Hidayati Z, Arofah AN, Fakhira J. 2018. Cattle urine as a low-cost medium for accelerate growth, biomass productivity, and lipid production of Botryococcus braunii: future energy. Bioteknologi 15: 47-50. The increasing necessities of global energy can trigger an energy crisis. Microalgae is a unicellular microorganism which has potency as bioenergy such as biofuel and efficient alternative to substitute fossil resources. Botryococcus braunii is one of the microalgae that has high lipid concentration around 25-80%, this characteristic shows its potency to produce biofuel. This research takes into 15 days which purpose to utilize cattle urine as an alternative medium for growth B. braunii. Some of components in cattle urine are 95% water, urea, 2.5% mineral salt, hormone, enzyme, 69% nitrogen, sulfur, and magnesium. The components of cattle urine can substitute macronutrient and micronutrient in others synthesis medium. This research was designed by using Random Design Complete (RAL) with seven variables: control culture B. braunii (medium macronutrient+ micronutrient Jhonson); cultures B. braunii with 5%, 7.5%, 10%, 12.5%, 15%, and 17.5% concentration of cattle urine+micronutrient Jhonson. The method of this research are divided into three steps; preparation, testing, and analysis of biomass also lipid extraction. The data from the observations of biomass were analyzed with linear regression equation. All of data was analyzed by Ms. Excel program. The result from this research is showed in culture B. braunii with 12.5% of concentration cattle urine that can accelerate the growth of cell B. braunii being maximal which production 1.425 mg/L biomass in days 12 and also B. braunii culture with 12.5% of concentration cattle urine can accelerate biomass productivity of B.brauni which production 9.018 g/L biomass, whereas with added cattle urine as medium is not given significantly to lipid production.