Morphological identification of the squirrel (Callosciurus notatus) tongue through Scanning Electron Microscopy (SEM) and histochemistry

##plugins.themes.bootstrap3.article.main##

EMILIA IKA MEGAWATI
STANISLAUS IVAN DAVIN PRADIPTA
ULFAH DAMIA
ULAYATUL KUSTIATI
HEVI WIHADMADYATAMI
DWI LILIEK KUSINDARTA

Abstract


Abstract. Megawati EI, Pradipta SID, Damia U, Kustiati U, Wihadmadyatami H, Kusindarta DL. 2023. Morphological identification of the squirrel (Callosciurus notatus) tongue through Scanning Electron Microscopy (SEM) and histochemistryBiodiversitas 242302-2314Squirrel (Callosciurus notatus) plays an important role in the agricultural ecosystem.Their urine and feces kill coconut tree pests and help preserve coconut farming productivity. This research focuses on the morphological identification of the tongue and lingual papillae distribution of the Callosciurus notatus’stongue. The tongue morphology and lingual papillae distribution of Callosciurus notatus were investigated through two different methods: using scanning electron microscopy (SEM) and histology. This paper provides macroscopic and histological tongue morphology and lingual papillae distribution of Callosciurus notatus. Macroscopically, the tongue is divided into the apex, corpus, and radix. The dorsal surface of the apex the groove and lateral prominence were occur. SEM and light microscopy observation showed that the apex bears three types of papillae: leaf-like filiform, bifid filiform, and fungiform. The corpus includes wide leaf-like filiform papillae, saw-like filiform papillae, bifid filiform papillae, and fungiform papillae. On the radix, there are conical like filiform papillae, fungiform papillae, conical and vallatae papillae. The lateral part of radix showed Weber’s glands that react strongly positively by Alcian Blue (AB) pH 2.5 and periodic acid Schiff (PAS) staining; Weber’s glands consist of neutral and acidic mucin. Von Ebner’s glands were at the textus muscularis striatus syncytialis near the vallatae papillae, which react weakly positively to PAS stain and did not react to AB pH 2.5 stain; von Ebner’s glands consist of neutral mucin. Masson Trichromestain revealed collagen structure on lamina propria mucosae and textus muscularis striatus syncytialis of apex; moderately on lamina propria mucosae and lesser on textus muscularis striatus syncytialis of the corpus and radix.


##plugins.themes.bootstrap3.article.details##

References
Abumandour MMA El-Bakary RMA. 2013. Morphological and scanning electron microscopic studies of the tongue of the Egyptian fruit bat (Rousettus aegyptiacus) and their lingual adaptation for its feeding habits. Veterinary Research Communications 37(3): 229–238. DOI:10.1007/s11259-013-9567-9
Abumandour MMA. 2014. Morphological Comparison of the Filiform Papillae of New Zealand White Rabbits (Oryctolagus cuniculus) as Domestic Mammals and Egyptian Fruit Bat (Rousettus aegyptiacus) as Wild Mammals Using Scanning Electron Microscopic Specimens. International Journal of Morphology 32(4): 1407–1417. DOI:10.4067/S0717-95022014000400045
Andalisa L, Rizaldi R, Nurdin J. 2018. Estimation of Kapapa Bajing Population (Callosciurus notatus Boddaert, 1785) Family Sciuridae in Nagari Koto Dalam, Padang Sago, Sumatera Barat. Metamorfosa: Journal of Biological Sciences 213(2): 210–213. DOI: 10.24843/metamorfosa.2018.v05.i02.p11
Bacha WJ, Bacha LM. 2000. Color Atlas of Veterinary Histology Second Edition. Lippincott Williams and Wilkins USA
Baker N. 2016. Plantain Squirrel – Callosciurus notatus. Ecology Asia 8235.
Ciena AP, Bolina CDS, Almeida SRYD, Rici REG, Oliveira MFD, Silva MCPD, Miglino MA, Watanabe I. 2013. Structural and ultrastructural features of the agouti tongue (Dasyprocta aguti Linnaeus 1766). Journal of Anatomy 223(2): 152–158. DOI:10.1111/joa.12065
Ciena AP, Santos ACD, Vasconcelos BG, Rici REG, Neto ACDA, Almeida SRYD, Miglino MA, Watanabe I. 2017. Morphological characteristics of the papillae and lingual epithelium of guinea pig (Cavia porcellus). Acta Zoologica 100(1): 53–60. DOI:10.1111/azo.12230
Damia U, Anjani A, Wihadmadyatami H, Kusindarta DL. 2021. Identification of the Lingual Papillae in the sugar glider ( Petaurus breviceps ) by scanning electron microscopy and light microscopy. Anatomia, Histologia, Embryologia. 50. 10.1111/ahe.12734.
Davydova L, Tkach G, Tymoshenko A, Moskalenko A, Sikora V, Kyptenko L, Lyndin M, Muravskyi D, Maksymova O, Suchonos O. 2017. Anatomical and morphological aspects of papillae epithelium muscles and glands of rats’ tongue: Light scanning and transmission electron microscopic study. Interventional Medicine and Applied Science 9(3): 168–177. DOI:10.1556/1646.9.2017.21
Emura S, Tamada A, Hayakawa D, Chen H, Jamali M, Taguchi H, Shoumura S. 1999. SEM study on the dorsal lingual surface of the flying squirrel Petaurista leucogenys. Annals of Anatomy 181(5): 495–498. DOI:10.2535/ofaj.79.113
Emura S. 2019 Morphology of the Lingual Papillae of the Japanese lesser flying squirrel and four-toed hedgehog. Okajimas Folia Anatomica Japonica 96(1): 23–26. DOI:10.2535/ofaj.96.23
Eroschenko VP. 2017. diFiore’s Atlas of Histology With Functional Correlations 13ed. Lippincott Williams and Wilkins,
Fehrenbach MJ, Popowics T. 2015. Student workbook for Illustrated dental embryology histology and anatomy. 4th edn. SaunderElseiver, China.
Go?dziewska-Har?ajczuk K, Nawrot J.K, Barszcz K Marycz K, Nawara T, Modli?ska K, Stryjek R. 2018. Biological aspects of the tongue morphology of wild-captive WWCPS rats: a histological histochemical and ultrastructural study. Anatomical Science International 93(4): 514–532. DOI:10.1007/s12565-018-0445-y
Gunawan G, Saragih G.R, Umardani Y, Karnati S, Wihadmadyatami H, Kusindarta D.L. 2019. Morphological study of the lingual papillae in the fruit bat (Rousettus amplexicaudatus) by scanning electron microscopy and light microscopy. Journal of Veterinary Medicine Series C: Anatomia Histologia Embryologia (June): 1–11. DOI:10.1111/ahe.12509
Iwasaki SI, Erdo?an S, Asami T. (2019). Evolutionary Specialization of the Tongue in Vertebrates: Structure and Function. In: Bels, V., Whishaw, I. (eds) Feeding in Vertebrates. Fascinating Life Sciences. Springer, Cham. DOI:10.1007/978-3-030-13739-7_10
Iwasaki SI, Miyata K, Kobayashi K. 1987. Comparative studies of the dorsal surface of the tongue in three mammalian species by scanning electron microscopy. Cells Tissues Organs 128(2): 140–146. DOI:10.1159/000146330
Iwasaki SI, Yoshizawa H, Aoyagi H. 2012. Localization of type III collagen in the lingual mucosa of rats during the morphogenesis of circumvallate papillae. Odontology 100:10-21. DOI: 0.1007/s10266-011-0020-7
Jackowiak H, Godynicki S. 2005. The distribution and structure of the lingual papillae on the tongue of the bank vole Clethrionomys glareolus. Folia Morphologica 64(4): 326–333.
Kobayashi S, Jackowiak H, Frackowiak H, Yoshimura K, Kumakura M, Kobayashi K. 2005. Scanning Electron Microscopic Study on the Lingual Papillae in the Manchurian Chipmunk Tamias sibiricus asiaticus. Okajimas Folia Anat. Jpn 69(2-3): 139-144
König HE, Liebich HG. 2020. Veterinary Anatomy of Domestic Animals. 7th edn. Scatthauer, Germany.
Nikumbh RD, Nikumbh DB, Umarji BN. 2012. Mucin Histochemical Study of The Colon in Normal and Malignant Lesions. International Journal of Health Sciences and research 2 (7): 20-32.
Pastor JF, Barbosa MFJ, Paz DM, Garcia M, Ferrero E. 2011. Functional and comparative study of lingual papillae in four species of bear (Ursidae) by scanning electron microscopy. Microscopy Research and Technique 74(10): 910–919. DOI:10.1002/jemt.20975
Reginato GDS, Bolina CDS, Watanabe I, Clena AP. 2014. Three-dimensional aspects of the lingual papillae and their connective tissue cores in the tongue of rats: A scanning electron microscope study. Scientific World Journal. DOI:10.1155/2014/841879
Sadeghinezhad J, Tootian, Javadi F. 2018. Anatomical and histological structure of the tongue and histochemical characteristics of the lingual salivary glands in the Persian squirrel (Sciurus anomalus). Anatomical Science International 93(1): 58–68. DOI:10.1007/s12565-016-0367-5
Sakr SMI, Taki-El-Deen FMA, Aboelwafa HR. 2013. Comparative light and scanning electron microscopic study of the lingual papillae in three different mammalian animals, Hemiechinus auritus (Erinaceomorpha: Erinaceidae) Cavia porcellus (Rodentia: Caviidae) and Mustela nivalis vulgaris (Carnivora: Mustel). Life Science Journal 10(4): 3082–3093.
Salim. 2013. Hama-hama yang Menyerang Bunga dan Buah Kelapa (Cocos nucifera L.) serta Pengendaliannya. Balai Penelitian Tanaman Palma: 211–221.
Saragih G, Gunawan G, Umardani Y, Karnati S, Kusindarta DL, WihadmadyatamiH. 2020. Morphological and scanning electron microscopic study of the lingual papillae in the Javan Pipistrelle (Pipistrellus javanicus). Anatomia, Histologia, Embryologia. 49. 10.1111/ahe.12566.
Singh KKP, Sandhya K. 2018. Comparative Histological Study on Lingual Glands of Some Mammals. Journal of Dental and Medical Sciences 17(8): 22-28.
Suvarna SK, Layton C, Bancroft JD. 2019. Bancroft’s Theory and Practice of Histological Techniques. Churcill Livingstone Elsevier, China.
Thorington R, Ferrel K. 2006. Squirrels: the animal answer guide. The Johns Hopkins University Press, USA.
Thorington RW, Koprowski J.J.L, Steele M.A, Whatton J.F. 2012. Squirrels of the world. The Johns Hopkins University Press, Bartimole.
Wolczuk K. 2014. Dorsal Surface of the Tongue of the Hazel Dormouse Muscardinus Avellanarius?. Scanning Electron and Light Microscopic Studies. 59(1–4): 35–47. DOI:10.2478/zoop-2014-0004