Short Communication: Diversity of Chlorpyrifos-degrading bacteria isolated from shallow aquifer of East Java Coastal Settlements, Indonesia
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Abstract. Rochaddi B, Zainuri M, Sabdono A. 2019. Short Communication: Diversity of Chlorpyrifos-degrading bacteria isolated from shallow aquifer of East Java Coastal Settlements, Indonesia. Biodiversitas 20: 3662-3666. Wastes from inhabited regions, factories and maricultural practices through movement and penetration enter to groundwaters. A study was undertaken to determine the diversity of Chlorpyrifos-degrading bacteria isolated from a shallow aquifer of East Java coastal settlements. Fifteen of 116 bacterial strains isolated from shallow aquifer samples of 12 household wells were selected due to their capability of degrading Chlorpyrifos herbicide. These isolates were different in the ability of Chlorpyrifos degradation. They utilize Chlorpyrifos as the source of carbon and energy for their growth. Their initial degradation at 50?mg?L?1 concentration within the first 4 days ranged between 26.81 and 70.12 %. The 16S rRNA gene sequence analyses indicated that the majority of the isolates belonged to members of Bacillus genera. These bacterial strains were Bacillus cereus (seven strains) and Bacillus paramycoides (four strains). Besides, three strains were identified as Bacillus subtilis and one strain as Bacillus thuringiensis. Bacillus cereus strain LCA1.1 was selected for further study on kinetic growth and Chlorpyrifos utilization. These bacterial strains have a great potential utility for the bioremediation of shallow aquifer of coastal settlement contaminated with Chlorpyrifos pesticides.
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References
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Yang C, Liu N, Guo X, Qiao C. 2006. Cloning of mpd gene from a chlorpyrifos-degrading bacterium and use of this strain in
bioremediation of contaminated soil. FEMS Microbiol Lett 265:118–125
Zilda DS, Yulianti Y, Sholihah RF, Subaryono S, Fawzya YN, Irianto HE.2019. A novel Bacillus sp. isolated from rotten seaweed: identification and
characterization alginate lyase its produced. Biodiversitas 20: 1166-1172.
Microbiolology 47: 563–570.
Amin M, Rakhisi Z, Ahmady AZ. 2015. Isolation and identification of Bacillus species from soil and evaluation of their antibacterial
properties. Avicenna Journal of Clinical Microbiology and Infection, 2(1):10–13
Bendis RJ, Relyea RA, 2016. Wetland defense: naturally occurring pesticide resistance in zooplankton populations protects the stability of aquatic
communities, 181(2): 487-498
Devi SL, Khaund P, Joshi SR. 2010. Thermostable ?-amylase from natural variants of Bacillus spp. prevalent in eastern Himalayan range, African
Journal of Microbiological Research. 4: 2534–2542.
Eyles N, Meriano M, Chow-Fraser P. 2013. Impacts of European settlement (1840–present) in a Great Lake watershed and lagoon: Frenchman’s
Bay, Lake Ontario, Canada. Environ Earth Sci (2013) 68(8): 2211-2228 https://doi.org/10.1007/s12665-012-1904-8
Deng SY, Chen Y, Wang DS, Shi TZ, Wu XW, Ma X, Li XQ, Hua RM, Tang XY, Li QX.2015. Rapid biodegradation of organophosphorus
pesticides by Stenotrophomonas sp G1. Journal of Hazardous Materials 297:17–24
Horne I, Sutherland TD, Harcourt RL, Russell, RJ, Oakeshott JG. 2002. Identification of an OPD (Organophosphate Degradation) gene in an
Agrobacterium isolate. Applied Environmental Microbiology 68(1): 3371-3376
Isworo S, Purwanto I, Sabdono A. 2015. Impact of Pesticide Use on Organophosphorus and Organochlorine Concentration in Water
and Sediment of Rawa Pening Lake, Indonesia, Research Journal of Environmental Sciences, 9(5): 233-240.
Lakshmi C, Kumar M, Khanna S., 2009. Biodegradation of chlorpyrifos in soil enriched cultures. Current Microbiology 58 35-38.
Lapworth DJ, Das P, Shaw A, Mukherjee A, Civil W, Petersen JO, Gooddy DC, Wakefield O, Finlayson A, Krishan G, Sengupta P, MacDonald
AM. 2018. Deep urban groundwater vulnerability in India revealed through the use of emerging organic contaminants and residence time
Tracer. Environmental Pollution 240: 938-949
Li X, Jiang J, Gu L, Ali SW, He J, Li S. 2008. Diversity of chlorpyrifos-degrading bacteria isolated from chlorpyrifos-contaminated samples
International Biodeterioration & Biodegradation 62:331–335
Liu Z, Chen X, Shi Y, Su Z. 2012. Bacterial degradation of chlorpyrifos by Bacillus cereus. Advanced Materials Research 356(360):676–
680
Magda AE, Hamed HAMM. 2016 Potential of Bacillus isolates as bio-control agents against some fungal phytopathogens. Biocatalysis and Agricultural
Biotechnology 5:173–178.
Puspita ID, Wardani A, Puspitasari ROA, Nugraheni PS, Putra MMP, Pudjiraharti S, Ustadi. 2017. Occurrence of chitinolytic bacteria in shrimp rusip
and measurement of their chitin-degrading enzyme activities. Biodiversitas 18: 1275-1281.
Putnam RA, Nelson JO, Clark JM. 2003. The persistence and degradation of chlorothalonil and chlorpyrifos in a cranberry bog
Journal of agricultural and food chemistry 51(1):170-176
Racke, K. D., D. A. Laskowski, and M. R. Schultz. 1990. Resistance of paraquat dichlor to enhanced biodegradation in soil. J. Agric. Food Chem.
38:1430-1436.
Rayu S, Nielsen UN, Nazaries L, Singh BK. 2017. Isolation and Molecular Characterization of Novel Chlorpyrifos and 3,5,6trichloro-2-pyridinol-
degrading Bacteria from Sugarcane Farm Soils. Frontier in Microbiology 8:518. doi: 10.3389/fmicb.2017.00518
Relyea, RA. 2009. A cocktail of contaminants: how mixtures of pesticides at low concentrations affect aquatic communities. Oecologia, 159 (2):363-376
Rokade KB, Mali GV. 2013. Biodegradation of Chlorpyrifos by Pseudomonas desmolyticum NCIM 2112 International Journal of Pharma
and Bio Sciences 4(2): (B) 609 - 616
Sabdono, A., S. Kang, H.G. Hur, H.P. Grossart, M. Simon and O.K. Radjasa. 2007. Organophosphate Pesticide Concentration in Coral Tissues of Indonesian Coastal Waters. Pak. J. Biol. Sci. 10 (11): 1926-1929
Swofford, D. L. 1998. PAUP*. Phylogenetic Analysis Using Parsimony (*and Other Methods). Version 4. Sinauer Associates, Sunderland,
Massachusetts. Copyright © Smithsonian Institution, 1998
Singh, B. K., Walker, A., Morgan, J. A. W., Wright, D. J. (2004). Biodegradation of Paraquat dichlor by Enterobacter Strain B-14 and Its Use in
Bioremediation of Contaminated Soils. Appl. Environ. Microbiol. 70: 4855-4863
Shannon MA, Bohn PW, Elimelech M, Georgiadis JG, Marinas, BJ, Mayes AM. 2008. Science and technology for water purification in the coming
decades 452(20):301-310
Thompson, J.D., T.J. Gibson, F. Plewniak, F. Jeanmougi, and D.G. Higgins. 1997. The Clustal X-Window interface: Flexible strategies for multiple
alignment through sequence alignment aided by quality analysis tools. Nucl. Acids Res. 25: 4876-4882.
Wang S, Zhang C, Li K, Qu J, Shi Y, Yan Y. 2013. Chlorpyrifos-induced stress response in the chlorpyrifos-degrader Klebsiella sp. CPK.
International Biodeterioration & Biodegradation 82:17-23
Yang L, Zhao Y, Zhang B, Yang C, Zhang X. 2005. Isolation and characterization of a chlorpyrifos and 3,5,6-trichloro-2-pyridinol degrading
bacterium, FEMS Microbiology Letters, 251 (1):67–73, https://doi.org/10.1016/j.femsle.2005.07.031
Yang C, Liu N, Guo X, Qiao C. 2006. Cloning of mpd gene from a chlorpyrifos-degrading bacterium and use of this strain in
bioremediation of contaminated soil. FEMS Microbiol Lett 265:118–125
Zilda DS, Yulianti Y, Sholihah RF, Subaryono S, Fawzya YN, Irianto HE.2019. A novel Bacillus sp. isolated from rotten seaweed: identification and
characterization alginate lyase its produced. Biodiversitas 20: 1166-1172.
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