Effectiveness of biofungicide formula on rhizome rot disease of red ginger and its plant growth




Abstract. Marwan H, Hayati I, Mulyati S. 2023. Effectiveness of biofungicide formula on rhizome rot disease of red ginger and its plant growth. Biodiversitas 24: 2143-2148. Rhizome rot disease is a serious problem in red ginger cultivation in Indonesia. The development of organic red ginger cultivation to meet the demand for phytopharmaca raw materials requires a method of controlling rhizome rot disease without synthetic pesticides. The application of microbial biopesticides is one of the disease control methods that can be used to control rhizome rot disease in red ginger plants caused by Fusarium oxysporum. The purpose of this study was to determine the effect of biofungicide formula containing Trichoderma sp. TBP1 and Bacillus spp. (PBC25 and PBC32 isolates) on plant growth and disease incidence in red ginger. The results of in vitro testing showed that Trichoderma sp. (TBP1) isolate was able to inhibit the growth of F. oxysporum by 83%. The inhibition of F. oxysporum by Bacillus spp. isolates, namely PBC25 and PBC32 were 53.3% and 50%. It was observed that application of several biofungicide formulas containing Trichoderma sp. and Bacillus spp. on ginger rhizome before planting and 30 days after planting enhanced the growth of ginger plants and suppressed the development of rhizome rot disease between 78.57 - 93.3%.


Acharya B, Regmi H, Ngangbam AK, Nongmaithem BD. 2016. Management of rhizome rot disease of ginger using eco-friendly natural products. Indian J Agri Res 50(6):599–603.
Ahmad AGM, Abo-Zaid GA, Matar SM, Hafez EE. 2019. Fermentation. formulation and evaluation of PGPR Bacillus subtilis isolate as a bioagent for reducing the occurrence of peanut soil-borne diseases. J Integ Agric 18 (9) : 2080–2092.
Borriss R. 2011. Use of Plant-Associated Bacillus Strains as Biofertilizers and Biocontrol Agents in Agriculture. In: Maheshwari D.K. editor. Bacteria in Agrobiology: Plant Growth Responses. Springer; Berlin/Heidelberg. Germany: 41–76.
Chowdhury PS, Hartmann A, Gao XW, Borriss R. 2015. Biocontrol mechanism by root-associated Bacillus amyloliquefaciens FZ-B42. Front Microbiol 6 (780): 1- 11.
Costa L, Morandi M, Stricker S, Bettiol W. 2016. UV-B radiation reduces biocontrol ability of Clonostachys rosea against Botrytis cinerea. Biocont Sci Techn 26 (12):1736–1749.
Domingues M, Moura K, Salomão D, Elias L, Patricio F. 2016. Effect of temperature on mycelial growth of Trichoderma Sclerotinia minor and S. sclerotiorum. as well as on mycoparasitism. Summa Phytopathol 42 (3): 222 –227.
Douriet-Gámez NR. Maldonado-Mendoza IE. Ibarra-Laclette E. Blom J. Calderón-Vázquez CL. 2018.Genomic analysis of Bacillus sp. strain b25. a biocontrol agent of maize pathogen Fusarium verticillioides. Curr Microbiol 75: 247–255.
EPA [United State Environmental Protection Agency]. 2022. Ingredients Used in Pesticide Products: Pesticides. What Are Biopesticides? Available online: https://www.epa.gov/ ingredients-used-pesticide-products/what-are-biopesticides.
Hang NT, Oh S, Kim GH. Hur J. Koh Y J. 2005. Bacillus subtilis as a biocontrol agent against Botrytis cinerea in strawberries. Plant Pathol J. 21. 59–63.
Harca NN. 2015. Isolation and identification of nitrogen-fixing and producing indole acetic acid bacteria from oil palm plantation in Jambi. M.Sc. Thesis. Bogor Agricultural University. I.D.
Howell CR. 2001. Mechanisms employed by Trichoderma species in the biological control of plant diseases: the history and evolution of current concepts. Plant Dis 87: 4-10.
Kamilova F, Validov S, Azarova T, Mulders I, Lugtenberg B. 2005. Enrichment for enhanced competitive plant root tip colonizers selects for a new class of biocontrol bacteria. Environ Microbiol 7:1809–1817.
Kuan KB, Othman R, Abdul Rahim K, Shamsuddin ZH. 2016. Plant growth-promoting rhizobacteria inoculation to enhance vegetative growth. nitrogen fixation and nitrogen remobilization of maize under greenhouse conditions. PLoS ONE. 16;11:e0152478.
Kumar S, Manibhushan T, Archana R. 2014. Trichoderma: Mass production. formulation. quality control. delivery and its scope in commercialization in India for the management of plant diseases. Afr J Agric Res 9 (53): 3838-3852.
Li Y, Chi LD, Mao LG, Yan DD, Wu ZF, Ma TT, Guo MX, Wang QX, Ouyang CB, Cao AC. 2014. First report of ginger rhizome rot caused by Fusarium oxysporum in China. Plant Dis 98 (2): 282.
Locatelli G, Dos Santos G, Botelho P, Luna C, Avallone L. 2018. Development of Trichoderma sp. formulations in encapsulated granules (CG) and evaluation of conidia shelf-life. Biol Cont 117:21 –29.
Mia MB, Shamsuddin Z, Mahmood M. 2010. Use of plant growth-promoting bacteria in banana: a new insight for sustainable banana production. Int J Agric Biol 12 (3): 459-467
Miljakovic D, Jelena M, Svetlana BT. 2020. The Significance of Bacillus spp. in Disease Suppression and Growth Promotion of Field and Vegetable Crops. Micro 8 (7): 1037.
Mus F. Crook MB. Garcia K. Garcia CA. Geddes BA. Kouri ED. Paramasivan P. Ryu MH. Oldroyd GED. Poole PS. 2016. Symbiotic nitrogen fixation and the challenges to its extension to nonlegumes. Appl Environ Microbiol 2016;82:3698–3710.
Papavizas GC, Dunn MT, Lewis JA, Beagle-Ristaino J. 1984. Liquid fermentation technology for experimental production of biocontrol fungi. Phytopathology 74: 1171-1175.
Paul DP, Sinha SN. 2017. Isolation and characterization of phosphate solubilizing bacterium Pseudomonas aeruginosa KUPSB12 with antibacterial potential from river Ganga. India. Annals of Agra Sci 15 (1): 130-136.
Richardson AE, Simpson RJ. 2011. Soil microorganisms mediating phosphorus availability. Plant Physiol 156: 989–996.
Roger F, Keinath A. 2010. Bio fungicides and chemicals for managing diseases in organic vegetable production. Clemson University Cooperative Ext. Information Leaflet 88. Retrieved from http://www.clemson.edu/psapublishing/PAGES/PLNTPATH/IL88
Safari-Motlagh MR, Samimi Z. 2013. Evaluation of Trichoderma spp.. as biological agents in some of plant pathogens. Ann Biol Res 4: 173–179.
Seenivasagan R, Babalola OO. 2021. Utilization of Microbial Consortia as Biofertilizers and Biopesticides for the Production of Feasible Agricultural Product. Biol 10 : 1111-1116.
Sharma SB, Sayyed RZ, Trivedi MH, Gobi TA. 2013. Phosphate solubilizing microbes: Sustainable approach for managing phosphorus deficiency in agricultural soils. Springerplus. 2:587–600.
Shen J, Yuan L, Zhang J, Li H, Bai Z, Chen X, Zhang W, Zhang F. 2011. Phosphorus dynamics: From soil to plant. Plant Physiol. 156: 997–1005.
Slama HB, Hafsa CS, Ali CB, Mallique Q. 2019. Screening for Fusarium Antagonistic Bacteria From Contrasting Niches Designated the Endophyte Bacillus halotolerans as Plant Warden Against Fusarium. Front Microbiol 9 (3236).
Slepecky RA, Henphill HR. 2006. The Genus Bacillus-Nonmedical. In : Balows A. Trupper HG. Dworkin M. Harder W. Schleifer KH. editor. The Prokaryotes. Springer-Verlag. New York: 1663- 1696.
Supriyanto, Purwanto, SH Poromarto, Supyani. 2020. Evaluation of in vitro antagonistic activity of fungi from peatlands against Ganoderma species under acidic condition. Biodiversitas 21 (7) : 2935-2945.
Swaminathan J, van Koten C, Henderson H, Jackson T, Wilson M. 2016. Formulations for delivering Trichoderma atroviridae spores as seed coatings. effects of temperature and relative humidity on storage stability. J Appl Microbiol 120 (2):425 –431.
Ting ASY, Jioe E. 2016. In vitro assessment of antifungal activities of antagonistic fungi towards pathogenic Ganoderma boninense under metal stress. Biol Cont 96: 57-63.
Vey A, Hoagland RE, Butt TM. 2021. Toxic metabolites of fungal biocontrol agents. In: Butt TM. Jackson C. Magan N (eds) Fungi as biocontrol agents: Progress. problems and potential. CAB International. Bristol: 311-346.
Xie GH, Su BL, Cui ZJ. 1998. Isolation and identification of N2-fixing strains of Bacillus in rice rhizosphere of the Yangtze River valley. Acta Microbiol Sin. 38: 480–483.
Zou XH. Nonogaki H. Welbaum GE. 2002. A gel diffusion assay for visualization and quantification of chitinase activity. J Mol Biotech 22(1):19-27.