Study of Curcuma diversity from Central Java, Indonesia for sunscreen and antioxidant activity based on quantitative phytochemical analysis

##plugins.themes.bootstrap3.article.main##

HENNY NURHASNAWATI
REKSI SUNDU
ANITA SUKMAWATI

Abstract

Abstract. Nurhasnawati H, Sundu R, Sukmawati A. 2023. Study of Curcuma diversity from Central Java, Indonesia for sunscreen and antioxidant activity based on quantitative phytochemical analysis. Biodiversitas 24: 6880-6887. Indonesia is home to various plants, including those of the genus Curcuma. This study aimed to determine the effect of total phenolic and flavonoid content of Curcuma on antioxidant activity and SPF values. It is known that this rhizome contains secondary metabolites such as flavonoids and phenols, which are known to have antioxidant potential and are believed to act as natural ingredients for sun protection products. The samples used in this study were rhizomes of five Curcuma species from Central Java, namely C. aeruginosa, C. longa, C. mango, C. xanthorrhiza, and C. zedoaria. Alkaloids, flavonoids and tannins are present in all samples. UV-Vis spectrophotometry was used with the following results: the flavonoid total content ranged from 0.94-243.5 mg QE/g, while the total phenolic from 11.27-109.23 mg GAE/g. The DPPH and ABTS test for antioxidant activity showed that C. longa had the highest activity with an IC50 of 78.79 and 0.4273 ppm. The highest SPF values in C. longa were 31.55-36.97 (high protection). The results of the data analysis using the Spearman correlation test revealed a significant correlation between the antioxidant activity and SPF with the flavonoid and phenolic content of the Curcuma extracts.

##plugins.themes.bootstrap3.article.details##

References
Akoglu H. 2018. User’s guide to correlation coefficients. Turkish Journal of Emergency Medicine 18: 91–93. DOI: 10.1016/j.tjem.2018.08.001.
Alafiatayo AA, Ahmad S, & Maziah M. 2014. Total antioxidant capacity, flavonoid, phenolic acid and polyphenol content in ten selected species of Zingiberaceae rhizomes. Afr J Tradit Complement Altern Med 11 (3): 7–13. DOI: 10.4314/ajtcam.v11i3.2.
Alok S, Jain SK, Verma A, Kumar M, Mahor A, & Sabharwal M. 2014. Herbal antioxidant in clinical practice: A review. Asian Pac J Trop Biomed 4 (1): 78–84. DOI: 10.1016/S2221-1691(14)60213-6.
Asyhar R, Minarni M, Arista RA, & Nurcholis W. 2023. Total phenolic and flavonoid contents and their antioxidant capacity of Curcuma xanthorrhiza accessions from Jambi. Biodiversitas 24 (9), 5007–5014. DOI: 10.13057/biodiv/d240944.
Blainski A, Lopes GC, & de Mello JCP. 2013. Application and analysis of the Folin Ciocalteu method for the determination of the total phenolic content from Limonium brasiliense L. Molecules 18: 6852–6864. DOI: 10.3390/molecules18066852.
Budiati A, Rahmat D, & Alwiyah Z. 2021. Antioxidant and sunscreen activity from nanoparticles extract of Temulawak rhizome (Curcuma xanthorrhiza Roxb.) and formulation in the form of a cream. Jurnal Jamu Indonesia 6 (2): 75–82. DOI: 10.29244/jji.v6i2.212.
Ebrahimzadeh MA, Enayatifard R, Khalili M, Ghaffarloo M, Saeedi M, & Charati JY. 2014. Correlation between Sun Protection Factor and antioxidant activity, phenol and flavonoid contents of some medicinal plants. Iranian Journal of Pharmaceutical Research 13 (3): 1041–1047.
Guan LL, Lim HW, & Mohammad TF. 2021. Sunscreens and photoaging: A review of current literature. American Journal of Clinical Dermatology 22 (6): 819–828. DOI: 10.1007/s40257-021-00632-5.
Kandhasamy R, Arts L, Ramnath S, & Venkataramegowda S. 2021. Phytochemical screening and antioxidant activity of leaves and stem bark extracts of Garcinia imberti - an endangered plant. International Journal of Pharmaceutical Sciences and Research 6 (9): 1000–1005. DOI: 10.2139/ssrn.3861545.
Khumaida N, Syukur M, Bintang M, & Nurcholis W. 2019. Phenolic and flavonoid content in ethanol extract and agro-morphological diversity of Curcuma aeruginosa accessions growing in West Java, Indonesia. Biodiversitas 20 (3): 656–663. DOI: 10.13057/biodiv/d200306.
Lefahal M, Zaabat N, Ayad R, Makhloufi EH, Djarri L, Benahmed M, Laouer H, Nieto G & Akkal S. 2018. In vitro assessment of total phenolic and flavonoid contents, antioxidant and photoprotective activities of crude methanolic extract of aerial parts of Capnophyllum peregrinum L. (Apiaceae) growing in Algeria. Medicines, 5 (26): 1–10. DOI: 10.3390/medicines5020026
Li J, Mao B, Tang X, Zhang Q, Zhao J, Zhang H, & Cui S. 2023. Protective effects of Naringenin and Apigenin in ameliorating skin. Foods 12: 1–15. DOI: 10.3390/foods12112120.
Maglio DHG, Paz ML, & Leoni J. 2016. Sunlight effects on immune system?: Is there something else in addition to UV-induced immunosuppression? BioMed Research International 2016: 1–10. DOI: 10.1155/2016/1934518.
Manuhara YSW, Sugiharto S, Kristanti AN, Aminah NS, Wibowo, AT, Wardana AP, Putro YK, & Sugiarso D. 2022. Antioxidant activities, total phenol, flavonoid, and mineral content in the rhizome of various Indonesian herbal plants. Rasayan Journal of Chemistry 15 (4): 2724–2730. DOI: 10.31788/RJC.2022.1548024.
Merin KA, Shaji M, & Kameswaran R. 2022. A review on sun exposure and skin diseases. Indian Journal of Dermatology 67 (5): 625. DOI: 10.4103/ijd.ijd_1092_20.
Musdalipah, Tee SA, Karmilah, Sahidin, Fristiohady A, Yodha AWM. 2021. Total phenolic and flavonoid content, antioxidant, and toxicity test with BSLT of Meistera chinensis fruit fraction from Southeast Sulawesi. Borneo Journal of Pharmacy 4 (1): 6–15. DOI: 10.33084/bjop.v4i1.1686
Nunes AR, Vieira IGP, Queiroz DB, Leal ALAB, Morais SM, Muniz DF, Calixto-Junior JT, & Coutinho HDM. 2018. Use of flavonoids and cinnamates, the main photoprotectors with natural origin. Advances in Pharmacological Sciences 2018: 1–9. DOI: 10.1155/2018/5341487.
Nurcholis W, Khumaida N, Syukur M, & Bintang M. 2016. Variability of total phenolic and flavonoid content and antioxidant activity among 20 Curcuma aeruginosa Roxb. accessions of Indonesia. Asian Journal of Biochemistry 11 (3): 142–148. DOI: 10.3923/ajb.2016.142.148.
Nurhasnawati H, Sundu R, Sapri, Supriningrum R, Kuspradini H, & Arung ET. 2019. Antioxidant activity, total phenolic and flavonoid content of several indigenous species of ferns in East Kalimantan, Indonesia. Biodiversitas 2 (2): 576–580. DOI: 10.13057/BIODIV/D200238.
Nurjannah L, Azhari A, & Supratman U. 2023. Secondary metabolites of endophytes associated with the Zingiberaceae family and their pharmacological activities. Scientia Pharmaceutica 91 (1): 1–24. DOI: 10.3390/scipharm91010003.
Panche, AN, Diwan AD, & Chandra SR. 2016. Flavonoids: an overview. Jornal of Nutritional Science 5 (e47): 1–15. DOI: 10.1017/jns.2016.41
P?kal A & Pyrzynska K. 2014. Evaluation of aluminium complexation reaction for flavonoid content assay. Food Anal. Methods 7: 1776–1782. DOI: 10.1007/s12161-014-9814-x.
Rahaman M, Rakib A, Mitra S, Tareq AM, Emran TB, Daula AFMSU, Amin MN, & Gandara JS. 2021. The genus Curcuma and inflammation: Overview of the pharmacological perspectives. Plants 10 (63): 1–19. DOI: 10.3390/plants10010063.
Sharma M, & Sharma A. 2023. A Review on nature based sunscreen agents. IOP Conference Series: Earth and Environmental Science 1110 (1): 1–11. DOI: 10.1088/1755-1315/1110/1/012047.
Subositi D, & Wahyono S. 2019. Study of the genus Curcuma in Indonesia used as traditional herbal medicines. Biodiversitas 20 (5): 1356–1361. DOI: 10.13057/biodiv/d200527.
Suryani, Al Anshory AC, Marlin, Artika IM, Ambarsari L, & Nurcholis W. 2022. Variability total phenolic content and antioxidant activity of Curcuma zanthorrhiza and C. aeruginosa cultivated in three different locations in West Java, Indonesia. Biodiversitas 23 (4): 1998–2003. DOI: 10.13057/biodiv/d230434.
Tungmunnithum D, Thongboonyou A, & Pholboon A. 2018. Flavonoids and other phenolic compounds from medicinal plants for pharmaceutical and medical aspects: an overview. Medicines 5 (93): 1–16. DOI: 10.3390/medicines5030093.
Widodo H, Sismindari S, Asmara W & Rohman A. 2019. Antioxidant activity, total phenolic and flavonoid contents of selected medicinal plants used for liver diseases and its classification with chemometrics. Journal of Applied Pharmaceutical Science 9 (06): 99–105. DOI: 10.7324/JAPS.2019.90614.

Most read articles by the same author(s)