The characteristics of PUFAs-rich virgin fish oil as affected by size of tuna eye

##plugins.themes.bootstrap3.article.main##

WINI TRILAKSANI
https://orcid.org/0000-0002-4186-9656
BAMBANG RIYANTO
https://orcid.org/0000-0003-4131-1860
WAHYU RAMADHAN
https://orcid.org/0000-0003-1289-215X
FAHRI SINULINGGA
SILVA FAUZIAH

Abstract

Abstract. Trilaksani W, Riyanto B, Ramadhan W, Sinulingga F, Fauziah S. 2023. The characteristics of PUFAs-rich virgin fish oil as affected by size of tuna eye. Biodiversitas 24: 6545-6556. The food supplement market expects growth in demand for long-chain omega-3 fatty acids, particularly Eicosapentaenoic Acid (EPA) and Docosahexaenoic Acid (DHA), which play crucial for brain development and can lower the chances of health issues like depression, Myocardial Infarction (MI), thromboembolism, and cardiac arrhythmias, and have anti-viral effects, boosting the immune system during the pandemic. Therefore, to meet this demand, exploring new sources is crucial. Tuna eyes have emerged as a potential source of DHA; however, quality and standardized sizes pose a challenge as they are by-products. The purpose of this research was to determine how the eye size of tuna affected the quality and yield of fish oil, which is a novel source of omega-3 fatty acids. This study determined the virgin oil profile of tuna eyes, which met the quality standards. The investigation covered morphology, chemical composition, heavy metal content, oil yield and quality, fatty acid composition, and related health lipid indices (Atherogenicity Index (AI) and Thrombogenicity Index (TI)). For the size specifications, tuna eyes were classified into three groups based on their diameter, namely 'small' (<6 cm), 'medium' (6-9 cm), and 'large' (>9 cm). The results showed that eye size directly influenced the weight, fat content, and oil yield, with negligible disparity in chemical composition except for the fat content; the larger the eyes, the higher the oil yield. Tuna eye oil had a safe composition for consumption with low or undetected heavy metals, the oil's oxidative level met CODEX standards, and the fatty acid profile revealed DHA as the most abundant fatty acid, reaching 36.95%. The predominance of n-3 series values in the fatty acid composition of tuna's eye was 9-12 times greater than that of n-6 fatty acid values. The values of AI and TI were 0.33 and 0.12; 0.47 and 0.20; 0.55 and 0.20 for large, small, and medium eyes, respectively. The study highlights tuna eyes' unique characteristics and potential applications in the food supplement industry.

##plugins.themes.bootstrap3.article.details##

References
[AOAC] Association of Official Analytical Chemist. 2005. Official Methods of Analysis of the Association of Official Analytical of Chemist. The Association of Analytical Chemist, Inc., Virginia, US.
[CAC] Codex Alimentarius Commission. 1995. Standard for Contaminants and Toxins in Food and Feed CODEX STAN 193-1995. Food and Agriculture Organization of the United Nations, Rome.
[CAC] Codex Alimentarius Commission. 1999. Standard for Named Vegetable Oils CODEX-STAN 210 - 1999. Food and Agriculture Organization of the United Nations, Rome.
[CAC] Codex Alimentarius Commission. 2017. Standard for Fish Oils CODEX STAN 329-2017. Food and Agriculture Organization of the United Nations, Rome.
[EFSA] European Food Safety Authority. 2011. Scientific Opinion on the substantiation of health claims related to olive oil and maintenance of normal blood LDL-cholesterol concentrations (ID 1316, 1332), maintenance of normal (fasting) blood concentrations of triglycerides (ID 1316, 1332), maintenance of normal blood HDL-cholesterol concentrations (ID 1316, 1332) and maintenance of normal blood glucose concentrations (ID 4244) pursuant to Article 13(1) of Regulation (EC) No 1924/2006 EFSA Panel on Dietetic Products, Nutrition, and Allergies (NDA). EFSA Journal. Parma (IT): European Food Saf Authority 9 (4): 2044. DOI: 10.2903/j.efsa.2011.2044.
[FAO] Food and Agriculture Organization of the United Nations. 2013. Expert Consultation on the Risks and Benefits of Fish Consumption, GLOBEFISH, January, 2013. https://www.fao.org/3/bb211e/bb211e.pdf.
[GVR] Grand View Research. 2021. Dietary Supplements Market Size Worth $272.4 Billion By 2028. www.grandviewresearch.com/press-release/global-dietary-supplements-market.
[OEC] The Observatory of Economic Complexity. 2019. Fish Oil (HS: 1504) Product Trade, Exporters and Importers. https://oec.world/en/profile/hs92/fish-oil.
Aitta E, Marsol-Vall A, Damerau A, Yang B. 2021. Enzyme-assisted extraction of fish oil from whole fish and by-products of baltic herring (Clupea harengus membras). Foods 10 (8): 1811. DOI: 10.3390/foods10081811.
Alagawany M, Attia YA, Farag MR, Elnesr SS, Nagadi SA, Shafi ME, Khafaga AF, Ohran H, Alaqil AA, Abd El-Hack ME. 2021. The Strategy of boosting the immune system under the COVID-19 pandemic. Front Vet Sci 7: 570748. DOI: 10.3389/fvets.2020.570748.
Annabi A, Said K, Messaoudi I. 2013. Heavy metal levels in gonad and liver tissues effects on the reproductive parameters of natural populations of Aphanius facsiatus. Environ Sci Pollut Res Intl 20 (10): 7309-7319. DOI: 10.1007/s11356-013-1745-3.
Araújo CVM, Cedeño-Macias LA. 2016. Heavy metals in yellowfin tuna (Thunnus albacares) and common dolphinfish (Coryphaena hippurus) landed on the Ecuadorian coast. Sci Total Environ 541: 149-154. DOI: 10.1016/j.scitotenv.2015.09.090.
Asikin AN, Kusumaningrum I. 2018. Characteristics of snakehead fish protein extract based on the size of the weight of fish from the Mahakam watershed, East Kalimantan. Jurnal Pengolahan Hasil Perikanan Indonesia 21 (1): 137-142. DOI: 10.17844/jphpi.v21i1.21462. [Indonesian]
Baek N. 2012. Effects of Natural Antioxidants on Lipid Oxidatiom of Menhaden Oil. [Thesis]. Virginia polytechnic Institute and State University, Virginia, US.
Balakrishnan J, Kannan S, Govindasamy A. 2021. Structured form of DHA prevents neurodegenerative disorders: A better insight into the pathophysiology and the mechanism of DHA transport to the brain. Nutr Res 85: 119-134. DOI: 10.1016/j.nutres.2020.12.003.
Blanchet C, Lucas M, Julien P, Morin R, Gingras S, Dewailly E. 2005. Fatty acid composition of wild and farmed Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss). Lipids 40 (5): 529-531. DOI: 10.1007/s11745-005-1414-0.
Calder PC, Carr AC, Gombart AF, Eggersdorfer M. 2020. Optimal nutritional status for a well-functioning immune system is an important factor to protect against viral infections. Nutrients 12 (4): 1181. DOI: 10.3390/nu12041181.
Carr I, Glencross B, Santigosa E. 2023. The importance of essential fatty acids and their ratios in aquafeeds to enhance salmonid production, welfare, and human health. Front Anim Sci 4: 1147081. DOI: 10.3389/fanim.2023.1147081.
Chen J, Liu H. 2020. Nutritional indices for assessing fatty acids: A mini-review. Intl J Mol Sci 21 (16): 5695. DOI: 10.3390/ijms21165695.
Chew SC, Nyam KL. 2019. Refining of edible oils. In: Galanakis CM (eds). Lipids and Edible Oils: Properties, Processing and Applications. Academic Press, Cambridge. DOI: 10.1016/B978-0-12-817105-9.00006-9.
Clodoveo ML, Hbaieb RH. 2013. Beyond the traditional virgin olive oil extraction systems: Searching innovative and sustainable plant engineering solutions. Food Res Intl 54 (2): 1926-1933. DOI: 10.1016/j.foodres.2013.06.014.
Diao Y, Deng A, Wang J, Hong J. 2017. Vitamin A palmitate eye gel improves the density of conjunctival goblet cells and the production of mucin-5 subtype AC in rabbits with dry eye syndrome. Intl J Clin Exp Med 10 (12): 16181-16188.
DiNicolantonio JJ, O'Keefe JH. 2018. Importance of maintaining a low omega-6/omega-3 ratio for reducing inflammation. Open Heart 5 (2): e000946. DOI: 10.1136/openhrt-2018-000946.
Echeverría F, Valenzuela R, Hernandez-Rodas MC, Valenzuela A. 2017. Docosahexaenoic Acid (DHA), a fundamental fatty acid for the brain: New dietary sources. Prostaglandins Leukot Essent Fatty Acids 124: 1-10. DOI: 10.1016/j.plefa.2017.08.001.
Fadiyah NN, Megawati G, Luftimas DE. 2022. Potential of Omega 3 supplementation for Coronavirus Disease 2019 (COVID-19): A scoping review. Intl J Gen Med 15: 3915-3922. DOI: 10.2147/IJGM.S357460.
Gam LH, Leow CY, Baie S. 2005. Amino acid composition of snakehead fish (Channa striatus) of various sizes obtained at different times of the year. Malays J Pharmaceut Sci 3: 19-30.
Gamarro EG, Orawattanamateekul W, Sentina J, Gopal TKS. 2013. By-Product of Tuna Processing. FAO Globefish, Rome.
García-Moreno PJ, Guadix A, Go?ez-Robledo L, Melgosa M, Guadix EM. 2013. Optimization of bleaching conditions for sardine oil. J Food Eng 116 (2): 606-612. DOI: 10.1016/j.jfoodeng.2012.12.040.
Ghaeni M, Ghahfarokhi KN, Zaheri L. 2013. Fatty acids profile, atherogenic (IA) and thrombogenic (IT) health lipid indices in Leiognathusbindus and Upeneussulphureus. J Mar Sci Res Dev 3: 138. DOI: 10.4172/2155-9910.1000138.
Hathaway D, Pandav K, Patel M, Riva-Moscoso A, Singh BM, Patel A, Min ZC, Singh-Makkar S, Sana MK, Sanchez-Dopazo R, Desir R, Fahem MMM, Manella S, Rodriguez I, Alvarez A, Abreu R. 2020. Omega 3 fatty acids and COVID-19: A comprehensive review. Infect Chemother 52 (4): 478-495. DOI: 10.3947/ic.2020.52.4.478.
International Fish Oil Standard. 2014. Fish Oil Purity Standards. www.Omegavia.com/best.
Jeffrey BG, Weisinger HS, Neuringer M, Mitchell DC. 2001. The role of docosahexaenoic acid in retinal function. Lipids 36 (9): 859-871. DOI: 10.1007/s11745-001-0796-3.
Jeong D-H, Kim K-B-W-R, Kim M-J, Kang B-K, Ahn D-H. 2016. Skipjack tuna (Katsuwonus pelamis) eyeball oil exerts an anti-inflammatory effect by inhibiting NF-?B and MAPK activation in LPS-induced RAW 264.7 cells and croton oil-treated mice. Intl Immunopharmacol 40: 50-56. DOI: 10.1016/j.intimp.2016.07.005.
Kaushik P, Dowling K, Barrow CJ, Adhikari B. 2015. Microenchapculation of omega-3 fatty acids: a review of microencaptulation and characterization methods. J Funct Foods 19: 868-881. DOI: 10.1016/j.jff.2014.06.029.
Khalid W, Gill P, Sajid Arshad M, Ali A, Ranjha MMAN, Mukhtar S, Afzal F, Maqbool Z. 2022. Functional behavior of DHA and EPA in the formation of babies brain at different stages of age, and protect from different brain-related diseases. Intl J Food Prop 25 (1): 1021-1044. DOI: 10.1080/10942912.2022.2070642.
Khoshnoudi-Nia S, Forghani Z, Jafari SM. 2022. A systematic review and meta-analysis of fish oil encapsulation within different micro/nanocarriers. Crit Rev Food Sci Nutr 62 (8): 2061-2082. DOI: 10.1080/10408398.2020.1848793.
Ku JE, Lee SI, Kim DN. 2021. Age and growth of southern bluefn tuna, Thunnus maccoyii, based on otolith microstructure. Ocean Sci J 56: 413-423. DOI:10.1007/s12601-021-00041-z.
Kuratko CN, Barrett EC, Nelson EB, Salem NJr. 2013. The relationship of Docosahexaenoic Acid (DHA) with learning and behavior in healthy children: A review. Nutrients 5: 2777-2810. DOI: 10.3390/nu5072777.
La Dia WNA, Trilaksani W, Ramadhan W. 2022. Purification of fish oil tuna eyes (Thunnus sp.) rich-DHA with various adsorbents. Jurnal Pengolahan Hasil Perikanan Indonesia 25 (3): 428-440. DOI: 10.17844/jphpi.v25i3.42794. [Indonesian]
Lad EM, Cousins SW, Van Arnam JS, Proia AD. 2015. Abundance of infiltrating CD163+ cells in the retina of postmortem eyes with dry and neovascular age-related macular degeneration. Graefes Arch Clin Exp Ophthalmol 253 (11): 1941-1945. DOI: 10.1007/s00417-015-3094-z.
Lu D, Lin Q, Zhu J, Zhang F. 2023. Effects of aging uncertainty on the estimation of growth functions of major tuna species. Fishes 8 (3): 131. DOI: 10.3390/fishes8030131.
Luthfiah, Genisa J, Metusalach M, Abustam E, Sabahannur S, Ramadhan W. 2014. Analysis quality fish oil (Decapterus sp.) containing omega-3 using extraction method (Steam Press) and (Bligh and Dyer). Adv Environ Biol 8 (22): 923-930.
Mahesar SA, Sherazi STH, Khaskheli AR, Kandhro AA, Sirajuddin. 2014. Analytical approaches for free fatty acids assessment in oils and fats. Anal Methods 6 (14): 4956-4963. DOI: 10.1039/c4ay00344f.
Mason RP, Libby P, Bhatt DL. 2020. Arteriosclerosis, thrombosis, and vascular biology emerging mechanisms of cardiovascular protection for the omega-3 fatty acid eicosapentaenoic acid. Arterioscler Thromb Vasc Biol 40 (5): 1135-1147. DOI: 10.1161/atvbaha.119.313286.
Maurya PK, Malik DS, Yadav KK, Kumar A, Kumar S, Kamyab H. 2019. Bioaccumulation and potential sources of heavy metal contamination in fish species in River Ganga basin: Possible human health risks evaluation. Toxicol Rep 6: 472-481. DOI: 10.1016/j.toxrep.2019.05.012.
Mkadem H, Kaanane A. 2019. Recovery and characterization of fish oil from by-products of Sardine (Sardina pilchardus) in the canning process. J Aquat Food Prod Technol 28 (10): 1037-1050. DOI: 10.1080/10498850.2019.1682733.
Mohanty BP, Ganguly S, Mahanty A, Sankar TV, Anandan R, Chakraborty K, Paul BN, Sarma D, Dayal JS, Venkateshwarlu G, Mathew S, Asha KK, Karunakaran D, Mitra T, Chanda S, Shahi N, Das P, Akhtar MS, Vijayagopal P, Sridhar N. 2016. DHA and EPA content and fatty acid profile of 39 food fishes from India. Biomed Res Intl 2016: 4027437. DOI: 10.1155/2016/4027437.
Motti ML, Tafuri D, Donini L, Masucci MT, De Falco V, Mazzeo F. 2022. The role of nutrients in prevention, treatment and post-Coronavirus Disease-2019 (COVID-19). Nutrients 14 (5): 1000. DOI: 10.3390/nu14051000.
Murado MA, Montemayor MI, Cabo ML, Vazquez JA, Gonzalez MP. 2012. Optimization of extraction and purification process of hyaluronic acid from fish eyeball. Food Bioproducts Process 90 (3): 491-498. DOI: 10.1016/j.fbp.2011.11.002.
Mustapha RA, Bolajoko O, Akinola OO. 2014. Omega-3 and Omega-6 fatty acids potential of smoked and boiled Catfish (Clarias gariepinus). Curr Res Nutr Food Sci 2 (2): 94-97. DOI: 10.12944/crnfsj.2.2.06.
Nag TC, Wadhwa S. 2012. Accumulation of lipid inclusions in astrocytes of aging human optic nerve. Acta Biol Hung 63 (Suppl. 1): 54-64. DOI: 10.1556/ABiol.63.2012.Suppl.1.6.
Nazir N, Diana A, Sayuti K. 2017. Physicochemical and fatty acid profile of fish oil from head of tuna (Thunnus albacares) extracted from various extraction method. Intl J Adv Sci Eng Inform Technol 7 (2): 709-715. DOI: 10.18517/ijaseit.7.2.2339.
Pertiwi NPD, Nugraha B, Sulistyaningsih RK, Jatmiko I, Sembiring A, Mahardini A, Cahyani NKD, Anggoro AW, Madduppa HH, Sutikno A, Barber PH, Mahardika GN. 2017. Short Communication: Lack of differentiation within the bigeye tuna population of Indonesia. Biodiversitas 18 (4): 1406-1413. DOI: 10.13057/biodiv/d180416.
Pratama WW, Nursyam H, Hariati AM, Islamy RA, Hasan V. 2020. Proximate analysis, amino acid profile and albumin concentration of various weights of Giant Snakehead (Channa micropeltes) from Kapuas Hulu, West Kalimantan, Indonesia. Biodiversitas 21 (3): 1196-1200. DOI: 10.13057/biodiv/d210346.
Renuka V, Anandan R, Suseela M, Ravishankar CN, Sivaraman GK. 2016. Fatty acid profile of yellowfin tuna eye (Thunnus albacares) and oil Sardine muscle (Sardinella longiceps). Fish Technol 53: 151-154.
Restrepo VR, Diaz GA, Walter JF, Neilson JD, Campana SE, Secor D, Wingate RL. 2010. Updated estimate of the growth curve of Western Atlantic bluefin tuna. Aquat Living Resour 23 (4): 335-342. DOI: 10.1051/alr/2011004.
Ruilova MB, Niño-Ruiz ZM, Arias-Toro D, Ruilova PS. 2022. Effect of drying and maturity on the antioxidant properties of the Blueberry (Vaccinium floribundum Kurth) from the Ecuadorian Moorland and sensory evaluation of its infusion. Curr Res Nutr Food Sci 10 (2): 575-583. DOI: 10.12944/CRNFSJ.10.2.14.
Sardenne F, Bodin N, Chassot E, Amiel A, Fouché E, Degroote M, Hollanda S, Pethybridge H, Lebreton B, Guillou G, Ménard F. 2016. Trophic niches of sympatric tropical tuna in the Western Indian Ocean inferred by stable isotopes and neutral fatty acids. Prog Oceanogr 146: 75-88. DOI: 10.1016/j.pocean.2016.06.001.
Shakoor H, Feehan J, Al Dhaheri AS, Ali HI, Platat C, Ismail LC, Apostolopoulos V, Stojanovska L. 2021. Immune-boosting role of vitamins D, C, E, zinc, selenium and omega-3 fatty acids: Could they help against COVID-19? Maturitas 143: 1-9. DOI: 10.1016/j.maturitas.2020.08.003.
Sidhu KS. 2003. Health benefits and potential risks related to consumption of fish or fish oil. Regul Toxicol Pharmacol 38 (3): 336-344. DOI: 10.1016/j.yrtph.2003.07.002.
Sittiprapaporn P, Bumrungpert A, Suyajai P, Stough C. 2022. Effectiveness of fish oil-DHA supplementation for cognitive function in Thai children: A randomized, double-blind, two-dose, placebo-controlled clinical trial. Foods 11 (17): 2595. DOI: 10.3390/foods11172595.
Soltan SSAM, Gibson RA. 2008. Levels of omega 3 fatty acids in Australian seafood. Asia Pac J Clin Nutr 17 (3): 385-390.
Stando M, Piatek P, Namiecinska M, Lewkowicz P, Lewkowicz N. 2020. Omega-3 polyunsaturated fatty acids EPA and DHA as an adjunct to non-surgical treatment of periodontitis: A randomized clinical trial. Nutrients 12 (9): 2614. DOI: 10.3390/nu12092614.
Suseno SH, Saraswati S, Hayati S, Izaki AF. 2014. Fatty acid composition of some potential fish oil from production centers in Indonesia. Orient J Chem 30 (3): 975-980. DOI:10.13005/ojc/300308.
Trilaksani W, Riyanto B, Azzahra F, Santoso J, Tarman K. 2020b. Recovery of tuna virgin fish oil and formulation as a product model of emulsion food supplement. IOP Conf Ser: Earth Environ Sci 414: 012027. DOI: 10.1088/1755-1315/414/1/012027.
Trilaksani W, Riyanto B, Syifa AL. 2020a. Extraction and microencapsulation of tuna virgin fish oil with mangrove fruit extract fortified into extrusion cereals. IOP Conf Ser: Earth Environ Sci 414: 012032. DOI: 10.1088/1755-1315/420/1/012032.
Ulbricht TL, Southgate DA. 1991. Coronary heart disease: Seven dietary factors. Lancet 338: 985-992. DOI: 10.1016/0140-6736(91)91846-m.
Van-Leeuwen EM, Emri E, Merle BMJ, Colijn JM, Kersten E, Cougnard-Gregoire A, Dammeier S, Meester-Smoor M, Pool FM, de-Jong EK, Delcourt C, Rodrigez- Bocanegra E, Biarnés M, Luthert PJ, Ueffing M, Klaver CCW, Nogoceke E, den-Hollander AI, Lengyel I. 2018. A new perspective on lipid research in age- related macular degeneration. Prog Retinal Eye Res 67 (1): 56-86. DOI: 10.1016/j.preteyeres.2018.04.006.
Verheyen D, Xu XM, Govaert M, Baka M, Skåra T, Van Impe JF. 2019. Food microstructure and fat content affect growth morphology, growth kinetics, and preferred phase for cell growth of listeria monocytogenes in fish-based model systems. Appl Environ Microbiol 85 (16): e00707-19. DOI: 10.1128/aem.00707-19.
Wei W, Hu M, Huang J, Yu S, Li X, Li Y, Mao L. 2021. Anti-obesity effects of DHA and EPA in high fat-induced insulin resistant mice. Food Funct 12 (4): 1614-1625. DOI: 10.1039/D0FO02448A.
Yamagata K. 2017. Docosahexaenoic acid regulates vascular endothelial cell function and prevents cardiovascular disease. Lipids Health Dis 16 (1): 118. DOI: 10.1186/s12944-017-0514-6.
Zhang X, Yuan T, Chen X, Liu X, Hu J, Liu Z. 2023. Effects of DHA on cognitive dysfunction in aging and Alzheimer's disease: The mediating roles of ApoE. Prog Lipid Res 93: 101256. DOI: 10.1016/j.plipres.2023.101256.
Zhang Y, Sun Q, Liu S, Wei S, Xia Q, Ji H, Deng C, Hao J. 2021. Extraction of fish oil from fish heads using ultra-high pressure pre-treatment prior to enzymatic hydrolysis. Innov Food Sci Emerg Technol 70: 102670. DOI: 10.1016/j.ifset.2021.102670.