Short Communication: Evaluation of antimicrobial activity of hydroxyapatites from Anadara granosa and Achatina fulica against Porphyromonas gingivalis

Abstract

Abstract. Anitasari S, Yuniati Y, Agustin S. 2019. Short Communication: Evaluation of antimicrobial activity of Anadara granosa and Achatina fulica hydroxyapatites against Porphyromonas gingivalis. Bioteknologi 16: 1-4. Hydroxyapatite bioceramic (HAp) plays a key role in reconstructive surgeries in both medicine and dentistry.  However, its use is often saddled with the occurrence of post-surgery infection, thereby creating the need for antimicrobial therapy. The antibiotics used should support the body’s defense mechanisms. Applying antimicrobials locally after surgery is promising, but the concentration must be lethal to pathogens and must be safe for normal human flora, as most oral antibiotic therapies are not effective. This study aimed to create HAp with good antimicrobial activity without causing harm to normal human flora. Antimicrobial activity of various combinations of hydroxyapatites from A. granosa and A. fulica against P. gingivalis were determined by the Kirby-Bauer method. The results showed that the inhibitory zone of HAp A. granosa (8.3±0) was similar to positive control Chlorhexidine (8.5± 0.1), but no activity was found in HAp of A. fulica. The HAp combination of A. granosa and A. fulica, at the ratio of 6: 4, had the smallest inhibitory zone (3.5±0 mm). It can be concluded that the differences in antibacterial activity of HAp from A. granosa’s and A. fulica’s might be influenced by concentration and mineral of HAp. Based on the results, it can be concluded that the inhibitory concentration was obtained at the ratio of 6: 4.