Cryopreservation of Gaga’ chicken semen from South Sulawesi, Indonesia with the addition of L-carnitine, hyaluronic acid, sucrose and their combination in diluent

##plugins.themes.bootstrap3.article.main##

KHAERUDDIN
https://orcid.org/0000-0001-5935-4602
SRI WAHJUNINGSIH
GATOT CIPTADI
MUHAMMAD YUSUF
HERMAWANSYAH
SAHIRUDDIN

Abstract

Abstract. Khaeruddin, Wahjuningsih S, Ciptadi G, Yusuf M, Hermawansyah, Sahiruddin. 2022. Cryopreservation of Gaga’ chicken semen from South Sulawesi, Indonesia with the addition of L-carnitine, hyaluronic acid, sucrose and their combination in diluent. Biodiversitas 23: 3297-3302. Gaga' chicken (Gallus gallus domesticus) is Indonesian germplasm that needs to be preserved with cryopreservation technology. Therefore, this study aims to determine the effect of adding L-Carnitine, hyaluronic acid, and sucrose as well as their combination in a diluent on the quality of Gaga' chicken spermatozoa after freeze-thawing. A completely randomized design was used along with 6 treatments, namely P0, P1, P2, P3, P4, and P5, containing Ringer acetate-egg yolk (RAEY) diluent (P0), RAEY + 0.063 mM hyaluronic acid (P1), RAEY + 1 mM L-carnitine (P2), RAEY + 1 mM sucrose (P3), RAEY + 0.063 mM hyaluronic acid + 1 mM L-carnitine (P4), and RAEY + 0.063 mM hyaluronic acid + 1 mM L-carnitine + 1mM sucrose (P5). Liquid semen was packed in a 0.25 mL straw, equilibrated at 5oC for 2 hours, and placed 3 cm above the surface of liquid nitrogen. It was then immersed in nitrogen for 24 hours and thawed at 60°C for 5 seconds. The results showed that there was a significant difference (P<0.05) in the motility, viability, and kinematic variables of spermatozoa after freeze-thawing. P1, P2, P3, and P4 increased progressive motility and curvilinear velocity, while viability increased with P1, P2, and P4 treatments. Furthermore, the addition of Hyaluronic acid, L-carnitine, or their combination in the RAEY diluent can improve the sperm quality of Gaga' chickens.

##plugins.themes.bootstrap3.article.details##

References
Banihani S, Agarwal A, Sharma R, Bayachou, M. 2014. Cryoprotective effect of L-carnitine on motility, vitality and DNA oxidation of human spermatozoa. Andrologia 46: 637–641. DOI: 10.1111/and.12130.
Braga PC, Dal Sasso M, Lattuada N, Greco V, Sibilia V, Zucca E, Stucchi L, Ferro E, Ferrucci F. 2015. Antioxidant activity of hyaluronic acid investigated by means of chemiluminescence of equine neutrophil bursts and electron paramagnetic resonance spectroscopy. J Vet Pharmaco Therap 38: 48–54. DOI: 10.1111/jvp.12141.
Bugiwati SRA, Ashari, F. 2013. Crowing sound analysis of Gaga’ chicken: Local chicken from South Sulawesi Indonesia. Int J Plant Anim Environ Sci 3(2): 162–168.
Burrows WH, Quinn JP. 1937. The collection of spermatozoa from the domestic fowl and turkey. Poult Sci 16(1): 19–24. DOI: 10.3382/ps.0160019.
Cerolini S, Zainiboni L, Maldjian A, Gliozzi T. 2006. Effect of docosahexaenoicacid and ?-tocopherol enrichment in chicken sperm on semen quality, sperm lipid composition and susceptibility to peroxidation. Theriogenology 66: 877–886. DOI: 10.1016/j.theriogenology.2006.02.022.
Chen S, Ren J, Chen, R. 2019. Cryopreservation and Desiccation Preservation of Cells. In M. Moo-Young (Ed.), Comprehensive Biotechnology (3rd ed., pp. 157–166). Oxford: Pergamon. DOI: 10.1016/B978-0-444-64046-8.00451-1.
Colagar AH, Karimi F, Jorsaraei SGA. 2013. Correlation of sperm parameters with semen lipid peroxidation and total antioxidants levels in astheno- and oligoasheno- teratospermic men. Iran Red Crescent Med J 15(9): 780–785. DOI: 10.5812/ircmj.6409.
de Souza CV, Brandão FZ, Santos JDR, Alfradique VAP, Santos VMBD, Morais MCC, Rangel PSC, da Silva AA, Souza-Fabjan JMG. 2019. Effect of different concentrations of l-carnitine in extender for semen cryopreservation in sheep. Cryobiology 89: 104–108. DOI: 10.1016/j.cryobiol.2019.05.009.
Farooq U, Malecki IA, Mahmood M, Martin GB. 2017. Appraisal and standardization of curvilinear velocity (VCL) cut-off values for CASA analysis of Japanese quail ( Coturnix japonica ) sperm. Reprod Domest Anim 52(3): 389–396. DOI: 10.1111/rda.12920.
Fattah A, Sharafi M, Masoudi R, Shahverdi A, Esmaeili V, Najafi A. 2016. L-Carnitine in rooster semen cryopreservation: Flow cytometric, biochemical and motion findings for frozen-thawed sperm. Cryobiology 74: 148–153. DOI: 10.1016/j.cryobiol.2016.10.009.
Ke C, Sun L, Qiao D, Wang D, Zeng X. 2011. Antioxidant acitivity of low molecular weight hyaluronic acid. Food Chem Toxicol 49(10): 2670–2675. DOI: 10.1016/j.fct.2011.07.020.
Kumar KP, Swathi B, Shanmugam M. 2019. Effect of L-glycine and L-carnitine on post-thaw semen parameters and fertility in chicken. Slovak J Anim Sci 25(1): 1–8.
Lisboa FL, Hartwig FP, Maziero RRD, Monteiro GA, Papa FO, Dell’aqua JrJA. 2012. Use of L-carnitine and acetyl-L-carnitine in cooled-stored stallion semen. J Equine Vet Sci 32(8): 493-494. DOI: 10.1016/j.jevs.2012.06.057.
Lotfi S, Mehri M, Sharafi M, Masoudi R. 2017. Hyaluronic acid improves frozen-thawed sperm quality and fertility potential in rooster. Anim Reprod Sci 184: 204–210. DOI: 10.1016/j.anireprosci.2017.07.018.
Madeddu M, Mosca F, Sayed AA, Zaniboni L, Mangiagalli MG, Colombo E, Cerolini S. 2016. Effect of cooling rate on the survival of cryopreserved rooster sperm: Comparison of different distances in the vapor above the surface of the liquid nitrogen. Anim Reprod Sci 171: 58–64. DOI: 10.1016/j.anireprosci.2016.05.014.
Manee-in S, Parmornsupornvichit S, Kraiprayoon S, Tharasanit T, Chanapiwat P, Kaeoket K. 2014. L-carnitine supplemented extender improves cryopreserved-thawed cat epididymal sperm motility. Asian Australas J Anim Sci 27(6): 791–796. DOI: 10.5713/ajas.2013.13565.
Mongioi L, Calogero A, Vicari E, Condorelli R, Russo G, Privitera S, Morgia G, La Vignera S. 2016. The role of carnitine in male infertility. Andrology 4: 800–807. DOI: 10.1111/andr.12191.
Mosca F, Madeddu M, Sayed AA, Zaniboni L, Iaffaldano N, Cerolini S. 2016. Combined effect of permeant and non-permeant cryoprotectants on the quality of frozen/thawed chicken sperm. Cryobiology 73(3): 343–347. DOI: 10.1016/j.cryobiol.2016.10.001.
Mussa NJ, Ratchamak R, Ratsiri T, Vongpralub T, Boonkum W, Semaming Y, Chankitisakul V. 2021. Lipid profile of sperm cells in Thai native and commercial roosters and its impact on cryopreserved semen quality. Trop Anim Health Prod, 53(2): 321. DOI: 10.1007/s11250-021-02664-9.
Partyka A, ?ukaszewicz E, Ni?a?skia W. 2012. Effect of cryopreservation on sperm parameters, lipid peroxidation and antioxidant enzymes activity in fowl semen. Theriogenology 77: 1497–1504. DOI: 10.1016/j.theriogenology.2011.11.006.
Partyka A, Rodak O, Bajzert J, Kochan J, NihaNski W. 2017. The effect of L-Carnitine, hypotaurine, and taurine supplementation on the quality of cryopreserved chicken semen. BioMed Res Int 7279341. DOI: 10.1155/2017/7279341.
Qian L, Yu S, Zhou Y. 2016. Protective effect of hyaluronic acid on cryopreserved boar sperm. Int J Biol Macromol 87: 287–289. DOI: 10.1016/j.ijbiomac.2016.02.075.
Salih SA, Daghigh-Kia H, Mehdipour M, Najaf A. 2021. Does ergothioneine and thawing temperatures improve rooster semen postthawed quality?. Poult Sci 100(10): 101405. DOI: 10.1016/j.psj.2021.101405.
Sangani AK, Masoudi AA, Torshizi RV. 2017. Association of mitochondrial function and sperm progressivity in slow- and fast-growing roosters. Poult Sci 96: 211–219. DOI: 10.3382/ps/pew273.
Santiago-Moreno J, Castano C, Toledano-Diaz A, Coloma MA, Lopez-Sebastian A, Prieto MT, Campo JL. 2011. Semen cryopreservation for the creation of a Spanish poultry breeds cryobank: Optimization of freezing rate and equilibration time. Poult Sci 90: 2047–2053. DOI: 10.3382/ps.2011-01355.
Sayed MAM, Abouelezz FMK, Abdel-Wahab AAM. 2017. Analysis of sperm motility, velocity and morphometry of three Egyptian Indigenous chicken strains. Egypt Poult Sci J 37(4): 1173–1185. DOI: 10.21608/epsj.2017.5605.
Singh A, Li P, Beachley V, McDonnell P, Elisseeff JH. 2015. A hyaluronic acid-binding contact lens with enhanced water retention. Cont Lens Anterior Eye 38(2): 79–84. DOI: 10.1016/j.clae.2014.09.002.
Sloter E, Schmid TE, Marchetti F, Eskenazi B, Nath J, Wyrobek AJ. 2006. Quantitative effects of male age on sperm motion. Hum Reprod 21(11): 2868–2875. DOI: 10.1093/humrep/del250.
Surai PF. 2015a. Antioxidant action of carnitine: molecular mechanisms and practical applications. EC Veterinary Science 2: 66–84.
Surai PF. 2015b. Carnitine enigma: from antioxidant action to vitagene regulation. Part 2. Transcription factors and practical applications. J Vet Sci Med 3(2): 14. DOI: 10.13188/2325-4645.1000018.
Thananurak P, Chuaychu-noo N, Thélie A, Phasuk Y, Vongpralub T, Blesbois E. 2018. Sucrose increases the quality and fertilizing ability of cryopreserved chicken sperms in contrast to raffinose. Poult Sci 98: 4161-4171. DOI: 10.3382/ps/pez196.
Thélie A, Bailliard A, Seigneurin F, Zerjal T, Tixier-Boichard M, Blesbois E. 2019. Chicken semen cryopreservation and use for the restoration of rare genetic resources. Poult Sci 98(1): 447–455. DOI: 10.3382/ps/pey360.
Williams JK, Yoo JJ, Atala A. 2019. Regenerative medicine approaches for tissue engineered heart valves. In Atala A, Lanza R, Mikos AG, Nerem R (Eds.). Principles of Regenerative Medicine (3rd ed., pp. 1041–1058). US: Academic Press. DOI: 10.1016/B978-0-12-809880-6.00059-X.
Zhang JJ, Do HL, Chandimali N, Lee SB. 2018. Non-thermal plasma treatment improves chicken sperm motility via the regulation of demethylation levels. Sci Rep 8: 7576. DOI: 10.1038/s41598-018-26049-5.