.118.
Guemari IY, Boulaares I, Derouiche S. 2024. Study of the role of oxidative stress in pathophysiology of cardiovascular diseases. Collect J Cardiovasc Med 1 (1): ART0014. DOI: 10.70107/collectjcardiovasc-ART0014.
Guo RM, Xu WM, Lin JC, Mo LQ, Hua XX 2013. Activation of the p38 MAPK/NF-?B pathway contributes to doxorubicin-induced inflammation and cytotoxicity in H9c2 cardiac cells. Mol Med Rep 8 (2): 603-608. DOI: 10.3892/mmr.2013.1554.
Habig WH, Pabst MJ, Jakoby WB. 1974. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem 249 (22): 7130-7139. DOI: 10.1016/S0021-9258(19)42083-8.
Hajare AA, Powar Trupti A, Bhatia Neela M, More Harinath N. 2016. Development and validation of RP-HPLC method for determination of doxorubicin hydrochloride from vacuum foam dried formulation. Res J Pharm Techol 9 (9): 1352-1356. DOI: 10.5958/0974-360X.2016.00259.6.
Hasnat H, Shompa SA, Islam MM et al. 2024. Flavonoids: A treasure house of prospective pharmacological potentials. Heliyon 10 (6): e27533. DOI: 10.1016/j.heliyon.2024.e27533.
Hozayen WG, Seif HSA. 2011. Protective effects of rutin and hesperidin against doxorubicin-induced lipodystrophy and cardiotoxicity in albino rats. Am J Sci 7 (12): 765-775.
Ibrahim YS, Akram A, Rajab WJ, Ahmed MMA, Mahmood MA. 2022. Assessment of some biochemical parameters among pleural liquid and serum during platinum-based chemotherapy against ovarian cancer. Res J Pharm Technol 15 (12): 5665-1. DOI: 10.52711/0974-360X.2022.00955.
Lei J, Wang H, Zhu D, Wan Y, Yin L. 2020. Combined effects of avasimibe immunotherapy, doxorubicin chemotherapy, and metal-organic frameworks nanoparticles on breast cancer. J Cell Physiol 235 (5): 4814-4823. DOI: 10.1002/jcp.29358.
Lódi M, Priksz D, Fülöp GÁ et al. 2019. Advantages of prophylactic versus conventionally scheduled heart failure therapy in an experimental model of doxorubicin-induced cardiomyopathy. J Transl Med 17: 1-16. DOI: 10.1186/s12967-019-1978-0.
Ma Y, Yang L, Ma J, Lu L, Wang X, Ren J, Yang J. 2017. Rutin attenuates doxorubicin-induced cardiotoxicity via regulating autophagy and apoptosis. Biochim Biophys Acta Mol Basis Dis 1863 (8): 1904-1911. DOI: 10.1016/j.bbadis.2016.12.021.
Pistelli L, Ascrizzi R, Giuliani C, Cervelli C, Ruffoni B, Princi E, Pistelli L. 2020. Growing basil in the underwater biospheres of Nemo's Garden®: Phytochemical, physiological and micromorphological analyses. Sci Hortic 259: 108851. DOI: 10.1016/j.scienta.2019.108851.
Rahman MM, Rahaman MS, Islam MR et al. 2021. Role of phenolic compounds in human disease: Current knowledge and future prospects. Molecules 27 (1): 233. DOI:10.3390/molecules27010233.
Sadzuka Y, Sugiyama T, Shimoi K, Kinae N, Hirota S. 1997. Protective effect of flavonoids on doxorubicin-induced cardiotoxicity. Toxicol Lett 92: 1-7. DOI: 10.1016/s0378-4274(97)00028-3.
Salmerón-Manzano E, Garrido-Cardenas JA, Manzano-Agugliaro F. 2020. Worldwide Research Trends on Medicinal Plants. Intl J Environ Res Public Health 17 (10): 3376. doi:10.3390/ijerph17103376.
Sastre J, Pallardó FV, García de la Asunción J, Viña J. 2000. Mitochondria, oxidative stress and aging. Free Radic Res 32 (3): 189-198. DOI: 10.1080/10715760000300201.
Shaty MH. Al-Ezzi MI. Arif IS, Basil D. 2019. Effect of metformin on inflammatory markers involved in cardiotoxicity induced by doxorubicin. Res J Pharm Technol 12: 5815-5821. DOI: 10.5958/0974-360X.2019.01007.2.
Shosha MI, El-Ablack FZ, Saad EA. 2023. Glycine protects against doxorubicin-induced heart toxicity in mice. Amino Acids 55 (5): 679-693. DOI: 10.1007/s00726-023-03261-w.
Siegel RL, Miller KD, Jemal A. 2020. Cancer statistics, 2020. CA Cancer J Clin 70 (1): 7-30. DOI: 10.3322/caac.21590.
Syahputra RA, Harahap U, Dalimunthe A, Nasution MP, Satria D. 2022. The role of flavonoids as a cardioprotective strategy against doxorubicin-induced cardiotoxicity: A review. Molecules 27 (4): 1320. DOI: 10.3390/molecules27041320.
Tariq J, Shumaila S, Riaz S, Afzal S, Hussain S, Riaz S, Uzair M, Chaudhary BA. 2016. Phytochemical and biological evaluation of Ocimum basilicum roots. Intl J Pharm Pharm Res Human 2: 292-301.
Weckbecker G, Cory JG. 1988. Ribonucleotide reductase activity and growth of glutathione-depleted mouse leukemia L1210 cells in vitro. Cancer Lett 40 (3): 257-264. DOI: 10.1016/0304-3835(88)90084-5.
Zhakipbekov K, Turgumbayeva A, Akhelova S. 2024. Antimicrobial and other pharmacological properties of Ocimum basilicum, Lamiaceae. Molecules 29 (2): 388. DOI: 10.3390/molecules29020388.
Zhao J, Wang P, Pan Y. 2022. Predicting liver disorder based on machine learning models. J Eng 2022: 978-984. DOI: 10.1049/tje2.12184.