Diversity, abundance, and enzyme activity of Actinomycetes recovered from tropical freshwater sediment for potential biotechnological applications
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Abstract. Ajuzieogu CA, Onu GO, Onyekachi EO. 2025. Diversity, abundance, and enzyme activity of Actinomycetes recovered from tropical freshwater sediment for potential biotechnological applications. Asian J Trop Biotechnol 22: 23-29. Actinomycetes are a set of Gram-positive bacteria known for their ability to produce a wide variety of secondary metabolites, including antibiotics and enzymes. This study assessed the diversity of Actinomycetes in tropical freshwater sediment and their amylase production potential. Sediment samples were obtained from four points (shore, middle, downstream, and upstream) within a tropical freshwater system. Physicochemical analysis, bacterial isolation, and enumeration were conducted following standard methods. Tentative identification of isolates was done using culture-dependent methods. Results showed that the sediment samples belonged to the loam soil textural class; upstream sediment recorded the highest pH and total organic carbon content (6.3 and 0.98%, respectively), and middle sediment recorded the highest nitrate content (9.632 mg/kg). In comparison, sediment from the shore recorded the lowest pH (5.70) and highest phosphate level (8.716 mg/kg). The highest total culturable heterotrophic counts were observed in sediment from the middle point. A total of 9 Actinomycetes species were recovered, out of which six (6) were positive for amylase activity. The isolates were tentatively identified as Micrococcus spp., Streptomyces sp., Streptomyces coelicolor, and Actinomyces viscosus. This study revealed that tropical freshwater sediments harbor a diverse and abundant bacterial community, particularly Actinomycetes, with possible potential for biotechnological exploration.
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Adeyemo OM, Ja’afaru MI, Adams FV. 2021. Isolation, characterization, antimicrobial and other bioactivity profiles of three Streptomyces strains isolated from Lake Gerio, Yola, Adamawa State, Nigeria. Bull Natl Res Cent. 45: 1-13.
Ahmad I, Althubiani AS, Dar MS, Samreen Qais FA, Abulreesh HH, Alshehrei F. 2019. Actinomycetes as continued source of new antibacterial leads. Antibacterial Drug Discovery to Combat MDR: Natural Compounds, Nanotechnology and Novel Synthetic Sources, 327-349.
Alam K, Mazumder A, Sikdar S, Zhao YM, Hao J, Song C, Li A. 2022. Streptomyces: The biofactory of secondary metabolites. Front Microbiol. 13: 968053.
American Public Health Association (APHA) (2017). Standard methods for the examination of water and wastewater (23rd Edition). Washington DC.
Ataikiru TL, Okerentugba PO, Okpokwasili GC. 2020. Identification of carbofuran and paraquat degrading microorganisms from soil. South Asian J Res Microbiol. 7: 40-52.
Cheesbrough M. 2010. District laboratory practice in tropical countries. 2nd Edition, Cambridge University Press, Cambridge, United Kingdom.
Das S, Lyla PS, Khan SA. 2019. Exploration of Actinomycetes from Freshwater Sediments: A Potential Source for Novel Bioactive Compounds. Front Microbiol. 10: 338.
De Simeis D, Serra S. 2021. Actinomycetes: A never-ending source of bioactive compounds—An overview on antibiotics production. Antibiotics. 10(5): 483.
Francioli D, Schulz E, Lentendu G, Wubet T, Buscot F, Reitz T. 2016. Mineral vs. organic amendments: microbial community structure, activity and abundance of agriculturally relevant microbes are driven by long-term fertilization strategies. Front Microbiol. 7: 1446.
Kalisz HM. 2018. Microbial proteinases. Adv Biochem Eng/Biotech. 361-365.
Kontro MH, Yaradoddi JS, Banapurmath NR, Ganachari SV, Hungund BS. 2022. Biotechnological Importance of Actinomycetes. In: Yaradoddi JS, Kontro MH, Ganachari SV (eds). Actinobacteria: Ecology, Diversity, Classification and Extensive Applications. Springer Nature, Singapore.
Kumar PP, Raj JPP, Christhudas IVSN, Jansi RS, Murugan N, Agastian, P, Arunachalam C, Alharbi SA. 2015. Screening of Actinomycetes for enzyme and antimicrobial activities from the soil sediments of Northern Tamil Nadu, South India. J Biol Act Prod Nat. 1-13.
Lalas S, Athanasiadis V, Dourtoglou VG. 2018. Humic and fulvic acids as potentially toxic metal reducing agents in water. CLEAN–Soil, Air, Water. 46(2): 1700608.
Law JWF, Pusparajah P, Ab Mutalib NS, Wong SH, Goh BH, Lee LH. 2019. A review on mangrove actinobacterial diversity: the roles of Streptomyces and novel species discovery. Progress In Microbes & Molecular Biology. 2(1).
Li W, Xie L, Zhao C, Hu X, Yin C. 2023. Nitrogen fertilization increases soil microbial biomass and alters microbial composition especially under low soil water availability. Microbial Ecol. 86(1): 536-548.
Maron PA, Sarr A, Kaisermann A, Lévêque J, Mathieu O, Guigue J, Ranjard L. 2018. High microbial diversity promotes soil ecosystem functioning. Appl Environ Microbiol. 84(9): e02738-17.
Olanrewaju OS, Babalola OO, Mononyane M, Serepa-Dlamini MH. 2020. Actinomycetes in Freshwater Sediments: Diversity and Biotechnological Potentials. Rev Environ Sci Biotech. 19(4): 605-624.
Patin NV, Schorn M, Aguinaldo K, Lincecum T, Moore BS, Jensen PR. 2017. Effects of Actinomycetes secondary metabolites on sediment microbial communities. Appl Environ Microbiol. 83(4): e02676-16.
Ribeiro I, Antunes JT, Alexandrino DA, Tomasino MP, Almeida E, Hilário A, Carvalho MF. 2023. Actinobacteria from Arctic and Atlantic deep-sea sediments—Biodiversity and bioactive potential. Front Microbiol. 14: 1158441.
Sarkar G, Suthindhiran K. 2022. Diversity and biotechnological potential of marine Actinomycetes from India. Indian J Microbiol. 62(4): 475-493.
Selim MSM, Abdelhamid SA, Mohamed SS. 2021. Secondary metabolites and biodiversity of Actinomycetes. J Gen Eng Biotech. 19(1): 72.
Shigeri Y, Matsui T, Watanabe K. 2019. Decomposition of intact chicken feathers by a thermophile in combination with an acidulocomposting garbage treatment process. Biosci Biotech Biochem. 73(11): 2519-2521.
Tonkova A. 2016. Microbial starch converting enzymes of the amylase family. In: Ray CR, Words OP. (eds). Microbial Biotechnology in Horticulture. 1: 421-472.
Xia Q, Rufty T, Shi W. 2020. Soil microbial diversity and composition: Links to soil texture and associated properties. Soil Biol Biochem. 149: 107953.
Yang F, Zhang S, Cheng K, Antonietti M. 2019. A hydrothermal process to turn waste biomass into artificial fulvic and humic acids for soil remediation. Sci Total Environ. 686: 1140-1151.
Zanane C, Latrache H, Elfazazi K, Zahir H, Ellouali M. 2018. Isolation of Actinomycetes from different soils of Beni Amir Morocco. J Mat Environ Sci. 9(10): 2994-3000.