Short Communication: Analysis of purity and concentration of DNA extracted from intron patho gene-spin extraction on crab processed food product samples

Abstract

Abstract. Sophian A, Purwaningsih R, Muindar, Igirisa EPJ, Amirullah ML. 2021. Short Communication: Analysis of purity and concentration of DNA extracted from intron patho gene-spin extraction on processed crab food product samples. Asian J Trop Biotechnol 18: 28-31. The purpose of this study was to optimize the Intron patho gene-spin DNA/RNA extraction kit on processed crab food products so that it can be used for testing samples, other than viral and bacterial samples. The method used in purity and concentration analysis to analyze total DNA (yield) is the absorbance method using a nanophotometer. The method used to isolate the sample DNA is the centrifuge column or spin column method. The research data showed that the extracted sample concentration was in the range of 15.15-15.75, with an average of 15.46. While the purity values measured at the A260/A280 wavelengths were obtained with a purity range between 2,124-2,346. The total DNA analysis (yield) results obtained an average of 755.04, with the lowest yield value of 575.50 and the highest yield value of 787.50. As a suggestion for further research, it is hoped that modification can be made to perfect the isolated DNA so that it has better purity and concentration values.